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71.
福建深沪湾潮间带全新世有孔虫及其环境意义 总被引:2,自引:1,他引:2
本文对深沪湾西部潮间带表层沉积物和两个钻孔岩芯共30个样品的有孔虫组成进行了定性和定量分析。结果表明,表层沉积物中有孔虫群分布规律明显,随着离岸向海越远,水深越大,有孔虫娄量从1倍数上升到5位数,种数为9-69种,多变度V31-44,复合分异度H(s)3.25-3.25。从钻孔岩芯中的全新世有孔虫群特征可看出,中全新世以来海面升降和海洋环境可分为2个阶段:(1)海水初到钻孔位置,有孔虫数量和种数较少,分异度不高,海相性程度较低。(2)后期(埋深1.8m和2.0m以浅),海面有所升高,变为潮间带(高潮位→中潮位→低潮位)环境。 相似文献
72.
一种用磷酸钙法高效转染HEK 293T细胞方法的建立 总被引:1,自引:0,他引:1
利用磷酸钙转染法将lacZ-pShuttle质粒导入HEK 293T细胞中,实验证明,pH值、DNA纯度、转染后培养时间和甘油休克时间是影响转染效率的重要因素.转染后培养6h进行甘油休克效果最佳,比培养0h直接进行休克转染效率升高584.4%;甘油休克时间为4.5min时转染效果最佳,比不用甘油休克转染效率升高130.8%.通过对影响转染的几个条件进行优化,建立了1种可以高效转染HEK 293T细胞的方法,且简便易行. 相似文献
73.
Dot enzyme-linked immunosorbent assay (dot-ELISA), indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards ("marker") so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA. 相似文献
74.
天然热释光技术在海洋油气田勘查中的应用 总被引:18,自引:2,他引:18
沉积物热释光能有效地反映海洋环境下深部油气田引起的微弱放射性异常。热释光在不同的测试粒级、不同的岩性中的丰度不同,结果为粗粒级>细粒级、砂岩>粉砂质砂岩>泥质粉砂岩>泥岩。热释光也与部分金属元素含量有关,Ti、Ba、Zn、Fe^3 /Fe^2 与热释光呈正相关关系。热释光与烃类也呈较强的正相关性。不同采用深度热释光测量结果表明,热释光剂量随沉积物的沉积深度和沉积时间的增加而增加,深层样的热释光对油气藏的指示效果更好。 相似文献
75.
苏北盐城凹陷复合含油气系统 总被引:1,自引:0,他引:1
苏北盐城凹陷含油气系统可划分为上、下2个复合含油气系统。上复合含油气系统,即Pz、K2p、K2t(生)-K2t、E1f1(储)一E1f1 E1f2(盖),古生界、中生界浦口组和泰州组为烃源岩层,泰州组和阜宁组一段为储集层,阜宁组一段顶部至二段暗色泥岩为区域盖层,油气沿深大断裂运移,油气成藏关键时刻在古近纪晚期至新近纪早期,有利勘探区在深大断裂附近的中、新生界发育地区;下复合含油气系统,即Pz、K2p(生)-Pz、K2p(储)-K2p3 K2p4(盖),油气通过断层或不整合运移,油气大量生成的关键时刻为古近纪早期,该系统中气藏分布在古生界被中生界覆盖地区。 相似文献
76.
77.
用小麦岛和团岛观测站提供的青岛近海气象和水温资料,以适于SO2气体和硫酸盐(SO42-)气溶胶的干沉降模型研究了模型中的输送阻尼对两种污染物质干沉降速率的贡献,并计算了2003年青岛近海地区两种污染物质干沉降速率的季节变化。结果表明:对SO2而言,粘性副层传输系数对沉降速率的贡献大于空气动力学传输系数;对于SO42-,空气动力学传输系数对沉降速率的贡献要大于表面传输系数。SO2和SO42-的干沉降速率变化范围分别为0.187~0.868cm/s和0.188~0.532cm/s。两种污染物质干沉降速率的四季变化有相似的规律,即冬季>秋季>春季>夏季。 相似文献
78.
1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件,冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明:(1)水下地形主要为下泄流或上溯流控制的“深槽-槽沟-浅滩-湾口”的动力地貌体系,反映了河口湾“东进西出”的水流格局;(2)整个河口湾以淤积为主,只有崖门深槽有较明显的优势冲刷特征,并随着崖门深槽向海推移和河口湾“东进西出”水动力作用,黄茅海落潮三角洲相应向西南进积;(3)应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律,同样反映了河口湾具有“东进西出”的运移趋势。 相似文献
79.
Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis 总被引:3,自引:1,他引:3
Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po 相似文献
80.
Sequence variation of the first internal transcribed spacer of ribosomal DNA ( ITS - 1 ) was examined and its application to the study of genetic variation was explored in four populations of farter' s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly. 相似文献