Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS

Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode. Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode. The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode; the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances. As a result, 18 lipid molecular species were identified for the first time in this microalga, comprising seven phosphatidylcholines (PC), two phosphatidylethanolamines (PE), two phosphatidylinositols (PI), and seven phosphatidylglycerols (PG). Lipid standards of PC, PE, PI, and PG were added to the total lipids as internal standards for semiquantitative analysis, revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg. This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.     

Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65°C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.     

Study on biogenic amines in various dry salted fish consumed in China

This study was carried out to investigate the biogenic amines（BAs）, physicochemical property and microorganisms in dry salted fish, a traditional aquatic food consumed in China. Forty three samples of dry salted fish were gathered from retail and wholesale markets and manufacturers, which had been produced in various regions in China. Cadaverine（CAD） and putrescine（PUT） were quantitatively the most common biogenic amines. About 14% of the samples exceeded the histamine content standards established by the FDA and/or EU. The highest histamine content was found in Silver pomfret（Pampus argenteus）(347.79 mg kg^-1). Five of forty three samples exceeded the acceptable content of TYR(100 mg kg^-1), and 23.26% of dried-salted fish contained high contents of biogenic amines(above 600 mg kg^-1). In addition, species, regions, pickling processes and drying methods made the physicochemical property, microorganisms and biogenic amines in dry salted fish to be different to some extents. The total plate count（TPC） was much higher than that of total halophilic bacteria in all samples. The biogenic amines, physicochemical property and microbiological counts exhibited large variations among samples. Furthermore, no significant correlation between biogenic amines and physicochemical property and TPC was observed. This study indicated that dry salted fish may still present healthy risk for BAs, depending on the processing methods, storage conditions among others.     

Isolation and pharmacological activities of bromophenols from Rhodomela confervoides

Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques, their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl) ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549 cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa. Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215).     

用人工地震波研究三峡地区地面地震动衰减特征

利用长江三峡工程坝区及外围深部构造的人工地震波记录资料,进行了相对速度幅值、速度反应谱和加速度反应谱衰减特征分析,同时利用历史地震资料经转换方法给出的地震动衰减规律进行对比,反映出由人工地震记录地震动参数衰减特征与由历史资料转换确定的地震动衰减特征是有差异的,在不同地质条件的局部地区,这一差异更为明显。这些结果可为缺乏强震记录的地区的地震动估计提供参考。     

任一力学量■的矩阵元Fmn的微扰近似计算

本文以能量和波函数的各级微扰近似的基态展开式为基础,推导出任一力学量的矩阵元F_(mn)的各级微扰近似表示式。并且指出只要构成能量展式的级数收敛很快,满足能够应用微扰的条件,那么对于任一力学量F的微扰计算,只要计算矩阵元F_(mn)的前二级近似就已足够精确。     

LC元件参数的数字测量方法与设计

本文着重说明如何将LC元件的諸参数转换为相应的数字量,以适应数字測量的要求。本文采用的方法与设计,具有电路结构简单,易于实现的特点。     

依据GPS数据研究伽师及帕米尔东北侧1994～1998年地壳水平运动及其与强震群的关系

利用伽师强震群区及帕米尔东北侧地区 1994～ 1998年的GPS监测成果 ,用DDA方法模拟了伽师强震群前后该地区地壳运动和应力应变的变化过程。结果表明 :在此期间伽师地区的应变以南北向挤压为主 ,伽师附近最大主应变变化达到 5× 10 - 8/a ,最大剪应变变化也超过 2 .5×10 - 8/a ,明显高于周边地区。伽师强震群的发生与GPS监测成果反映的该地区地壳运动和应力应变的变化存在明显的对应关系。GPS监测成果反映出在伽师地区有一应力应变变化的集中区域。通过在伽师强震群震中分布块体中强行沿其最大剪应力方向 (NE)增加一新的破裂 ,可使该块体应力应变下降至与周围块体相均衡 ,且能量可能沿柯坪断裂向东北方向传递。新增断裂的运动呈左旋 ,并导致其所在块体呈右旋运动     

参数调整对气象预测的B-P网络模型性能的影响

为了探索ＢＰ网络的参数调整特性,进行了参数α、β的选取对ＢＰ算法的收敛速度和模型的稳定性的影响研究。通过ＢＰ网络用于气象预测建模的参数调整个例分析表明：参数α、β的取值对ＢＰ模型的稳定性无显著影响,但参数值的调整尤其是β值的调整对建模的收敛速度有明显的影响。     

影响流域土壤流失的降水量的PPAR(K)预测模型

介绍了投影寻踪自回归（ＰＰＡＲ（Ｋ））的基本思想及其算法。用ＰＰＡＲ（Ｋ）和均生函数（ＭＧＦ）两种方法对泸州市和北碚市降水量的时间序列进行了建模预测。结果表明ＰＰＡＲ（Ｋ）模型具有较高的稳定性和预测精度。