Gaussian decomposition and component pigment spectral analysis of phytoplankton absorption spectra

The absorption spectrum of phytoplankton is an important bio-optical parameter for ocean color hyperspectral remote sensing;its magnitude and shape can be affected considerably by pigment composition and concentration.We conducted Gaussian decomposition to the absorption spectra of phytoplankton pigment and studied the spectral components of the phytoplankton,in which the package effect was investigated using pigment concentration data and phytoplankton absorption spectra.The decomposition results were compared with the corresponding concentrations of the five main pigment groups(chlorophylls a,b,and c,photo-synthetic carotenoids(PSC),and photo-protective carotenoids(PPC)).The results indicate that the majority of residual errors in the Gaussian decomposition are0.001 m~(-1),and R~2 of the power regression between characteristic bands and HPLC pigment concentrations(except for chlorophyll b)was0.65 or greater for surface water samples at autumn cruise.In addition,we determined a strong predictive capability for chlorophylls a,c,PPC,and PSC.We also tested the estimation of pigment concentrations from the empirical specific absorption coefficient of pigment composition.The empirical decomposition showed that the Ficek model was the closest to the original spectra with the smallest residual errors.The pigment decomposition results and HPLC measurements of pigment concentration are in a high consistency as the scatter plots are distributed largely near the 1:1 line in spite of prominent seasonal variations.The Wozniak model showed a better fit than the Ficek model for Chla,and the median relative error was small.The pigment component information estimated from the phytoplankton absorption spectra can help better remote sensing of hyperspectral ocean color that related to the changes in phytoplankton communities and varieties.     

Morphological and molecular diagnoses of Polydora brevipalpa Zachs,1933(Annelida: Spionidae) from the shellfish along the coast of China

Shell-boring species Polydora brevipalpa Zachs, 1933 is redescribed based on morphological observations and molecular approach for future unambiguous identification. Genetic distance analyses showed that the interspecific polydorid variation(16.7%–25.6%) was at least 15 times higher than the intraspecific one(0.2%–0.9%) based on the cytochrome c oxidase subunit I(CO1) gene sequences of polydorids. However, 18 S rDNA variation pattern demonstrated a rather narrow barcoding gap, with the interspecific polydorid variation(0.5%–5.6%) being very close to the intraspecific one(0.0%–0.4%). As such, the CO1 gene exhibited better DNA barcode for identification of polydorids than the 18 S rDNA gene because of the su ciently large barcoding gaps. Analysis of molecular variance results based on CO1 gene sequences showed that most variations in sequences(97.79%) lay within groups of adult worms and egg capsules rather than between them. This indicated that egg capsules from Crassostrea gigas(Thunberg,1793) in Ningbo and Nantong were related to the adult worms from Patinopecten yessoensis(Jay, 1857) in Dalian, and both of them belonged to P. brevipalpa. This result was further supported by parsimony network analysis, which showed that egg capsules collected from dif ferent localities and adult worms shared a single haplotype. This study was the first to report both P. brevipalpa infestation on C. gigas and to utilise the known CO1 sequences of the adult polydorids to validate morphologically unidentified egg capsules or early larvae. P. brevipalpa was most possibly brought to Chinese waters through transportation of Pa. yessoensis brood stock from Japan.     

Analyses of egg size,otolith shape,and growth revealed two components of small yellow croaker in Haizhou Bay spawning stock

The geographic al variations in life history characteristic s of small yellow cro aker Larimich thys polyactis,caused by experienced different environmental conditions,have been observed in China seas.Previous studies based on spatial distribution,migration route,and body morphometrics suggested a complex stock structure.In this study,to clarify the source of a spawning stock,we investigated the reproduction strategy and inter-structure of the Haizhou Bay(HZB)spawning stock in the middle Yellow Sea from both egg survey and adult otolith increment analysis.Egg and adult samples were collected from three surveys during spawning season in 2013.Distinct spatial and temporal variations were detected in egg distribution and size,and otolith shape analy sis of adult fishes revealed two morphotypes with different increment growth using random forest cluster.The results indicate the existence of two components within the same spawning stock in HZB from different wintering grounds,and accordingly special protection should be required for this stock given the significance to maintain connectivity between adj acent subpopulations.     

拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析

本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。     

基于F型mtDNA D-Loop的厚壳贻贝(Mytilus coruscus)群体遗传多样性研究

为研究东海区厚壳贻贝(Mytilus coruscus)遗传多样性,以厚壳贻贝F mtDNA D-loop为标记,对浙江省舟山嵊山岛、宁波渔山岛、温州南麂岛、福建省宁德湾和莆田南日岛五个海区的厚壳贻贝群体进行了遗传分析。结果表明,厚壳贻贝各群体的遗传多样性差异不明显,在5个群体中,宁德群体的遗传多样性相对最丰富;将5个群体作为一个整体时,呈现出较高的单倍型多样性和较低的核苷酸多样性。对厚壳贻贝5个群体间的遗传分化系数(Fst)和基因流(Nm)进行检测,结果显示群体间Fst值都很低,但Nm值都很高(Nm绝对值1),表明5个群体间存在丰富的基因交流。但宁德群体与浙江沿海的3个群体(嵊山、渔山、温州)的Fst值相对较高,且差异显著(P0.5),表明宁德群体与这3个群体间出现遗传分化。本研究旨在为海洋经济贝类资源的保护管理提供理论依据。     

Spatio-temporal variability of small fishes related with environmental factors in a typical domestic tap water lake,Eastern China

The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small ?sh community in Kuilei Lake(China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into ?ve major habitats:(1) macrophyte covered shallow habitat of water depth< 2.00 m,(2) uncovered or less-covered shallow habitat(2.00 m–3.50 m),(3) uncovered medium shallow habitat(3.50 m–5.00 m),(4) uncovered medium deep habitat(5.00 m–6.50 m) and(5) uncovered deep habitat(6.50 m–8.50 m). The abundance and composition of small ?sh were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance(accounted for 88.96% of the total) and occurrence(more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus,Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small ?sh species. The results of correlation analysis identi?ed that species richness( Sr), Shannon-Wiener diversity index( H′)and Margalef′s richness index( D) were signi?cantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis(RDA) revealed that the spatial distributions of most small ?shes were negatively associated with water depth. The details of these ?ndings are bene?cial to understanding the adaptation of the small ?shes in degraded environments, and to developing suitable biomanipulation strategies for the management of ?sh resources and water quality in the lakes along the lower reach of the Changjiang(Yangtze) River basin.     

Dynamic metabolite alterations of Portunus trituberculatus during larval development

A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.     

高分辨率光学遥感场景分类的深度度量学习方法

针对高分辨率光学遥感影像场景具有同类型内部差异大、不同类型间相似度高导致部分场景识别困难的问题,本文提出了一种深度度量学习方法。首先在深度学习模型的特征输出层上为每类预设聚类中心,其次基于欧氏距离方法设计均值中心度量损失项,最后联合交叉熵损失项以及权重与偏置正则项构成模型的损失函数。该方法的目标是在特征空间上使同类型特征聚集并扩大类型间的距离以提高分类准确率。试验结果表明,本文方法有效地提升了分类准确率。在RSSCN7、UC Merced和NWPU-RESISC45数据集上,与现有方法相比,分类准确率分别提高了1.46%、1.09%和2.51%。     

我国自然资源、自然资源资产监测发展现状及问题分析

自然资源、自然资源资产监测是实现自然资源统一管理和确权的基础性工作。本文通过对自然资源、自然资源资产监测概念和内涵的研究,从行政监测、航天监测、航空监测、地表监测、地下矿产监测、水资源监测、海洋监测等角度介绍了我国自然资源监测研究及工作实践的方法和手段;并对科学界定自然资源内涵与分类及监测目录、国家自然资源监测网络整合与建设、自然资源监测时空框架构建等主要问题的解决提出了建议。     

基于PS-InSAR技术的深圳北站表面位移监测研究

利用2015-01-2018-08的40景COSMO-SKyMed HIMAGE雷达影像,采用永久散射体合成孔径雷达干涉测量(permanent scatterers interferometric synthetic aperture radar,PS-InSAR)技术获得了深圳北站时序形变信息,并基于GIS空间分析...