Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

微型近红外光谱仪测量北极积雪覆盖海冰内部太阳辐照度可行性研究

The extremely low temperature, high humidity and limited power supply pose considerable challenges when using spectrometers within the Arctic sea ice. The feasibility of using a miniature low-power near-infrared spectrometer module to measure solar radiation in Arctic sea ice environments was investigated in this study.Temperature and integration time dependences of the spectrometer module were examined over the entire target operating range of –50℃ to 30℃, well below the specified operating range of this spectrometer. Using these observations, a dark output prediction model was developed to represent dark output as a function of temperature and integration time. Temperature-induced biases in the saturation output and linear operating range of the spectrometer were also determined. Temperature and integration time dependences of the signal output were evaluated. Two signal output correction models were developed and compared, to convert the signal output at any temperature within the operating temperature range and integration time to that measured at the reference temperature and integration time. The overall performance of the spectrometer was evaluated by integrating it into a refined fiber optic spectrometry system and measuring solar irradiance distribution in the ice cover with thickness of 1.85 m in the Arctic during the 9th Chinese National Arctic Research Expedition. The general shape of the measured solar irradiance above the snow surface agreed well with that measured by other commercial oceanographic spectroradiometers. The measured optical properties of the sea ice were generally comparable to those of similar ice measured using other instruments. This approach provides a general framework for assessing the feasibility of using spectrometers for applications in cold environments.     

青岛地区海雾分布及大气边界层条件分析

青岛地区海雾多发,观测表明海雾对沿海地区影响范围不尽相同,特别是海雾影响内陆的机理尚缺乏研究。本文利用观测资料及数值模式统计了青岛地区4月-7月海雾分布特征,并对不同影响范围海雾典型个例进行对比分析,结果表明:海雾发生日数自沿海向内陆递减。胶州湾沿岸雾日数比黄海沿岸明显减少,胶州湾东北部的雾日数要少于胶州湾西北部。海雾多发生于高空形势稳定,低层偏南流场的天气条件下。大气边界层内逆温层的的范围大致影响着海雾的分布。只影响沿海的海雾,地面为偏南风,风速在3~8 m/s之间,内陆风力减弱不明显。500 m以下大气边界层内风速切变大。湍流作用使海雾向内陆推进过程中倾斜抬升为低云,地面雾区减弱。能够影响内陆地区的海雾,多出现在地面风力较弱的情况之下,大部分在1~3 m/s之间。500 m以下大气边界层内风速切变小,大气边界层内湍流强度不强,使沿海到内陆的逆温层能够始终维持,沿海海雾在弱南风影响下延伸影响内陆地区。     

青海团宝山花岗岩年代学、地球化学特征及其地质意义

青海省团宝山花岗岩位于南祁连东段,侵位于古元古代化隆群中.为了解其侵位时代、岩体成因和产出构造环境,利用LA-ICP-MS锆石U-Pb定年和地球化学测试方法对团宝山花岗岩岩体样品进行分析.获得其锆石U-Pb年龄为(776±8)Ma;该岩体SiO2、Al2O3和K2O含量较高,而FeOT、MgO和CaO含量较低,K2O/...     

Major contribution to carbon neutrality by China’s geosciences and geological technologies

In the context of global climate change, geosciences provide an important geological solution to achieve the goal of carbon neutrality, China’s geosciences and geological technologies can play an important role in solving the problem of carbon neutrality. This paper discusses the main problems, opportunities, and challenges that can be solved by the participation of geosciences in carbon neutrality, as well as China’s response to them. The main scientific problems involved and the geological work carried out mainly fall into three categories: (1) Carbon emission reduction technology (natural gas hydrate, geothermal, hot dry rock, nuclear energy, hydropower, wind energy, solar energy, hydrogen energy); (2) carbon sequestration technology (carbon capture and storage, underground space utilization); (3) key minerals needed to support carbon neutralization (raw materials for energy transformation, carbon reduction technology). Therefore, geosciences and geological technologies are needed: First, actively participate in the development of green energy such as natural gas, geothermal energy, hydropower, hot dry rock, and key energy minerals, and develop exploration and exploitation technologies such as geothermal energy and natural gas; the second is to do a good job in geological support for new energy site selection, carry out an in-depth study on geotechnical feasibility and mitigation measures, and form the basis of relevant economic decisions to reduce costs and prevent geological disasters; the third is to develop and coordinate relevant departments of geosciences, organize and carry out strategic research on natural resources, carry out theoretical system research on global climate change and other issues under the guidance of earth system science theory, and coordinate frontier scientific information and advanced technological tools of various disciplines. The goal of carbon neutrality provides new opportunities and challenges for geosciences research. In the future, it is necessary to provide theoretical and technical support from various aspects, enhance the ability of climate adaptation, and support the realization of the goal of carbon peaking and carbon neutrality.     

基于2030年可持续发展目标的吉林省城镇化可持续发展评价

结合联合国2030年可持续发展目标（SDGs）构建城镇化可持续发展评价指标体系,从省内、外两个角度研究吉林省城镇化可持续发展状况。结果表明：吉林省城镇化可持续发展水平波动上升,发展趋势良好,大致可分为3个阶段;研究期内各子系统可持续发展水平均呈上升趋势,其中社会子系统上升幅度最大,经济子系统上升幅度最小。城镇化可持续发展水平空间格局呈“核心-边缘”结构,即长春市水平高、其它地区水平低;经济、社会、资源和环境子系统可持续发展水平总体上表现为“中东部高,西部低”。研究期内吉林省城镇化可持续发展水平低于黑龙江省,2015年后高于辽宁省;相较辽黑两省,吉林省社会子系统可持续发展水平较低。     

1983—2017年宁夏盐池县生态治理政策的类型与变化——基于政策工具视角

政策工具是政府实施政策的手段。结合盐池县1983-2017年出台的生态治理政策,运用内容分析法,从政策工具视角,对盐池县生态治理政策的类型与变化进行了分析,并深入探讨其原因。结果表明:强制型和混合型政策工具占主导地位,其中,规制、直接提供、信息与劝诫、补贴与奖励4种工具使用频率最高,自愿型工具的使用还很缺乏。政策工具的运用受到中央政策的显著影响。以后应当开发更多可利用的政策工具,并对不同政策工具进行优化组合。通过本研究可深入了解生态脆弱区地方政府生态政策的政策工具使用情况,为生态脆弱区生态保护的政策工具选择与优化组合提供参考。     

宣郁通经汤合少腹逐瘀汤加减治疗子宫内膜异位症痛经48例总结

目的：观察宣郁通经汤合少腹逐瘀汤加减治疗子宫内膜异位症痛经的临床疗效。方法：将96例子宫内膜异位症痛经患者随机分为2组,每组各48例。治疗组予宣郁通经汤合少腹逐瘀汤加减治疗,对照组予米非司酮治疗,疗程均为4个月。比较2组综合疗效及治疗前后痛经积分的变化情况。结果：总有效率治疗组为93.75%,对照组为75.00%,2组比较,差异有统计学意义（P<0.05）;2组痛经积分治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P<0.01）。结论：宣郁通经汤合少腹逐瘀汤加减治疗子宫内膜异位症痛经较单用米非司酮治疗效果更好。     

基于作战概念的深远海水下作战体系设计与评估

着眼我深远海水下作战面临的现实困难及未来可能遇到的瓶颈问题,设计深远海水下作战概念。以作战概念为驱动,分析深远海水下作战需求,在作战背景下设计行动构想,构建基于作战概念的深远海水下作战体系,评估优化完善后对研究成果进行应用。     

地基干涉雷达IBIS-S桥跨结构振动变形测量与模态分析

地基雷达干涉测量技术能够以较高的时空采样频率获取线性结构目标整体瞬时变形状态。本文将该技术引入大型桥梁桥跨结构的振动变形测量与模态分析中。对动静载试验过程中的武汉二七长江大桥东岸桥跨结构开展连续变形监测,利用频域分解法计算桥跨结构的自振频率、阻尼比和模态振型。计算结果表明,地基干涉雷达干涉测量技术能够快速探测桥跨结构竖弯模态,特别适用于灾后大型线形结构健康状态的应急检测。