用改进的超声波-溶菌酶法制备钝顶螺旋藻原生质球

用改进的超声波-溶菌酶法制备钝顶螺旋藻原生质球。钝顶螺旋藻(Spirulina platensis)经超声波破碎后,用1.5 mol/L NaCl的Zarrouk培养基洗去细胞外壁的胶质鞘,然后进行酶解。酶解条件为:pH7.2的0.2 mol/L磷酸钾缓冲液,0.8 mol/L KCl,0.5%溶菌酶,28~30℃水浴震荡酶解5~7 h,获得40%以上有活力的钝顶螺旋藻原生质球。     

青岛液体化工码头苯罐泄漏对大气环境的影响

预测和评价了青岛港液体化工码头苯贮罐苯泄漏对大气环境的影响．结果表明，苯罐一旦发生泄苯事故，会对大气环境造成不同程度的影响；在最不利天气条件下，泄漏挥发量为3375kg，会导致22人死亡；泄漏挥发量为1237kg，会导致4人死亡；如果同样的泄苯挥发量，发生在微风条件下，则分别有4人死亡和无人死亡；若发生33kg泄漏挥发量，无论在何种天气条件下，都不会导致人员伤亡。     

Effect of Cyclic Loading Frequency on Undrained Behaviors of Undisturbed Marine Clay

Based on a series of cyclic triaxial tests, the effect of cyclic frequency on the undrained behaviors of undisturbed marine clay is investigated. For a given dynamic stress ratio, the accumulated pore water pressure and dynamic strain increase with the number of cycles. There exists a threshold value for beth the accumulated pore water pressure and dynamic strain, below which the effect of cyclic frequency is very small, but above which the accumulated pore water pressure and dynamic strain increase intensely with the decrease of cyclic frequency for a given number of cycles. The dynamic strength increases with the increase of cyclic frequency, whereas the effect of cyclic frequency on it gradually diminishes to zero when the number of cycles is large enough, and the dynamic strengths at different frequencies tend to the same limiting minimum dynamic strength. The test results demonstrate that the reasons for the frequency effect on the undrained soil behaviors are beth the creep effect induced by the loading rate and the decrease of sample effective confining pressure caused by the accumulated pore water pressure.     

1998年春深圳湾环节环沟藻赤潮及其发生原因的探讨

1998年4月23日至29日深圳湾发生了较大规模的环节环沟藻赤潮,面积逾200km2,细胞密度高达3.8×106 个/L。赤潮发生于大量降雨之后 ,高峰期水体盐度在20以下。在深圳河河口海域 ,虽然富营养化程度也较高 ,但由于悬浮物含量过高 ,浮游植物及环节环沟藻数量较少。赤潮期间 ,深圳湾没有发生死鱼现象 ,养殖牡蛎的大量死亡估计与赤潮无关     

Wave Decay and Its Characteristics in Surf Zone

In accordance with the similarity between breaking waves and hydraulic jumps, the expressions for estimating wave decay and wave energy dissipation in the surf zone are derived based on the fundamental equations of fluid mechanics. Using the numerical solution of cnoidal wave theory, the various kinematic properties of waves in the surf zone, including the relative wave crest height, wave energy, and radiation stress are discussed. The values calculated with the method proposed in this paper are in good agreement with the experimental data gained by other researchers. The present expressions can be used in the studies of sediment transport on gently sloping beaches, especially on muddy beaches.     

A New Pursuit Mode of Nonlinear Wave Surface

The numerical mode of nonlinear wave transformation based on both the Laplace equation for water field and the Bemoulli equation for water surface is a kind of time-domain boundary problem with initial conditions. And the basis for establishing the numerical mode of nonlinear wave in time domain is to trace the position of wave free surface and to calculale the instantaneous surface height and surface potential function. This paper firstly utilizes the ‘0-1‘ combined BEM to separate the boundary by means of discretization of Green‘ s integral equation based on the Laplace equation, then separates the free surface of wave with FEM and derives the FEM equation of wave surface that satisfies the nonlinear boundary conditions. By jointly solving the above BEM and FEM equations, the wave potential and surface height could be obtained with iteration in time domain. Thus a new kind of nonlinear numerical mode is established for calculating wave transformation. The wave test in the numerical wave tank shows that the numerical simulation with this mode is of high accuracy.     

Characteristics of Scour and Deposition in Front of Breakwaters Under Irregular Broken Clapotis

First the scour and deposition patterns of the sandy seabed in front of a vertical breakwater under the action of irregular broken clapotis are investigated experimentally and classified into five types: scour type Ⅰ , scour type Ⅱ, scour type Ⅲ, deposition type Ⅰ, and deposition type Ⅱ . Secondly, the processes of formation of scour and deposition patterns are probed in comparison with those induced by regular broken clapotis and standing waves. Thirdly, the criteria for distinguishing scour and deposition patterns under irregular broken clapotis are presented.     

Modeling of Multi-Freedom Ship Motions in Irregular Waves with Fuzzy Neural Networks

1 .IntroductionAshiptravelingatseaundergoesundesirablewave inducedmotions ,namely ,surge ,sway ,heav ing ,rolling ,pitchingandyaw .Thesemotionsoftencauseproblemstothecrew ,theonboardequip mentand ,intheworstcase ,thesafetyofthevessel.Tominimizethewave inducedshipmotions ,controlsystemsmaybeapplied .Theaccuratemodelingofshipmotionsisthereforeveryimportantforshipdesignanddesignofmotioncontrolsystems .Manyresearchershavedevelopedshipmotionpredictionmethodsbasedonthepotentialflowtheo ries (Dong ,…     

A New Probability of Detection Model for Updating Crack Distribution of Offshore Structures

1 .IntroductionNondestructiveinspection (NDI)isveryimportantforensuringthereliabilityofoffshorestructuresintheirservicelives (Lauraetal.,1 996 ) .Itiswellknownthatdetectionofflawsinvolvesconsider ablestatisticaluncertainties.Asaresult,theprobabilityofdetection (POD)forallflawsofagivensizehasbeenusedintheliteraturetodefinethecapabilityofaparticularNDItechniqueinagivenen vironment.SincethedataofPODusuallyscatterlargely ,itisdifficulttodeterminewhichmodelfitstheavailabledatabest.Thismodelun…     

Action of Cnoidal Waves on Vertical Walls

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves