蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

Protective effect of topically applied polypeptide from Chlamys farreri against ultraviolet radiation-induced chronic skin damage in guinea pig

Polypeptide fromChlamys farreri (PCF), a topical polypeptide isolated fromChlamys farreri, was used in this experiment aimed to investigate the photoprotective effect of PCF against chronic skin damage induced by ultraviolet A (UVA) and ultraviolet B (UVB) radiation. The chronic ultraviolet-irradiated guinea pig model was established, and visible changes in the skin including wrinkling, sagging and erythema were observed. Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in the dorsal skin were determined using biochemical methods. The results showed: (1) PCF (5 % and 20%) could greatly protect the dorsal skin of guinea pig against wrinkling, sagging and erythema induced by UV radiation in a concentration-dependent manner. (2) PCF could reduce MDA formation in the dorsal skin caused by UV irradiation, while increasing the activities of SOD and GSH-px. (3) The differences among the PCF groups and UV model group were significant (P<0.05,P<0.01). These results indicated that topical application, of PCF provided broad solar UV spectrum photoprotection; and that the antioxidant property of PCF might play a role in photoprotection. Project No. 39970638 supported by the NSFC, and the Science and Technology Bureau of Qingdao (No. 2001-28-50).     

基于ARP协议的网络主机地址的识别技术研究

从移动IPv6工作原理入手，IP地址管理器是针对越来越严重的IP地址盗用现象而设计的一套有效的监控和管理IP地址的方案。分析与研究IP地址管理器中的信息收集模块后，以Winpcap为开发工具，利用ARP，附等协议完成对网络中活动主机的扫描以及信息（主机名、MAC、操作系统）的收集和识别。模块实现了对网络的自动实时监控，有效地提高了管理效率。     

胶东“玲珑-焦家式”金矿资源潜力与找矿

“玲珑焦家式”金矿的成岩成矿,从新太古代至中生代存在着继承性的成因关系,成矿具有长期性、多源性特点,但中生代燕山早期岩浆活动是主导成矿因素。区域化探成果显示胶东金矿直接矿源岩———郭家岭花岗岩金的丰度比原始矿源岩———早前寒武纪变质沉积岩系及侵入岩系金的丰度低1/2。郭家岭花岗岩面积在2 km2以上岩体定量估计金亏损总量与胶东金矿总量预测的资源量相当,从一个侧面展现了胶东金矿资源的潜力。望儿山金矿的找矿实践展示,焦家式金矿在1 000 m以下尚有巨大潜力,整个胶东金矿产区在3 000 m以上的资源潜力应在5000 t以上。     

差分法周跳探测与修复方法改进

在目前常用的周跳探测与修复方法基础上 ,提出了首先将观测资料按照观测历元不连续分成若干小弧段 ,然后利用差分法进行周跳探测 ,根据差分后周跳放大的特性判断周跳和野值 ,并确定其位置利用宽带组合和电离层组合的方法解算周跳大小。通过实例验证了其有效性。     

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …     

北秦岭晋宁期中酸性侵入岩带地球化学特征与构造环境

北秦岭侵入岩带中的中酸性侵入岩主要形成于晋宁旋回的中晚期阶段。主要岩石类型包括辉石闪长岩、闪长岩、石英闪长岩、英云闪长岩、花岗闪长岩、斜长花岗岩、花岗岩和钾长花岗岩。岩石化学、稀土元素和微量元素地球化学研究证明,这些岩石主要形成于B型俯冲和碰撞造山的构造环境中,而钾长花岗岩形成于后造山的伸展阶段。据此,该侵入岩带揭示了北秦岭褶皱带在晋宁旋回中晚期阶段的演化过程。     

CPLD技术及其在现代时统中的应用

对CPLD(复杂可编程逻辑器件)技术的基本特征和发展趋势作了简要介绍,揭示了该技术在现代数字系统中的重要地位及作用.利用CPLD对时统设备IRIG-B码产生器进行集成,其实验结果表明,集成了的B码产生器不但简单、可靠,而且便于调试,克服了以往硬件电路复杂的缺点.     

Geological environment conflicts of Kunming Basin and its urban sustainable development

Kunming Basin locates middle of Yunnan altiplano and has a particularity in geography,topographic and geological environment.With the urban dilation quickly,add the reason of the unreasonable city layout,conflicts between environment and urban resources consumption become shrill increasingly.It is human being activities that lead to vulnerability and depravation of geological environment in local.Take a few examples on geological environment to expatiate relationship between urban construction and geological environment carrying capacity,and find a way how to make a better plan for urban sustainable development to achieve new balance between man and nature in local.