Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

Isolation and Characterization of Collagen from Squid(Ommastreoges bartrami) Skin

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations con-firmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts)of PSC were 29.4℃ and 52.8℃, respectively.     

Isolation and Characterization of Collagen from Squid(Ommastrephes bartrami) Skin

Collagen of squid(Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen(ASC) and pepsin-solubilized collagen(PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen,containing α1 and α2 chains. FTIR(fourier transform infrared spectroscopy) investigations confirmed t...     

The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

基于灰关联分析方法卡尔曼滤波器的精度效能评估

针对综导系统卡尔曼滤波器的特点,深入研究了灰关联分析法在滤波器精度效能评估中的应用。给出卡尔曼滤波器进行灰关联分析的方法和步骤,并对某一类型的滤波器进行精度效能评估。仿真结果表明了该方法的可行性和有效性。     

攀枝花“数字城管”中的基础空间信息共享策略研究

本文通过分析“数字城管”实施过程中基础空间信息共享的技术难题,在充分考虑现有的网络条件、软件平台、坐标系统的基础上,提出了基础空间信息共享的技术实现途径,包括适用于通用条件的WMS和WFS技术以及适用于特定软件环境的专用技术,对不同条件的“数字城管”的实施具有一定的参考意义。     

基于NDVI城镇土地利用变化检测探讨

通过归一化植被指数（NDVI）结合影像差值,对经过辐射校正后的1993年和2005年武汉地区TM影像进行了土地利用变化检测,与监督法分类进行比较,得出NDVI更宜于实现变化信息探测和提取。     

西湖凹陷油气成藏的主控因素

西湖凹陷位于东海大陆架边缘,地壳为相对软弱的过渡壳。西湖凹陷中新世末以前属于萎缩型盆地,上新世伴随冲绳海槽的形成才成为扩张性盆地。受构造控制盆地萎缩期沉积有以下3个特点：第一,整体表现为海退;第二,沉积范围逐次减小;第三,泥岩百分比越来越低,盖层条件越来越差。平湖组存在区域盖层,钻遇断块气藏;花港组存在局部盖层,背斜充满度高,断背斜充满度低,断块未成藏;新近系盖层条件更差,断背斜亦未能成藏。由此可见保存条件是西湖凹陷油气成藏的主控因素,保存条件好的领域是西湖凹陷下一步勘探的方向。     

Adaptive output feedback control based on DRFNN for AUV

The tracking control problem of AUV in six degrees-of-freedom (DOF) is addressed in this paper. In general, the velocities of the vehicles are very difficult to be accurately measured, which causes full state feedback scheme to be not feasible. Hence, an adaptive output feedback controller based on dynamic recurrent fuzzy neural network (DRFNN) is proposed, in which the location information is only needed for controller design. The DRFNN is used to online estimate the dynamic uncertain nonlinear mapping. Compared to the conventional neural network, DRFNN can clearly improve the tracking performance of AUV due to its less inputs and stronger memory features. The restricting condition for the estimation of the external disturbances and network's approximation errors, which is often given in the existing literatures, is broken in this paper. The stability analysis is given by Lyapunov theorem. Simulations illustrate the effectiveness of the proposed control scheme.