乳糖诱导重组别藻蓝蛋白基因在大肠杆菌中的表达

以重组别藻蓝蛋白表达工程菌JM109（DE3）／pET28-APC作为研究对象，对于JM109（DE3）菌株在乳糖诱导下表达重组蛋白的规律进行了研究。比较分析了不同生长阶段进行诱导，最佳乳糖浓度、诱导持续时间和诱导温度等参数对重组蛋白表达的影响。实验结果表明，采用JM109（DE3）为宿主菌，经过条件优化，乳糖诱导重组蛋白的表达量可以达到IPTG诱导的水平；较低的诱导培养温度能有效提高可溶性重组蛋白的表达。最后在摇瓶发酵结果的基础上，实现了乳糖诱导工程菌的5L全自动发酵罐培养。研究结果为在大肠杆菌大规模发酵中，运用廉价、无毒的乳糖作为诱导剂提供了一定的借鉴。     

厦门港真刺唇角水蚤的生长率

本文以实验室培养为主,结合自然种群调查,观测厦门港真刺唇角水蚤各发育阶段体长、体重的增长状况,并研究了其生长率与温度、发育期的关系。     

Morphogenetic studies on Uronychia uncinata(Protozoa, Ciliophora) during its asexual division

ＭｏｒｐｈｏｇｅｎｅｔｉｃｓｔｕｄｉｅｓｏｎＵｒｏｎｙｃｈｉａｕｎｃｉｎａｔａ（Ｐｒｏｔｏｚｏａ，Ｃｉｌｉｏｐｈｏｒａ）ｄｕｒｉｎｇｉｔｓａｓｅｘｕａｌｄｉｖｉｓｉｏｎ￥ＳｏｎｇＷｅｉｂｏ（ＲｅｃｅｉｖｅｄＡｕｇｕｓｔ２８，１９９５；ａｃｃｅｐｔｅｄ...     

东海沉积物间隙水中硅酸盐和硫酸盐“扩散-平流-反应”模式的研究

本文提出了东海沉积物间隙水中溶解硅酸盐和硫酸盐的“扩散-平流-反应”模式。研究结果表明,由于间隙水受到硅酸盐溶解、吸附和沉淀不同体系的控制,因而间隙水中的硅酸盐具有三种不同形式的垂直分布,并从模式中得到了上述反应的反应常数,其中E柱硅溶解的一级动力学反应常数为0.00l 42a~(-1)。首次发现了东海沉积物间隙水中硅酸盐指数下降的垂直分布规律,并从数学模式上进行了处理。本文还研完了由于有机质还原sO_4~(2-)而产生的硫酸盐指数下降垂直分布,提出其模式,结果表明,SO_4~(2-)还原最大速率发生在沉积物-水界面附近,每年可达lmmo1/dm~3。     

An Electronic Chart Display and Information System

This article introduces the most advanced form of electronic chart display and information systems (ECDIS) which complies with the performance standards for ECDIS established by the International Maritime Organization (IMO) and International Hydrographic Organization (IHO). It also describes the key features and the important functions of ECDIS, which we developed. This system is different from the productions developed by C-map, Seven-cs, offshore, and others. It is more suitable for China Marine Safety Administration Bureau to manage the navigation marks real-time. Using this system charts were located and accessed rapidly, and displayed smoothly. This article also discusses in ECDIS application system how to dynamically access and desert charts data in a limited memory (limited computer memory and resource). We constructed a tridimensional structure to manage a large amount of charts data. Vertically, according to the scale range, all charts were divided into eight levels, and a data structure called as “Multiple Scale Quad-tree” was constructed. And horizontally, a grid (every unit has a fixed size) was given to a certain scale level. We call this data structure a “Regular Page.” We explore the implementation of such a structure on the worldwide charts management utilizing quad-trees. Then we discuss the future development of ECDIS and its application system in marine agencies in China.     

羊栖菜茎部愈伤组织再生苗的解剖学研究

利用石蜡切片法对羊栖菜纵裂主枝上愈伤组织再生苗的发生过程进行了形态解剖学观察。发现此种成苗方式是在主枝 (茎 )或假根处由外皮层分裂形成愈伤组织 ,再由愈伤组织分化成苗的一种无性繁殖方式。愈伤组织形成再生苗有望成为羊栖菜人工养殖种苗的来源之一。     

平面约束条件在LIDAR点云滤波中的应用

介绍了一种利用平面约束条件对LIDAR点云数据进行滤波的方法,利用每个数据点的邻域点拟合平面,根据平面约束条件和平面点分类方法得到地面点,最后利用地面点内插该区域的DTM.     

The characteristics and sources of natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin

The Central Gas Field is a famous large-sized gas field in the Ordos Basin of China. However, identification of main gas sources of the Ordovician reservoirs in this gas field remains puzzling. On the basis of a lot of geochemical data and geological research on natural gases, the characteristics and sources of natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin were studied. The results indicated that natural gases from Ordovician weathered crust reservoirs in the Central Gas Field in the Ordos Basin have similar chemical and isotopic compositions to highly mature and over-mature dry gases. Both coal-derived gases and oil-type gases coexist in the Central Gas Field in the Ordos Basin. The former was derived mainly from Carboniferous-Permian coal measures and the latter from Lower Paleozoic marine carbonates. It is suggested that coal-derived gases occur in the eastern part of the Central Gas Field while oil-type gases may be produced mainly in the northern, western and southern parts of the Central Gas Field in the Ordos Basin.     

三峡库区高切坡致灾因素及防护对策——以重庆市万州区为例

三峡库区百万移民的搬迁建设导致大量高切坡的形成。未及时治理或治理不当的高切坡,不同程度地发生破坏,影响了人们的正常生活,甚至引发了地质灾害。因此,科学认识及治理高切坡十分必要。重庆市万州区高切坡主要有风化及差异风化、崩塌和土体滑塌等破坏形式。影响破坏的因素主要为工程地质条件、气候及时间效应。针对高切坡不同的破坏形式,以安全、经济及环境和谐的人文理念作为设计指导思想,充分论证了高切坡的防护方案,即：削坡、排水以及格构防护较适合万州区高切坡的防护。这些措施,既能很好地解决高切坡的防护问题,又能兼顾环保、降低造价。研究结果可以作为三峡地区高切坡类地质灾害防护设计的参考。也可为其它流域类似问题借鉴。     

幔源CO_2释出机理、脱气模式及成藏机制研究进展

针对幔源CO2如何从地幔岩浆中脱出并进入沉积地层中形成CO2气藏聚集这一关键问题,总结了国内外研究进展和前缘方向。研究表明,地幔深部的碱性玄武岩浆和碱性岩浆才是深部流体和CO2等挥发份大量赋存、渗滤和释出的场所。浅成侵入岩、次火山岩和火山通道等是CO2释放和聚集的有利位置,岩浆期后和岩浆衰弱期的热液活动阶段是CO2大量释放和聚集的有利时期。幔源CO2进入沉积盆地中具有3种脱气模式,即沿岩石圈断裂直接脱气模式、热流底辟体脱气模式和壳内岩浆房-基底断裂组合脱气模式。CO2的固有物化性质决定其运移相态多样,具有运移和聚集过程同步的特征。只有在满足大量的化学消耗及地层水或原油的溶解和耗散之后才能形成CO2有效聚集。幔源CO2成藏和分布主要受岩浆气源体和气源断裂体系的控制。今后,在超临界CO2及其对油气运移聚集的作用、CO2与深大断裂及火山岩的关系、CO2脱气运移机制、CO与常规烃类油气的耦合差异成藏机制等方面仍需要进一步的研究和探索。