Purification and characterization of an alkaline protease from <Emphasis Type="Italic">Micrococcus</Emphasis> sp. isolated from the South China Sea

Protease is wildly used in various fields, such as food, medicine, washing, leather, cosmetics and other industrial fields. In this study, an alkaline protease secreted by Micrococcus NH54PC02 isolated from the South China Sea was purified and characterized. The growth curve and enzyme activity curve indicated that the cell reached a maximum concentration at the 30th hour and the enzyme activity reached the maximum value at the 36th hour. The protease was purified with 3 steps involving ammonium sulfate precipitation, ion-exchange chromatography and hydrophobic chromatography with 8.22-fold increase in specific activity and 23.68% increase in the recovery. The molecular mass of the protease was estimated to be 25 kDa by SDS-PAGE analysis. The optimum temperature and pH for the protease activity were 50°C and pH 10.0, respectively. The protease showed a strong stability in a wide range of pH values ranging from 6.0–11.0, and maintained 90% enzyme activity in strong alkaline environment with pH 11.0. Inhibitor trials indicated that the protease might be serine protease. But it also possessed the characteristic of metalloprotease as it could be strongly inhibited by EDTA and strongly stimulated by Mn²⁺. Evaluation of matrix-assisted laser desorption ionization/time-of-flight MS (MALDI-TOF-TOF/MS) showed that the protease might belong to the peptidase S8 family.     

Antenna design and implementation for the future space Ultra-Long wavelength radio telescope

In radio astronomy, the Ultra-Long Wavelengths (ULW) regime of longer than 10 m (frequencies below 30 MHz), remains the last virtually unexplored window of the celestial electromagnetic spectrum. The strength of the science case for extending radio astronomy into the ULW window is growing. However, the opaqueness of the Earth’s ionosphere makes ULW observations by ground-based facilities practically impossible. Furthermore, the ULW spectrum is full of anthropogenic radio frequency interference (RFI). The only radical solution for both problems is in placing an ULW astronomy facility in space. We present a concept of a key element of a space-borne ULW array facility, an antenna that addresses radio astronomical specifications. A tripole–type antenna and amplifier are analysed as a solution for ULW implementation. A receiver system with a low power dissipation is discussed as well. The active antenna is optimized to operate at the noise level defined by the celestial emission in the frequency band 1 ? 30 MHz. Field experiments with a prototype tripole antenna enabled estimates of the system noise temperature. They indicated that the proposed concept meets the requirements of a space-borne ULW array facility.     

Study on scattering wave force of horizontal and vertical plate type breakwaters

The interaction between wave and horizontal and vertical plates is investigated by the boundary element method,and the relations of wave exciting force with plate thickness,submergence and length are obtained.It is found that:1) The efficient wave exciting force exists while plate submergence is less than 0.5 m,and the plate is very thin with order O(0.005 m).2) The maximum heave wave exciting force exists,and it is the main factor for surface and submerged horizontal plate while the roll force can be ignored.3) The maximum sway wave exciting force exists,it is the main factor for surface or submerged vertical plate,and the roll force is about 20 times of horizontal plate.     

我国瓯江口和珠江口凤鲚洄游模式和生境利用研究

Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths using electron probe microanalysis. Individuals from the Oujiang River had higher and varied Sr:Ca ratios(expressed as(Sr:Ca)×1 000, 3.83–13.0 average) in the otolith core regions, suggesting that they were born in brackish or sea waters, and that a freshwater habitat might not be necessary for egg hatching and larval growth.While, individuals from the Zhujiang River had lower Sr:Ca ratios(0.39–2.51 average) in the core regions,suggesting a freshwater origin. After hatching, anchovies from the Zhujiang River migrate downstream to the river estuary close to brackish water. Our results demonstrated varied habitat use for spawning during stages of early life history between the two populations, and suggested that such variations are promoting diversity of life history strategies of this species.     

A STUDY ON LAND SYSTEM INNOVATIONS IN THE CONSTRUCTION OF SMALL TOWNS IN CHINA

The economic development in China and the rising of the living standard need to speed up the urbanisa-tion.The development of small towns is an important way to Chinese urbanization.Land use plays a very important role in the development of small towns.However there are many problems in the development of small towns,esp.in land use.The paper first discusses the land problems in the development of small towns.Such as much cultivated land lies idle,under-utilization and waste of land,increasing illegal use of land,unstable contractural relationship for land use.The relationship between the development of small towns and land use is also studied.Then the guidelines for the land system innovations of small towns are put forward.Namely the sustainability of social and economic development,the bal-ance between land reservation and land utilization,the provision of service to village ,agriculture and farmer,the manage-ment of land resource and land assets,the parsimonious andlegal use of land.The basic framework of land system innova-tions of small towns is put forward finally.It include the land replacement policy for small towns and the permanent tenan-cy of farmland,the overall plan for land uses and other plans in harmony,the establishment of a flexible system of land supplies,using land with payments ,the transfer of agricultural land and refining the land law related to the construction of small towns.     

Development of a GIS-based catastrophe theory model (modified DRASTIC model) for groundwater vulnerability assessment

This study developed a new paradigm for groundwater vulnerability assessment by modifying the standard DRASTIC index (DI) model based on catastrophe theory. The developed paradigm was called the catastrophe theory-based DI (CDI) model. The proposed model was applied to assess groundwater vulnerability to pollution index (GVPI) in Perak Province, Malaysia. The area vulnerability index was modeled by considering the DRASTIC multiple vulnerability causative factors (VCFs) obtained from different data sources. The weights and ranking of the VCFs were computed by using the inner fuzzy membership mechanism of the CDI model. The estimated vulnerability index values of the CDI model were processed in a geographic information system (GIS) environment to produce a catastrophe theory–DRASTIC groundwater vulnerability to pollution index (CDGVPI) map, which demarcated the area into five vulnerability zones. The produced CDGVPI map was validated by applying the water quality status–vulnerability zone relationship (WVR) approach and the relative operating characteristic (ROC) curve method. The performance of the developed CDI model was compared with that of the standard DI model. The validation results of the WVR approach exhibits 89.29% prediction accuracy for the CDI model compared with 75% for the DI model. Meanwhile, the ROC validation results for the CDI and DI models are 88.8% and 78%, respectively. The GIS-based CDI model demonstrated better performance than the DI model. The GVPI maps produced in this study can be used for precise decision making process in environmental planning and groundwater management.     

Planning and Scheduling of Payloads of AstroSat During Initial and Normal Phase Observations

On 28th September 2015, India launched its first astronomical space observatory AstroSat, successfully. AstroSat carried five astronomy payloads, namely, (i) Cadmium Zinc Telluride Imager (CZTI), (ii) Large Area X-ray Proportional Counter (LAXPC), (iii) Soft X-ray Telescope (SXT), (iv) Ultra Violet Imaging Telescope (UVIT) and (v) Scanning Sky Monitor (SSM) and therefore, has the capability to observe celestial objects in multi-wavelength. Four of the payloads are co-aligned along the positive roll axis of the spacecraft and the remaining one is placed along the positive yaw axis direction. All the payloads are sensitive to bright objects and specifically, require avoiding bright Sun within a safe zone of their bore axes in orbit. Further, there are other operational constraints both from spacecraft side and payloads side which are to be strictly enforced during operations. Even on-orbit spacecraft manoeuvres are constrained to about two of the axes in order to avoid bright Sun within this safe zone and a special constrained manoeuvre is exercised during manoeuvres. The planning and scheduling of the payloads during the Performance Verification (PV) phase was carried out in semi-autonomous/manual mode and a complete automation is exercised for normal phase/Guaranteed Time Observation (GuTO) operations. The process is found to be labour intensive and several operational software tools, encompassing spacecraft sub-systems, on-orbit, domain and environmental constraints, were built-in and interacted with the scheduling tool for appropriate decision-making and science scheduling. The procedural details of the complex scheduling of a multi-wavelength astronomy space observatory and their working in PV phase and in normal/GuTO phases are presented in this paper.     

Oceanic response to Typhoon Nari (2007) in the East China Sea

The oceanic response to a typhoon in the East China Sea (ECS) was examined using thermal and current structures obtained from ocean surface drifters and a bottom-moored current profiler installed on the right side of the typhoon’s track. Typhoon Nari (2007) had strong winds as it passed the central region of the ECS. The thermal structure in the ECS responded to Typhoon Nari (2007) very quickly: the seasonal thermocline abruptly collapsed and the sea surface temperature dropped immediately by about 4°C after the typhoon passed. The strong vertical mixing and surface cooling caused by the typhoon resulted in a change in the thermal structure. Strong near-inertial oscillation occurred immediately after the typhoon passed and lasted for at least 4–5 days, during which a strong vertical current existed in the lower layer. Characteristics of the near-inertial internal oscillation were observed in the middle layer. The clockwise component of the inertial frequency was enhanced in the surface layer and at 63 m depth after the typhoon passed, with these layers almost perfectly out of phase. The vertical shear current was intensified by the interaction of the wind-driven current in the upper layer and the background semi-diurnal tidal current during the arrival of the typhoon, and also by the near-inertial internal oscillation after the typhoon passage. The strong near-inertial internal oscillation persisted without significant interfacial structure after the mixing of the thermocline, which could enhance the vertical mixing over several days.     

Effects of Anthropogenic Disturbance on Sediment Organic Carbon Mineralization Under Different Water Conditions in Coastal Wetland of a Subtropical Estuary

The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P 0.001), and the interaction between human disturbance activities and water conditions was also significant(P 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil aquaculture pond sediment soil near the discharge outlet rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R~2 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.     

社会满意度调查在城市体检与评估中的应用

回顾满意度的概念内涵,在城市研究中的应用进展基础上,总结了北京城市体检及住建部城市体检工作中满意度调查的实施方案和主要分析结论,梳理了社会满意度调查的特征。研究表明,主观满意度数据相比客观数据更为鲜活,能够准确反映不同群体对城市发展的价值判断与意愿,体现居民的关注点,在分析中与人群属性链接可以深挖不同人群的主要诉求,并且根据体检评估对象情况,灵活设置指标和确定优劣等级,易于发现水平差异和问题短板。未来城市体检中应建立常态化城市体检社会满意度调查工作机制,注重与客观数据、市民热线数据等多源数据的融合,主客观相互印证来指导城市规划及管理,从而提升城市建设精细化水平。