拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析

本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。     

基于F型mtDNA D-Loop的厚壳贻贝(Mytilus coruscus)群体遗传多样性研究

为研究东海区厚壳贻贝(Mytilus coruscus)遗传多样性,以厚壳贻贝F mtDNA D-loop为标记,对浙江省舟山嵊山岛、宁波渔山岛、温州南麂岛、福建省宁德湾和莆田南日岛五个海区的厚壳贻贝群体进行了遗传分析。结果表明,厚壳贻贝各群体的遗传多样性差异不明显,在5个群体中,宁德群体的遗传多样性相对最丰富;将5个群体作为一个整体时,呈现出较高的单倍型多样性和较低的核苷酸多样性。对厚壳贻贝5个群体间的遗传分化系数(Fst)和基因流(Nm)进行检测,结果显示群体间Fst值都很低,但Nm值都很高(Nm绝对值1),表明5个群体间存在丰富的基因交流。但宁德群体与浙江沿海的3个群体(嵊山、渔山、温州)的Fst值相对较高,且差异显著(P0.5),表明宁德群体与这3个群体间出现遗传分化。本研究旨在为海洋经济贝类资源的保护管理提供理论依据。     

Morphological and molecular diagnoses of Polydora brevipalpa Zachs,1933(Annelida: Spionidae) from the shellfish along the coast of China

Shell-boring species Polydora brevipalpa Zachs, 1933 is redescribed based on morphological observations and molecular approach for future unambiguous identification. Genetic distance analyses showed that the interspecific polydorid variation(16.7%–25.6%) was at least 15 times higher than the intraspecific one(0.2%–0.9%) based on the cytochrome c oxidase subunit I(CO1) gene sequences of polydorids. However, 18 S rDNA variation pattern demonstrated a rather narrow barcoding gap, with the interspecific polydorid variation(0.5%–5.6%) being very close to the intraspecific one(0.0%–0.4%). As such, the CO1 gene exhibited better DNA barcode for identification of polydorids than the 18 S rDNA gene because of the su ciently large barcoding gaps. Analysis of molecular variance results based on CO1 gene sequences showed that most variations in sequences(97.79%) lay within groups of adult worms and egg capsules rather than between them. This indicated that egg capsules from Crassostrea gigas(Thunberg,1793) in Ningbo and Nantong were related to the adult worms from Patinopecten yessoensis(Jay, 1857) in Dalian, and both of them belonged to P. brevipalpa. This result was further supported by parsimony network analysis, which showed that egg capsules collected from dif ferent localities and adult worms shared a single haplotype. This study was the first to report both P. brevipalpa infestation on C. gigas and to utilise the known CO1 sequences of the adult polydorids to validate morphologically unidentified egg capsules or early larvae. P. brevipalpa was most possibly brought to Chinese waters through transportation of Pa. yessoensis brood stock from Japan.     

Analyses of egg size,otolith shape,and growth revealed two components of small yellow croaker in Haizhou Bay spawning stock

The geographic al variations in life history characteristic s of small yellow cro aker Larimich thys polyactis,caused by experienced different environmental conditions,have been observed in China seas.Previous studies based on spatial distribution,migration route,and body morphometrics suggested a complex stock structure.In this study,to clarify the source of a spawning stock,we investigated the reproduction strategy and inter-structure of the Haizhou Bay(HZB)spawning stock in the middle Yellow Sea from both egg survey and adult otolith increment analysis.Egg and adult samples were collected from three surveys during spawning season in 2013.Distinct spatial and temporal variations were detected in egg distribution and size,and otolith shape analy sis of adult fishes revealed two morphotypes with different increment growth using random forest cluster.The results indicate the existence of two components within the same spawning stock in HZB from different wintering grounds,and accordingly special protection should be required for this stock given the significance to maintain connectivity between adj acent subpopulations.     

Gaussian decomposition and component pigment spectral analysis of phytoplankton absorption spectra

The absorption spectrum of phytoplankton is an important bio-optical parameter for ocean color hyperspectral remote sensing;its magnitude and shape can be affected considerably by pigment composition and concentration.We conducted Gaussian decomposition to the absorption spectra of phytoplankton pigment and studied the spectral components of the phytoplankton,in which the package effect was investigated using pigment concentration data and phytoplankton absorption spectra.The decomposition results were compared with the corresponding concentrations of the five main pigment groups(chlorophylls a,b,and c,photo-synthetic carotenoids(PSC),and photo-protective carotenoids(PPC)).The results indicate that the majority of residual errors in the Gaussian decomposition are0.001 m~(-1),and R~2 of the power regression between characteristic bands and HPLC pigment concentrations(except for chlorophyll b)was0.65 or greater for surface water samples at autumn cruise.In addition,we determined a strong predictive capability for chlorophylls a,c,PPC,and PSC.We also tested the estimation of pigment concentrations from the empirical specific absorption coefficient of pigment composition.The empirical decomposition showed that the Ficek model was the closest to the original spectra with the smallest residual errors.The pigment decomposition results and HPLC measurements of pigment concentration are in a high consistency as the scatter plots are distributed largely near the 1:1 line in spite of prominent seasonal variations.The Wozniak model showed a better fit than the Ficek model for Chla,and the median relative error was small.The pigment component information estimated from the phytoplankton absorption spectra can help better remote sensing of hyperspectral ocean color that related to the changes in phytoplankton communities and varieties.     

基于长时间序列遥感数据的阿伯德尔国家公园 生态环境质量评价

阿伯德尔国家公园位于号称肯尼亚“水塔”的阿伯德尔山脉上,是肯尼亚重要的旅游景区和珍稀动植物保护区,开展该地生态环境质量评价对肯尼亚水资源保护与可持续发展具有重要的意义。本研究基于1987-2018年的Landsat系列遥感数据,通过计算综合遥感生态指数RSEI,结合趋势线分析法对阿伯德尔国家公园近32年间生态环境质量变化进行客观定量分析。研究结果显示：① 1987-2018年研究区RSEI均值从0.62下降到0.51,生态环境质量退化的区域面积为551.52 km ²,占总面积的71.85%,表明在气候变化和人类活动影响下,1987-2018年研究区的环境状况呈恶化趋势;②环境质量显著恶化的区域主要分布于亚高山植被区和东部低海拔森林覆盖区,亚高山地区植被生态系统结构单一,稳定性差,对气候变化和人类活动更为敏感;而东部区域存在过度开发,非法砍伐森林等现象,且旅游开发强度大,环境恶化与人类活动密切相关。     

GPS/PWV时间序列特征提取方法的研究

针对GAMIT/GLOBK软件解算得到的4 a GPS/PWV时间序列的特征提取问题,提出基于滤波辅助的部分集成经验模态分解（PEEMD）与Hilbert谱分析相结合的特征提取方法。首先,在PEEMD方法的基础上,结合滤波辅助的PEEMD方法与Hilbert谱分析,建立GPS/PWV时间序列特征提取模型;然后,将所提出的方法应用于TNML测站4 a的GPS/PWV长时间序列和7 d的GPS/PWV短时间序列分析中,并将滤波辅助的PEEMD结果与传统的小波分解结果进行对比。结果表明,该特征提取方法能准确有效地提取出 GPS/PWV时间序列中的周年周期和日周期特征分量,滤波辅助的PEEMD分解结果与小波分解结果一致,且提取的特征分量与原始信号更加吻合。     

基于PS-InSAR技术的深圳北站表面位移监测研究

利用2015-01-2018-08的40景COSMO-SKyMed HIMAGE雷达影像,采用永久散射体合成孔径雷达干涉测量(permanent scatterers interferometric synthetic aperture radar,PS-InSAR)技术获得了深圳北站时序形变信息,并基于GIS空间分析...     

东海三定点周日海流观测的准调和分析

应用短期资料的潮流准调和分析方法,对东海三定点测站获得的表、中、底3层的25h连续海流资料进行了分析,计算了3测站O1,K1,M2,S2,M4,MS4 6个主要分潮的北、东分量潮流调和常数,并给出了各测站在各层的潮流椭圆要素。计算结果表明：该海区潮流属于正规半日潮流性质,半日分潮流的北分量大于东分量,呈现旋转流的性质,按顺时针向旋转。分析结果也反映出表层、中层的浅水分潮在观测海流中所占的份额高于底层的浅水分潮。结果有助于了解该海区的潮流性质并为潮流数值模拟提供校验实测数据。通过与同期获得的悬浮物浓度剖面数据的比对,将有助于研究潮流与该地区悬浮泥沙浓度分布之间的关系。     

浒苔(Enteromorpha prolifera)生活史的初步研究

通过实验室培养实验,全面地观察描述了浒苔生活史各个阶段的发育情况。雌雄异体的配子体成熟后,放散双鞭毛的配子,雌雄配子结合成合子进而发育成孢子体,配子也可以进行单性生殖发育成膜状的配子体。孢子体成熟放散四鞭毛的游孢子,游孢子固着后,可直接发育成配子体。其生活史是单倍体的配子体与二倍体的孢子体相互交替的同形世代交替。雌雄配子具有正趋光性,易大量聚集,结合后同游孢子一样都呈负趋光性。