Isolation, characterization of Rhodococcus sp. P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons

Rhodococcus sp. P14 was isolated from crude oil-contaminated sediments. This strain was capable of utilizing three to five rings polycyclic aromatic hydrocarbons (PAHs) including phenanthrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) as a sole carbon and energy source. After cultivated with 50mg/L of each PAH, strain P14 removed 43% Phe, 34% Pyr and 30% BaP in 30 d. Four different hydroxyphenanthrene products derived from Phe by strain P14 (1,2,3,4-hydroxyphenanthrene) were detected using SPME-GC-MS. Strain P14 also was capable of degrading mineral oil with n-alkanes of C17 to C21 carbon chain length. Compared with glucose-grown cells, PAHs-grown cells had decreased contents of shorter-chain length fatty acids (≤ C16:0), increased contents of C18:0, Me-C19:0 and disappeared odd-number carbon chain fatty acids. The contents of unsaturated C19:1, Me-C19:0 increased and C18:0 decreased in mineral oil-grown cells. At the same time, the strain P14 tended to float when cultivated in mineral oil-supplemented liquid medium. The degradation capability of P14 to alkane and PAHs and its floating characteristics will be very helpful for future's application in oil-spill bioremediation.     

Organochlorine pesticides in sediments of Laizhou Bay and its adjacent rivers, North China

Organochlorine pesticides (HCHs, DDTs and HCB) have been investigated in sediments of Laizhou Bay and its adjacent rivers. The average concentrations of HCHs, DDTs and HCBs are 0.09 ng/g dw, 0.59 and 0.05 for marine sediments and 0.55, 8.6 and 1.1 for riverine sediments, respectively. Laizhou Bay is less contaminated by HCHs and DDTs in comparison with other coastal regions around the world, including the other two biggest bays in Bohai Sea. Fresh input of lindane and dicofol-type DDT were found in several riverine sites. Relatively strong TOC-dependence of HCB distribution in the entire studied area suggested no point source existed and/or water-sediment equilibrium achieved. No significant difference of α/γ-HCH ratios have been observed between marine and riverine sediments. However, o,p′-DDT/p,p′-DDT ratios varied largely between the two regions.     

Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes)

The effects of polychlorinated biphenyls (PCBs) on liver function and their differences between sexes were analyzed in Japanese medaka (Oryzias latipes) exposed to PCB126 or Kanechlor-400 (KC-400) using microarray. PCB exposure induced vitellogenin 1 expression in female medaka while suppressing choriogenin genes, which suggests that the effects of PCBs on estrogen-responsive genes do not occur directly through an estrogen receptor-mediated pathway. Reduction of androgen receptor alpha expression was also observed, and the gene expression pattern in PCB-exposed males changed to become more similar to that of females. Furthermore, changes in glycolysis-related genes indicate that PCB exposure might enhance glucose production via gluconeogenesis in the liver of medaka. Taken together, our results suggest that PCBs disrupt the endocrine system, especially androgen function, and may have the potential to cause demasculinizing effects. Additionally, induction of gluconeogenesis might be a response to maintain glucose levels consumed as a result of PCB exposures.     

基于地震折射剖面的菲律宾海板块15～20°N九州—帕劳海岭的地壳结构变化

得到了菲律宾海板块15～20°N之间4条垂直于九州—帕劳海岭(KPR)剖面的重合广角和多道地震反射数据。九州—帕劳海岭为晚始新世时期形成的残余岛弧,其下的地壳厚度沿走向由8km变至20km,并且总是大于西侧西菲律宾海盆和东侧帕里西维拉海盆的相邻洋壳厚度。4条剖面中地壳最厚的区域位于九州—帕劳海岭与冲之鸟礁接壤处,初步推断可能是下地壳增厚的缘故。没有明显的证据能证明,在伊豆—小笠原—马里亚纳岛弧共轭断裂对应部分之下有推测出的P波速度为6.0～6.5km/s的厚(>5km)中地壳层。我们的结果表明,15～20°N的九州—帕劳海岭之下的地壳,相对于报道有P波速度6.0km/s的中地壳层的更北部海岭地区而言,属于不太成熟的岛弧地壳。     

推断勒夫波相速度的圆形台阵微震新技术

介绍了简单而又新颖的公式,使用微震的二分量,即圆形台阵记录的水平运动直接推断勒夫波相速度(cL)时,这些公式具有帮助作用。我们的公式与一般用于推断瑞雷波相速度(cR)的微震探测技术的空间自相关(SPAC)方法的公式类似。虽然SPAC方法现有的理论确实提供了估计cL的可能性,但这只能通过求解非线性的方程组才能实现,而其中待求解的未知数也包括cR以及瑞雷波?勒夫波的幂分配比。相反,在我们公式中cL是出现的唯一未知数。基于我们推荐公式——我们称为SPAC+L、SPAC-L和CCA-L(其中CCA表示无中心圆形台阵)法——的cL估算法的现场适用范围通过2个测试点的分析结果说明。在所提出的3种技术中,SPAC+L法由于表现最佳而出名。3种方法的有效波长范围下限是台阵半径r的2～5倍区域,上限是10～25r的区域。近年来,提出了类似的仅涉及cL的圆形台阵微震技术,但与它们相比,我们在此给出的方法,不是在数理计算上繁冗更少,就是在数学上更为简单。     

Impacts of diverted freshwater on dissolved organic matter and microbial communities in Barataria Bay, Louisiana, U.S.A

Here we present results of an initial assessment of the impacts of a water diversion event on the concentrations and chemical composition of dissolved organic matter (DOM) and bacterioplankton community composition in Barataria Bay, Louisiana U.S.A, an important estuary within the Mississippi River Delta complex. Concentrations and spectral properties of DOM, as reflected by UV/visible absorbance and fluorescence, were strikingly similar at 26 sites sampled along transects near two western and two eastern areas of Barataria Bay in July and September 2010. In September 2010, dissolved organic carbon (DOC) was significantly higher (568.1-1043 μM C, x=755.6+/-117.7 μM C, n=14) than in July 2010 (249.1-577.1 μM C, x=383.7+/-98.31 μM C, n=14); conversely, Abs254 was consistently higher at every site in July (0.105-0.314) than in September (0.080-0.221), averaging 0.24±0.06 in July and 0.15±0.04 in September. Fluorescence data via the fluorescence index (FI450/500) revealed that only 30% (8 of 26) of the July samples had an FI450/500 above 1.36, compared to 96% (25 of 26) for the September samples. This indicates a more terrestrial origin for the July DOM. Bacterioplankton from eastern sites differed in composition from bacterioplankon in western sites in July. These differences appeared to result from reduced salinities caused by the freshwater diversion. Bacterioplankton communities in September differed from those in July, but no spatial structure was observed. Thus, the trends in bacterioplankton and DOM were likely due to changes in water masses (e.g., input of Mississippi River water in July and a return to estuarine waters in September). Discharge of water from the Davis Pond Freshwater Diversion (DPFD) through Barataria Bay may have partially mitigated some adverse effects of the oil spill, inasmuch as DOM is concerned.     

Geographical distribution of red and green Noctiluca scintillans

The dinoflagellate Noctiluca scintillans is one of the most important and abundant red tide organisms and it is distributed world-wide. It occurs in two forms. Red Noctiluca is heterotrophic and fills the role of one of the microzooplankton grazers in the foodweb. In contrast, green Noctiluca contains a photosynthetic symbiont Pedinomonas noctilucae (a prasinophyte), but it also feeds on other plankton when the food supply is abundant. In this review, we document the global distribution of these two forms and include the first maps of their global distribution. Red Noctiluca occurs widely in the temperate to sub-tropical coastal regions of the world. It occurs over a wide temperature range of about 10°C to 25°C and at higher salinities (generally not in estuaries). It is particularly abundant in high productivity areas such as upwelling or eutrophic areas where diatoms dominate since they are its preferred food source. Green Noctiluca is much more restricted to a temperature range of 25°C-30°C and mainly occurs in tropical waters of Southeast Asia, Bay of Bengal (east coast of India), in the eastern, western and northern Arabian Sea, the Red Sea, and recently it has become very abundant in the Gulf of Oman. Red and green Noctiluca do overlap in their distribution in the eastern, northern and western Arabian Sea with a seasonal shift from green Noctiluca in the cooler winter convective mixing, higher productivity season, to red Noctiluca in the more oligotrophic warmer summer season.     

P／T之交一个大量缺氧的分层洋的发育及其水体的短暂大规模混合作…

通过研究日本丹波地体Ｓａｓａｙａｍａ剖面和秩父地体Ｔｅｎｊｉｎｍａｒｕ剖面，获得了全岩硫化物的系统硫同位素资料。这两条剖面跨越Ｐ／Ｔ界线，以燧石为主、为连续远洋沉积层序。Ｐ／Ｔ界线以硅质页岩和黑色碳质泥岩组合为特征，黑色碳质泥岩外貌与世界广布的Ｋ／Ｔ界线粘土岩相似。     

滑坡的分形特征

巨型滑坡的滑动块体具有分形特征。其宽度分维数平均为１．２４长度分维娄秋１．４４。我们所获得的有限资料表明分九与基岩地质条件无关。主要根据滑坡块体的大小，巨型滑坡可以分为一级滑块、二级滑块和三级滑块。二级和三级滑块坡体也具有特定的分维数。没扮形特片可以由以下两方面解释：１）自相似性几何形状和２）二级和三级滑块组合产生的特定的分维数。     

Wintertime stratospheric anomalies— Part II: Sudden warmings

The process of stratospheric sudden warmings from development of planetary waves to the sudden cooling after reversal of mean zonal circulation will be studied with the primitive equations of heat and momentum balances. It will be explained that the sudden warmings may occur only in the polar regions of winter stratosphere where zonal mean temperature decreases poleward. The heating rate in the order of major warmings is produced by developed planetary waves in the stratospheric breaking layers. The particular perturbation structure characterized by large amplitude of wave 1 together with minimum of wave 2 discovered by Labitzke (1977) is crucial for initiation of major warmings. The cooling by the same mechanism can be produced in the regions with reversed mean temperature gradient.