固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.     

斜带髭鲷幼鱼生长和生态转换效率的研究

在实验室内, 22. 5±0. 5℃条件下以去头去骨去鳞片去皮的金色小沙丁鱼的纯肉糜为饵料饲养斜带髭鲷幼鱼,测定体重范围在 4. 6~6. 5g的斜带髭鲷的体重、体长的生长曲线及其生态转换效率和特定生长率.实验结果表明,斜带髭鲷的体重、体长在实验期间直线增长,体重、体长的生长曲线分别为Wt(w.w.湿重,下同 ) =0. 391 7t 5. 766 2(R=0. 999 6)和Lt=0. 105t 6. 265(R=0. 989 9).用胃含物法测定斜带髭鲷的胃排空率、日摄食量、日生长量、生态转换效率和特定生长率.实验得到斜带髭鲷消化道的瞬时排空率Rt= 1. 23 4×10-3 (m/m) /h,日摄食量Cd= 1. 342 9g(w.w. ),生态转换效率Eg=29. 50% ±2. 77% [m/m(w.w. ) ],特定生长率SGR=4. 71%±0. 69% [m/m(w.w. ) ].     

Action of Cnoidal Waves on Vertical Walls

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves     

Dynamic Response Analysis of Interaction System of R.C. Gravity Single-Leg Platform

In this paper, the responses of the interaction system of R.C. gravity single-leg platform to seismic excitation are mainly analysed. A set of nonlinear equations for the interaction system are established by using the wave, one is the soil-structure interaction and the other is the fluid-structure interaction. The seismic response of the interaction system is analysed for the influence of the asymmetric structure, fluid action, etc. with the input of seismic SH waves in any direction. The numerical results are given for a simple example.     

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.     

Fuzzy Fatigue Reliability Analysis of Offshore Platforms in Ice-Infested Waters

1 .IntroductionTheBohaiGulfisinfestedbyiceinthewholewinter,andoffshorestructuresthereexperiencenotonlywind ,waves ,currentsandearthquake ,butalsoiceloadswhicharecomplicatedbecauseofthedynamicinteractionbetweenicesheetsandstructures.Duanetal.( 2 0 0 0 )hadacomprehensivere viewofthelatestadvancesonice inducedvibration ,fatigueandfractureofoffshorestructures ,payingmuchattentiontothedifferencesinicebreakingmodeanddynamicsofstructuresindifferentice in festedwaters .Theypresentedcriticallythelates…     

南海南部NS93-5孔沉积物磁化率特征及其对全球气候变化的记录

对南海南部长533cm的NS－93孔连续采取423块样品，测量低场下的质量磁化率。并间隔取样202块，测量ARM。通过与沉积层序、氧同位素和矿物特征对比分析，我们认为NS－93孔磁化率的变化主要受控于外源磁性矿物浓度的变化，其值的高低与沉积物中碎屑矿物的含量相关，与碳酸盐的含量反相关，说明碳酸盐矿物对磁性矿物浓度具有稀释作用。在磁化率特征变化曲线上，与氧同位素阶段和Heinrich变冷事件、D－O旋回有好的对应关系。磁化率的相对高值与氧同位素奇数阶段和气候的暖期相对应，而相对低值则与气候冷期对应。这一规律反映了东亚季风、大洋环流强弱和陆源碎屑输入对沉积物磁化率的控制作用。同时，从ARM和磁化率的关系分析，南海南部在氧同位素阶段6沉积时期处于相对稳定的环境，南面的Borneo海峡可能是封闭的。     

珠江三角洲网河区水位变化趋势研究

根据珠江三角洲网河区29个验潮站的实测验潮记录,应用傅氏变换与最平滤波器串联的方法来消除月均序列的周期波动对确定水位变化趋势的影响,由低通序列一元线性回归分析确定各站水位的变化趋势;结果表明,周期波动对确定海平面变化趋势的影响是显著的.应用经验正交函数(EOF)对网河区的水位变化场进行分解,由相互独立的时间函数和空间特征函数表征网河区区域的水位变化特征;应用时间特征函数计算区域水位的平均变化率为0.02mm/a.根据验潮站的水位变化趋势,探讨网河区水位变化与河床冲淤的关系.     

Wave-Current Forces on Vertical Cylinders in Side-by-Side Arrangement for Irregular Waves Combined With Opposing Current

- The wave-current forces on vertical piles in side-by-side arrangement induced by irregular waves with opposing current are investigated experimentally in this paper. The characteristics in both time and frequency domain of in-line, lift and resultant forces are analyzed. The grouping effect coefficients of inline, lift and resultant forces on piles related to KC number and relative spacing parameters are given. These results are compared with those in the case of irregular waves combined with following currents. It is found that the results in these two cases are quite different. The range of KC number tested is 10- 60, the range of Reynolds number is (0.55-3.43) ×104.     

Test Study on Current Force on Mooring Ships

In respect of an offshore berthing pillar, the test study result of current force acting on mooring ships is described in this paper. Empirical and semi-empirical relationships of current force coefficient are given according to the angle between the flow direction and the ship's longitudinal axis, which are coincident with the result of theoretical analysis.