Assessment of phytoplankton class abundance using fluorescence excitation-emission matrix by parallel factor analysis and nonnegative least squares

The feasibility of using fluorescence excitation-emission matrix (EEM) along with parallel factor analysis (PARAFAC) and nonnegative least squares (NNLS) method for the differentiation of phytoplankton taxonomic groups was investigated. Forty-one phytoplankton species belonging to 28 genera of five divisions were studied. First, the PARAFAC model was applied to EEMs, and 15 fluorescence components were generated. Second, 15 fluorescence components were found to have a strong discriminating capability based on Bayesian discriminant analysis (BDA). Third, all spectra of the fluorescence component compositions for the 41 phytoplankton species were spectrographically sorted into 61 reference spectra using hierarchical cluster analysis (HCA), and then, the reference spectra were used to establish a database. Finally, the phytoplankton taxonomic groups was differentiated by the reference spectra database using the NNLS method. The five phytoplankton groups were differentiated with the correct discrimination ratios (CDRs) of 100% for single-species samples at the division level. The CDRs for the mixtures were above 91% for the dominant phytoplankton species and above 73% for the subdominant phytoplankton species. Sixteen of the 85 field samples collected from the Changjiang River estuary were analyzed by both HPLC-CHEMTAX and the fluorometric technique developed. The results of both methods reveal that Bacillariophyta was the dominant algal group in these 16 samples and that the subdominant algal groups comprised Dinophyta, Chlorophyta and Cryptophyta. The differentiation results by the fluorometric technique were in good agreement with those from HPLC-CHEMTAX. The results indicate that the fluorometric technique could differentiate algal taxonomic groups accurately at the division level.     

The Gastric Shield and the Undrelying Epithelium of Chlamys Farreri:Morphological,Histological and Histochemical Characterizations

The gastric shield and underlying stomach epithelium of Chlymas ffarreri were examined at the light and ultrastructural levels.The results showed that the gastric shield consisted of two different size lobes joined together by a narrow middle piece,the thicker lobe was shaped like a funnel,but unclosed at the lateral side;the other lobe was irregularly triangular-shaped.The transverse section of the thicker lobe was obviously laminated and gradually decreased in thickness from the peak to the margins of the shield.The underlying epithelium bore numerous about 3μm diameter spherical processes formed by the apical plasmalemma of the epithelial cells becoming blunt pseudopodia.Microvilli and some interspersed cilia were present in the areas among the spherical processes regions where only microvilli existed.Rough endoplasmic reticulum,Golgi apparatus,different-sized electron-dense secretory granules and electron-lucent vacuoles as well las abundant mitochondria were present in the underlying epithelial cells.Fused droplets of the secretion from the underlying epithelial cells formed the gastric shield.     

The suitability of using leaf area index to quantify soil loss under vegetation cover

Soil erosion by water under forest cover is a serious problem in southern China. A comparative study was carried out on the use of leaf area index (LAI) and vegetation fractional coverage (VFC) in quantifying soil loss under vegetation cover. Five types of vegetation with varied LAI and VFC under field conditions were exposed to two rainfall rates (40 mm h⁻¹ and 54 mm h⁻¹) using a portable rainfall simulator. Runoff rate, sediment concentration and soil loss rate were measured at relatively runoff stable state. Significant negative exponential relationship (p < 0.05, R² = 0.83) and linear relationship (p < 0.05, R² = 0.84) were obtained between LAI and sediment concentration, while no significant relationship existed between VFC and sediment concentration. The mechanism by which vegetation canopy prevents soil loss was by reducing rainfall kinetic energy and sediment concentration. LAI could better quantify such a role than VFC. However, neither LAI nor VFC could explain runoff rate or soil loss rate. Caution must be taken when using LAI to quantify the role of certain vegetation in soil and water conservation.     

Synthesis and protein tyrosine phosphatase 1B inhibition activities of two new synthetic bromophenols and their methoxy derivatives

3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (1) is a natural bromophenol isolated from the red algae Rhodomela confervoides that exhibits significant inhibition against protein tyrosine phosphatase 1B (PTP1B). Based on its activity, we synthesized two new synthetic bromophenols and their methoxy derivatives from vanillin using the structure of natural bromophenol 1 as a scaffold. The structures of these bromophenols were elucidated from 1H NMR, 13C NMR, and high resolution electron ionization mass spectrometry as 2,3-dibromo-1-(2p-bromo-6p-(3q,4q-dimethoxybenzyl)- 3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(2),2,3-dibromo-1-(2p-bromo-6p-(2q-bromo-4q,5q-dimethoxy-benzyl)-3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(3),3,4-dibromo-5-(2p-bromo-6p-(2q-bromo-4q,5q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol(4)and 3,4-dibromo-5-(2p-bromo-6p-(3q,4q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol (5).PTP1B inhibition activities of these compounds were evaluated using a colorimetric assay,and compounds 3 and 4 demonstrated interesting activity against PTP1B.     

用GPS位移资料计算应变方法的讨论

目前使用相同或类似的GPS资料求应变,不同研究者给出的结果会有显著差别。为此,对用GPS位移(或速度)资料计算应变(或应变速率)的方法进行了研讨。分析后认为,应变计算可以归结成两大类第一类是利用测点附近资料直接求微分或微分的加权平均值的直接计算法;第二类是用解析曲线拟合观测的位移,然后根据该曲线提供的总的趋势求测点的微分值的位移拟合法,这类方法在拟合中又可以分成整体拟合和分片拟合两种。应变计算应该在球面坐标系下进行,根据研究对象和要求,选择适当的方法。     

Study on deformation law of circular foundation under combined loading

The foundations of some ocean engineering structures are built to withstand not only the vertical gravity load V, but also the horizontal load H induced by sea waves and current. The horizontal load includes the concentrated force load, the moment load M, and the torque load T termed also as combined loading. It is of academic and engineering significance to study the deformation law of submarine seabed due to combined loading. On the basis of the three-dimensional elastic mechanics solution of circular foundation, numerical methods are used to analyze the deformation law of submarine soil under circular foundation with six degrees of freedom. The finite element analysis results give the elastic deformation law of soil in three dimensional spaces, modify the theoretical elasticity solution, and presents nonlinear soil deformation mechanism under the circular foundation with six degrees of freedom.     

Higher species diversity occurs in more fertile habitats without fertilizer disturbance in an alpine natural grassland community

Are there some relationships among species diversity and soil chemical properties of high altitude natural grasslands？ Plant community composition and chemical properties of soil samples were compared to investigate the relationship between soil and species diversity, and the richness in Tibetan alpine grasslands. Results showed that species diversity was significantly positively related to soil organic matter （SOM）, total nitrogen （TN）, available nitrogen （AN）, total phosphorus （TP）, available phosphorus （AP）, and available potassium （AK） in the high alpine grasslands. Margalefs species richness index was also significantly positively related to SOM, TN, AN, and TP. Most soil chemical properties showed significantly positive correlation with species diversity and Margalef＇s richness index. Our results suggested that higher plant species richness index and diversity occurred in more fertile soil habitats in high altitude natural grassland community. In practice, fertilization management for the restoration of degraded grassland should be conducted with reference to the nutrient levels ofnatural grassland without the additional artificial fertilizer and with higher species-diversity and richness index.     

从永夏陈四楼煤矿水文地质特征看岩溶陷落柱并不存在

根据区域和矿区地质资料 ,结合岩溶陷落柱的形成地质条件进行认真的分析研究 ,得出了否定的认识 ,对正确认识永夏矿区的水文地质条件 ,有效地开展矿井防治水工作 ,正确指导生产有重要意义。     

知识经济与科技编辑

以知识经济的特点为背景,论述了知识经济与科技发展的关系,探讨了面对知识经济科技编辑如何处理好基础研究类学术著作、高科技图书与实用普及类图书的出版,以及编辑自身提高等问题。     

Properties and organic activation of ocean clay in the East Pacific

In order to make use of ocean clay reasonably and excavate its potential applications the properties of ocean clay in Chinese investigating area of the East Pacific have been tested with the chemical analysis, XRFS, ICP/MAS, granulometry and SSA, etc. The results show that the clay sediments of this area are rich in clay minerals, fine granularity, bigger SSA, bigger water absorbability and lower ion changing ability etc. To improve the adaptability of ocean clay and macromolecule materials, using the dry milling method with silane, aluminate, stearic acid coupling agents etc the properties of ocean clay are improved. The effect has been evaluated by testing the water absorbing of the modified ocean clay and their dispersing in paraffin liquid. The result is that KH550 is better than any other active reagents, and the best activation technics is that 1.5% (volume) of KH550 quantity, 20min of milling time. The DTA, XRD, SEM and IR analyses and SSA testing show that the organic activation can not change the mineral structure but can densify the structure of clay, and activation mechanism is chemical covered mainly and chemical combined secondly.