Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis>

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.     

Pb distribution and translocation in Jiaozhou Bay

The trends of distribution, translocation and seasonal change of heavy metal Pb were studied based on the surface and bottom water sampling in Jiaozhou Bay in 1979, and compared with those in 1990＇s. The results showed that the source of Pb in the bay was from wastewater and sewage in the east of Jiaozhou Bay from ocean vessels. Pb concentration was higher in spring and lower in summer and autumn, and remained stable through sedimentation in the bottom layer. The overall water quality was good in 1970＇s. Compared with the environmental monitoring data of 1995-1999, Pb pollution had become serious. Therefore, more efforts should be made to protect the bay from Pb pollution.     

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.     

Rossby waves with linear topography in barotropic fluids

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

Method for evaluating the degrees of land use sustainability of mountainous county and its application in Yunnan Province,China

The evaluation of sustainable land use is the key issue in the field of studying the sustainable land utilization. In general analysis, the sustainable land use is evaluated respectively from its ecological sustainability, economic sustainability and social sustainability in China and other countries in recent years. Although this evaluation is an important work, it seems insufficient and hard to comprehensively reflect the whole degree of land use sustainability. Thus, to make up this deficiency, this paper brings forward the evaluation indexes, which make it possible to quantitatively reflect the whole degree of land use sustainability, namely, the concept of ＂degrees of overall land use sustainability＂ （Dos）, and research and measurement development of the method of and calculation in Dos. Taking the evaluation of the degree of land use sustainability in county regions of Yunnan Province as the actual example for analysis, results are basically as follows：
1） The degree of land use sustainability （Dos） is the ration index to organically and systematically integrate the degree of ecological friendliness （DeF）, the degree of economic viability （Dev） and the degree of social acceptability （Dsa）, able to comprehensively reflect the whole sustainability degree of regional land use
2） Based on the value of Dos, the grading system and standard for the sustainability of land use may be established and totally divided into five grades, namely, the high-degree sustainability, middle-degree sustainability, low-degree sustainability, conditional sustainability and non-sustainability. Meanwhile, the standard for distinguishing sustainability grades has also been confirmed so as to determine the nature of sustainability degrees in different grades. This makes the possibility for the combination of nature determination with ration in research result and provides with the scientific guideline and decision-making gist for better implementation of sustainable land use strategy.
3） The pract     

枣庄市山亭区如何进行土地利用现状更新调查

枣庄市山亭区位于枣庄市东北部,泰沂山区西南部,属低山丘陵区,全区大小山头1000多座,地形条件复杂,土地总面积1018．64km^2,山地丘陵占全区总面积的80％以上。山亭区的土地利用现状更新调查工作始于2004年,经过1年多的艰苦调查,查清了全区各类土地利用的现状,绘制了山亭区土地利用现状图,建成了山亭区土地利用数据库。2007年7月,山东省国土资源厅对山亭区的土地利用调查更新工作进行了验收。经验收,山亭区土地利用更新调查技术路线正确、方法先进,各项成果符合土地更新调查技术要求。     

济南北部地热田地热地质特征浅析

济南北部地热田地热资源类型属于层状裂隙岩溶型,热源是北部齐河-广饶断裂的沟通导热,盖层是第四系、新近系、石炭-二叠系、侏罗系.可利用的、具有开发价值的热储层为奥陶系灰岩热储,该热储层顶板埋深在700～2500m左右,热储层厚度由南向北逐渐增大,单井涌水量2000m3/d左右,孔口水温一般在38～57℃,地热田东部地热水矿化度1.4～3.5g/L左右,西部5.1～7.2g/L左右,地热水中富含氟、锶、偏硅酸等微量元素,具腐蚀性,结垢性弱,具较好的开发利用价值.经计算奥陶系热储层单井可采资源量为7.3×105m3/a,合理井间距为5000m.     

文登市加强国土资源文化建设的做法

近年来,文登市国土资源局大力加强国土资源文化建设,不断激发干部活力,提高部门形象力和影响力,极大地促进了国土资源管理工作的开展。1构建和谐进取的精神文化(1)抓理论教育,为精神文化建设奠实基础。坚持把科学发展观的学习作为主题教育的第一讲。每年度下发政治理论学习计划