西昆仑-喀喇昆仑造山带锂矿成矿特征与成矿规律初探

西昆仑- 喀喇昆仑造山带中生代花岗伟晶岩相当发育,主要分布于麻扎- 康西瓦缝合带以南的喀喇昆仑造山带,构成了西自木吉—塔什库尔干,东到大红柳滩长达600 km的喀喇昆仑稀有金属成矿带。通过多年的研究,本文对西昆仑- 喀喇昆仑造山带37处稀有金属矿床(点)进行了全面系统的梳理,认为喀喇昆仑- 喀喇昆仑造山带表现为“西铍东锂”的格局,稀有金属成矿年龄集中213~206 Ma。将喀喇昆仑造山带稀有金属成矿带划分为木吉- 塔什库尔干稀有金属成矿亚带、赛图拉- 大红柳滩稀有金属成矿亚带,从西向东可划定4个矿化集中区：木吉- 布伦口稀有金属集中区、塔什库尔干- 塔吐鲁沟稀有金属矿化集中区、康西瓦稀有金属矿化集中区、大红柳滩- 白龙山稀有金属矿化集中区。同时,认为西昆仑- 喀喇昆仑造山带西段下一阶段的找矿可放在西合休南锂铍找矿远景区、阿然保泰铍找矿远景区、木吉西锂铍找矿远景区。     

渤、黄海北部海冰年代时空变化特征分析

本文取上世纪后50年至2003年度冬季渤海地区气温作为与渤海冬季冰情有关的气候指标。分析得出,渤海地区冬季气温随着年代呈上升趋势,特别是80年代中期至本世纪初尤为明显。受其影响,渤海冬季的冰情时空分布状况发生了较大变化。其特点是:初冰日较多年平均值推后、终冰日较多年平均值提前,冰期较多年平均值偏短,冰厚较多年平均值偏薄,结冰范围(沿岸固定冰宽度和浮冰外缘线)较多年平均值偏小,甚至有些海区已多年未曾见到海冰了。     

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

临沂市土地整理项目后期管护的做法

临沂市地处沂蒙山区,山区、丘陵、平原各占三分之一。近几年,临沂市政府高度重视土地开发整理工作,自2003年以来,共实施国家级项目17个、省级项目12个、市级项目177个、县级项目1130个,争取国家和省级资金5.3亿元,投入市、县级项目资金6.6亿多元,项目总规模9.8万hm^2,新增耕地2.2万余公顷,新建新修灌溉水渠347.89km,     

平阴县土壤中重金属元素异常成因研究

通过对平阴县周边及其西部农田区采样调查,平阴县周边元素异常具有异常衬度较高,富集系数大等特征,西部农田区异常具有各元素套合关系好,多数异常衬度不高,富集系数小的特点。结果表明,西部农田区异常是以As,Cd为主要成分的异常,元素含量与SiO2,Al2O3,Fe2O3等常量组分间具有显著的相关性,随SiO2含量的增加而降低,随Al2O3,Fe2O3含量的增加而增加,说明土壤常量组分及其所决定的土壤质地是导致异常形成的主要因素;而平阴城区异常是以S,Hg,As,Zn,Mn等元素为主的异常,是典型的人为综合因素（化工烟尘、工厂污水、生活垃圾）产生的异常。     

泥蚶锰超氧化物歧化酶基因的研究

Special primers were designed based on the sequence information of superoxide dismutase genes reported in the Iiterature and were used for RT-PCR and rapid amplification of cDNA ends(RACE) to clone Tegillarca granosaMn-superoxide dismutase gene successfully.The length cDNA of Tegillarca granosaMn-superoxide dismutase gene was of 635bp by including a 3^'untranslated region(UTR)of 230bp and an open reading frame(ORF) of 4.5bp encoding a polypeptide of 135 amino acids. TheTegillarca granosaMn-superoxide dismutase gene shares 66%~73% homology with Mizuhopecteb yessoensis^'s,Danio rerio^'s,Xenopus Laevis^'s,Human^'s,Callinectes sapidus^'sSOD CDS, while the corresponding amino acids shares 68%-78% homology and 80%~89% comparability. These indicate the SOD gene is high evolutional conservation in animals.     

Optimizing support vector machine learning for semi-arid vegetation mapping by using clustering analysis

In remote sensing communities, support vector machine (SVM) learning has recently received increasing attention. SVM learning usually requires large memory and enormous amounts of computation time on large training sets. According to SVM algorithms, the SVM classification decision function is fully determined by support vectors, which compose a subset of the training sets. In this regard, a solution to optimize SVM learning is to efficiently reduce training sets. In this paper, a data reduction method based on agglomerative hierarchical clustering is proposed to obtain smaller training sets for SVM learning. Using a multiple angle remote sensing dataset of a semi-arid region, the effectiveness of the proposed method is evaluated by classification experiments with a series of reduced training sets. The experiments show that there is no loss of SVM accuracy when the original training set is reduced to 34% using the proposed approach. Maximum likelihood classification (MLC) also is applied on the reduced training sets. The results show that MLC can also maintain the classification accuracy. This implies that the most informative data instances can be retained by this approach.     

东营凹陷低渗透油藏分布规律及储层改造效果

东营凹陷2001年之前探明的低渗透油藏主要分布在洼陷带、中央背斜带及北部陡坡带,以沙三段深水浊积扇及沙四段滨浅湖相砂岩滩坝、扇三角洲前缘等沉积体系为主。油藏类型主要是岩性油藏,其次为构造—岩性复合型油藏。低渗透油藏以中深层、常压-超高压、低产、中产为特征,多为特小型、小型油藏,储量丰度为低丰度~特低丰度。低渗透油藏的探井试油过程中,经历了从酸化逐步到压裂的技术发展过程。统计探井的试油效果表明,沙四段碳酸盐含量较高的砂岩低渗透油藏,酸化效果较好;黏土含量较高的沙三段低渗透油藏,压裂效果较好。     

Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.