Spatio-seasonal patterns of fish diversity,Haizhou Bay,China

Spatial-seasonal patterns in fish diversity in Haizhou Bay were studied based on stratified random surveys conducted in 2011.Principal component analysis was conducted to distinguish different diversity components,and the relationships among 11 diversity indices were explored.Generalized additive models were constructed to examine the environmental effects on diversity indices.Eleven diversity indices were grouped into four components:(1) species numbers and richness,(2) heterogeneous indices,(3) evenness,and(4) taxonomic relatedness.The results show that diversity indices among different components are complementary.Spatial patterns show that fish diversity was higher in coastal areas,which was affected by complex bottom topography and spatial variations of water mass and currents.Seasonal trends could be best explained by the seasonal migration of dominant fish species.Fish diversity generally declined with increasing depth except for taxonomic distinctness,which increased with latitude.In addition,bottom temperature had a significant effect on diversity index of richness.These results indicate that substrate complexity and environmental gradients had important influences on fish diversity patterns,and these factors should be considered in fishery resource management and conservation.Furthermore,diversity in two functional groups(demersal/pelagic fishes) was influenced by different environmental factors.Therefore,the distribution of individual species or new indicators in diversity should be applied to examine spatio-seasonal variations in fish diversity.     

DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea

A total of 142 specimens of Ceramiales(Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species,14 genera,and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers,namely,partial large subunit r RNA gene(LSU),universal plastid amplicon(UPA),and partial mitochondrial cytochrome c oxidase subunit I gene(COI). No intraspecific divergence was found in our collection for these markers,except for a 1–3 bp divergence in the COI of Ceramium kondoi,Symphyocladia latiuscula,and Neosiphonia japonica. Because short DNA markers were used,the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in Gen Bank or BOLD(Barcode of Life Data) Systems. Three specimens were presumed as H eterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification,which was the first record in the investigated area,implying that it might be a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers,H eterosiphonia japonica converged with D asya spp. and was distant from the other Heterosiphonia spp.,implying that H. japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species,but the COI had a higher ability to discriminate between species.     

The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

川滇地区断层活动与地震关系研究

选取川滇地区16个跨断层形变观测点,将断层两盘作为不变形的刚体分析断面相对滑动与断层两盘地表点位相对位移的定量关系,计算了各测点的水平扭动量、垂直位移量以及两者之间的比值,并分析了其与地震活动之间的关系。     

汶川地震对湖北地震活动趋势的影响

探讨汶川地震对湖北地震活动的影响,认为龙门山断裂带与襄樊-广济构造带同属于扬子板块西部和北部边缘,是扬子板块逆冲插入青藏板块和秦岭微板块之下形成的。历史强震统计结果表明,四川中西部及周边强震活动对湖北及周边地区中强地震活动影响明显,且对应率较高。汶川地震后3年内,湖北及周边地区具有发生中等强度地震的可能。同时也指出,近几年湖北及周边的地震活动是汶川地震前期东部地区应力场调整的结果。     

Isolation and Characterization of Collagen from Squid(Ommastrephes bartrami) Skin

Collagen of squid(Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen(ASC) and pepsin-solubilized collagen(PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type Ⅰ collagen,containing α1 and α2 chains. FTIR(fourier transform infrared spectroscopy) investigations confirmed t...     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

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Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae)

1　Introduction　Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans .　Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo…     

Natural and artificial connections between atoll islets in the pacific,their process and environmental impact

Three types of natural connection between atoll islets are described. Causeway, as an artificial way of connecting islets, makes a connection to be completed in a few months. In this case shore adjustment becomes more vigorous than that in natural conditions, resulting in lagoon shore erosion. A causeway without short bridges or culverts should be built close to the lagoon shore to reduce lagoon shore erosion. A causeway-crossing channel assemblage is the worst type among all of the connection engineering constructions because the crossing channel traps sediment and further transports it out of the channel, resulting in long period coastal erosion. Reforming Nippon Causeway in Tarawa Atoll, Kiribati by simple means will stop sand loss, reduce shore erosion and greatly upgrade its stability.