南黄海盆地南五凹阜宁组“广湖咸水”烃源岩及其油气勘探意义

通过对南五凹钻井所揭示的阜宁组进行岩性、地球化学、古生物及地震相等综合分析证实,南五凹阜宁组发育中深湖相泥质烃源岩,且该套烃源岩形成于"广湖咸水"沉积环境。"广湖"即湖盆分布广泛,中深湖相泥质烃源岩在凹陷深洼处和缓坡带均有发育,平面上面积大,纵向上厚度大,具备生成油气的物质基础。"咸水"即烃源岩形成时期湖盆受到了海侵影响,致使水体性质偏咸。南五凹与中国东部古新世—始新世期间曾遭受海侵影响的其他湖盆类似,其形成的咸化烃源岩具有"排烃早"的特征。南五凹阜宁组湖相烃源岩"广湖咸水"沉积特征对于油气勘探具有重要意义。     

中国南海极端风暴海况下固定式平台结构可靠性分析

基于中国南海海域风暴环境条件,研究分析南海海域固定式导管架平台结构整体性和可靠性,以及新建固定式平台结构设计准则。采用海洋环境数据后报方法,得到南海海域1972~2011共40年的风、浪、流联合数据,从中抽取风暴环境条件;利用通用荷载模型,将40年间的风暴环境数据转变成结构的荷载数据,即基底剪力或倾覆力矩;并计算得到风暴环境荷载的短期及长期分布,以及任意风暴下荷载的概率分布;根据结构可靠性模型,结合荷载的长期分布,研究基于结构暴露等级及失效概率的固定式平台结构强度储备比。根据计算,得到了不同暴露等级下中国南海平台的强度储备比,并与墨西哥湾及北海海域进行了比较,为新建平台提供设计参考。     

莱州湾东岸三山岛段砂质海岸沉积物运移动力机制

通过对三山岛岸段冬夏重复的地形测量和表层沉积物的粒度分析,研究了其地貌特征与地形变化规律、沉积物类型、粒度特征、运移趋势,并探讨了沉积物运移的动力机制。结果表明:该岸段可根据1985黄海高程-1.2m(低潮水位)和-6.5m(闭合深度)平面划分为海滩、水下岸坡和浅海陆架三个地貌单元,各地貌单元表层沉积物分布规律与地形变化特征区别显著。其中水下岸坡和浅海陆架地貌单元主要受潮流作用,海滩地貌单元主要受波浪作用。在西向落潮流和西南向潮余流的作用下,水下岸坡地貌单元发育一个中等规模潮流通道-沙脊沉积系统。表层沉积物以向西运移的趋势为主,少数滞留于西部潮流沙脊处,与地形"东侵西淤"的变化规律相符,这些西向运移的沉积物最终离开研究区,补给莱州浅滩。     

Evaluation of the biological toxicity of fluorine in Antarctic krill

Antarctic krill is a potentially nutritious food source for humans, but fluorine(F) toxicity is a matter of concern. To evaluate the toxicity of F in Antarctic krill, 30 Wistar rats were divided into three groups with different dietary regimens: a control group, a krill treatment group(150 mg·kg-1 F), and a sodium fluoride(NaF) treatment group(150 mg·kg-1 F). After three months, F concentrations in feces, plasma, and bone were determined, and the degree of dental and skeletal fluorosis was assessed. The F concentrations in plasma and bone from the krill treatment group were 0.167 0±0.020 4 mg.L-1 and 2 709.8±301.9 mg·kg-1, respectively, compared with 0.043 8±0.005 5 mg·L-1 and 442.4±60.7 mg·kg-1, respectively, in samples from the control group. Concentrations of F in plasma and bone in the krill treatment group were higher than in the control group, but lower than in the NaF treatment group. The degree of dental fluorosis in the krill treatment group was moderate, compared with severe in the NaF treatment group and normal in the control group. The degree of skeletal fluorosis did not change significantly in any group. These results showed that the toxicity of F in Antarctic krill was lower than for an equivalent concentration of F in NaF, but it was toxic for rats consuming krill in large quantities. To conclude, we discuss possible reasons for the reduced toxicity of F in Antarctic krill. The present study provides a direct toxicological reference for the consideration of Antarctic krill for human consumption.     

Identification of Fucans from Four Species of Sea Cucumber by High Temperature ~1H NMR

Acidic polysaccharide, which has various biological activities, is one of the most important components of sea cucumber. In the present study, crude polysaccharide was extracted from four species of sea cucumber from three different geographical zones, Pearsonothuria graeffei（Pg） from Indo-Pacific, Holothuria vagabunda（Hv） from Norwegian Coast, Stichopus tremulu（St） from Western Indian Ocean, and Isostichopus badionotu（Ib） from Western Atlantic. The polysaccharide extract was separated and purified with a cellulose DEAE anion-exchange column to obtain corresponding sea cucumber fucans（SC-Fucs）. The chemical property of these SC-Fucs, including molecular weight, monosaccharide composition and sulfate content, was determined. Their structure was compared simply with fourier infrared spectrum analyzer and identified with high temperature 1H nuclear magnetic resonance spectrum analyzer（NMR） and room temperature 13 C NMR. The results indicated that Fuc-Pg obtained from the torrid zone mainly contained 2,4-O-disulfated and non-sulfated fucose residue, whereas Fuc-Ib from the temperate zone contained non-, 2-O- and 2,4-O-disulfated fucose residue; Fuc-St from the frigid zone and Fuc-Hv from the torrid zone contained mainly non-sulfated fucose residue. The proton of SC-Fucs was better resolved via high temperature 1H NMR than via room temperature 1H NMR. The fingerprint of sea cucumber in different sea regions was established based on the index of anomer hydrogen signal in SC-Fucs. Further work will help to understand whether there exists a close relationship between the geographical area of sea cucumber and the sulfation pattern of SC-Fucs.     

我国西部1:50000地图新产品设计与表达技术

西部测图工程以"丰富产品种类,拓展服务领域"为设计宗旨,力求地图新产品开发,在传统4D产品的基础上,根据西部地物地貌特征补充了地形图要素内容,开发研制了影像地图、晕渲地形图新产品。本文针对原有地形图在表达西部地物地貌特征方面的不足,介绍了西部测图工程中地图新产品的内容设计与制图表达,包括地形图要素扩充内容与制图表达、影像地图产品内容设计与制图表达、晕渲地形图产品内容设计与制图表达等技术内容。地图新产品设计与表达技术已应用在西部测图中,指导生产了我国西部首批1:50000地形图、影像地图和晕渲地形图产品,丰富了我国基本比例尺地形图产品种类。     

邵阳地区闪电活动特征及其与降水的关系

利用闪电定位资料和常规观测站24 h的降水资料,对邵阳地区2008-2010年的闪电特征进行分析,并进一步探讨了邵阳地区闪电与降水的关系。结果表明：邵阳地区闪电多以负地闪为主,地闪频次的最大值中心位于东北部,呈东北-西南减少的趋势;地闪频次存在明显的日变化特征,闪电集中发生在13-19时;有闪电时,降水量不超过1 mm和10 mm的降水个例分别为29.63 %,76.30%;无闪电时,其值分别为69.44 %,96.30 %;超过20 mm的降水大多伴随有闪电,无闪电时发生概率极小;地闪频次与闪电对流降水的拟合系数优于其与所有降水的拟合系数,且随着统计尺度的增大,地闪与闪电对流降水的相关性变强。     

2010—2011年东海藻华高发区水体层化对东海原甲藻(Prorocentrum donghaiense Lu)藻华的影响

本文通过2010年和2011年不同时空角度对东海原甲藻藻华进行调查,研究水体层化对藻华发生和发展的影响。结果显示,起初东海原甲藻在50m等深线附近的外海繁殖与聚集,然后随着台湾暖流与长江冲淡水的相互作用,东海区域水体层化现象加剧,其细胞丰度越来越大,直至藻华发生并持续推进到近岸。大部分东海原甲藻细胞位于层化水体上方,而且当水体层化现象明显时,藻华的发展最为迅速且呈大规模爆发状态。结果还显示当出现叶绿素高值层时,总是伴随出现温盐突变现象。本项研究不仅第一次从时间角度记录了东海原甲藻藻华的发展动态,而且还发现了东海水体层化现象为东海原甲藻藻华的发生和发展提供了多种环境因子条件,这为该藻华的监控和预测提供了重要的科学依据。     

养殖方式、光照强度对哲罗鱼稚鱼生长与存活的影响

采用生态学方法,研究了养殖方式、光照强度对哲罗鱼稚鱼生长与存活的影响。结果表明:在养殖方式实验中,哲罗鱼稚鱼体长、体质量均以室内静水组为最高,室内流水组其次,室外流水组最低。存活率以室内流水组为最高,室外流水组次之,室内静水组最低。统计分析表明养殖方式对哲罗鱼稚鱼终末体长、体质量、特定生长率、体长生长参数以及体长、体质量变异系数的影响均达到极显著水平(P0.01)。在光照实验中,哲罗鱼稚鱼的存活率、体长、体质量及特定生长率、体长生长参数均随光照强度的增加而增加,说明在哲罗鱼稚鱼室内培育阶段,适当地增加部分光照有利于其存活、摄食与生长。本研究结果为哲罗鱼高效养殖提供了科学依据。     

三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因克隆及其表达分析

本研究克隆了三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因,命名为Pt CLIC。该基因c DNA全长2000bp,5’和3’非编码区(UTR)长分别为76bp和1162bp,开放阅读框长762bp,推测编码253个氨基酸,预测分子量为29.2k Da,理论等电点为5.93。生物信息学分析表明,Pt CLIC基因中未发现跨膜结构域,属于不稳定蛋白;同源性分析表明,Pt CLIC基因编码的氨基酸序列与蚤状溞(Daphnia pulex)CLIC基因的同源性高达83%;系统进化分析表明,三疣梭子蟹与拟穴青蟹首先聚为一支;实时荧光定量RT-PCR分析表明,Pt CLIC基因在选取的所有组织中均有表达,在肝胰腺中的相对表达量最高,且显著高于其它组织(P0.05)。低盐度胁迫显著改变了Pt CLIC基因在三疣梭子蟹鳃和肝胰腺中的表达模式,整体呈先上调后下调的趋势,并发现该基因在低盐耐受和低盐敏感家系中的表达规律差异显著。研究结果表明Pt CLIC基因在三疣梭子蟹渗透压调节中发挥重要作用,可辅助三疣梭子蟹耐低盐品系的选育。