Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

基于多卫星遥感产品的极端降水监测研究

以中国气象局提供的地面观测站点逐小时降水数据为基准数据,综合评估了目前国际上主流的高分辨率多卫星遥感降水在2015年强台风灿鸿所带来的极端降水事件中的表现。结果表明:1所有的卫星遥感降水产品在此次极端降水事件中对实际降水都存在着低估,其中IMERG系列下经过校正的Final产品IMERG-Final-Calibrated表现得最好;2IMERG-Late-Calibrated在实时产品中表现最好,一定程度上可以满足极端降水事件监测对实时性的要求;3总的来说,在此次极端降水事件中,不管是滞时类产品还是实时类产品,IMERG系列卫星降水的表现均要好于TMPA系列下卫星降水的表现。     

Near-source noise suppression of AMT by compressive sensing and mathematical morphology filtering

In deep mineral exploration, the acquisition of audio magnetotelluric (AMT) data is severely affected by ambient noise near the observation sites; This near-field noise restricts investigation depths. Mathematical morphological filtering (MMF) proved effective in suppressing large-scale strong and variably shaped noise, typically low-frequency noise, but can not deal with pulse noise of AMT data. We combine compressive sensing and MMF. First, we use MMF to suppress the large-scale strong ambient noise; second, we use the improved orthogonal match pursuit (IOMP) algorithm to remove the residual pulse noise. To remove the noise and protect the useful AMT signal, a redundant dictionary that matches with spikes and is insensitive to the useful signal is designed. Synthetic and field data from the Luzong field suggest that the proposed method suppresses the near-source noise and preserves the signal well; thus, better results are obtained that improve the output of either MMF or IOMP.     

内蒙古黄旗海不同粒级湖泊沉积物Rb、Sr组成与环境变化

Based on the geochemical elements Rb and Sr in sediments with three different grain size fractions from profile H3 on the northern lacustrine bottomland 13 m above the Huangqihai Lake surface in 1986,the paper investigates the record of palaeolake stand state, sedimentary environmental evolution,and winter monsoon change.First,these samples are separated into three different grain size fractions,i.e.,total sediments,77-20μm and〈20μm. Second,the chemical elements-Rb and Sr-of the grain size separation were tested and analyzed systematically in this paper.Then the elements compositions of these samples are measured using VP-320 mode fluorescence spectrum instrument,respectively.The magnetic susceptibility of these samples is measured using Kappabridge KLY-3 mode instrument made in Czech AGICO Company.The results showed the elements and the ratios varied regularly with the grain size.But the ratio of Rb/Sr in the sediments〈20μm correlates positively with the magnetic susceptibility of these samples.Therefore,the ratio of Rb/Sr in the fraction〈20 μm from the lake sediments reflected the strengthening of the weathering in the deposition sites.It is a good indicator of the summer monsoon-induced weathering and pedogenesis fluctuations and can be used to reconstruct the conditions of the paleoclimate and paleoenvironment.     

Long-term statistics of extreme tsunami height at Crescent City

Historically, Crescent City is one of the most vulnerable communities impacted by tsunamis along the west coast of the United States, largely attributed to its offshore geography. Trans-ocean tsunamis usually produce large wave runup at Crescent Harbor resulting in catastrophic damages, property loss and human death. How to determine the return values of tsunami height using relatively short-term observation data is of great significance to assess the tsunami hazards and improve engineering design along the coast of Crescent City. In the present study, the extreme tsunami heights observed along the coast of Crescent City from 1938 to 2015 are fitted using six different probabilistic distributions, namely, the Gumbel distribution, the Weibull distribution, the maximum entropy distribution, the lognormal distribution, the generalized extreme value distribution and the generalized Pareto distribution. The maximum likelihood method is applied to estimate the parameters of all above distributions. Both Kolmogorov-Smirnov test and root mean square error method are utilized for goodness-of-fit test and the better fitting distribution is selected. Assuming that the occurrence frequency of tsunami in each year follows the Poisson distribution, the Poisson compound extreme value distribution can be used to fit the annual maximum tsunami amplitude, and then the point and interval estimations of return tsunami heights are calculated for structural design. The results show that the Poisson compound extreme value distribution fits tsunami heights very well and is suitable to determine the return tsunami heights for coastal disaster prevention.     

The trade network of the Belt and Road Initiative and its topological relationship to the global trade network

Unimpeded trade is one of the cooperation priorities in the Belt and Road Initiative proposed by China. On 15 May 2017, the Joint Communique of the Leaders Roundtable of the Belt and Road Forum for International Cooperation reaffirmed the participants’ shared commitment to build an open economy and ensure free and inclusive trade. The Belt and Road Initiative (BRI) is not only China's new action to drive its open and global development, but also a platform for an increasing number of countries to explore free and inclusive trade and promote a universal, rule-based, open, non-discriminatory, and equitable multilateral trade system. It is therefore important to examine the topological relationship between the BRI and global trade networks. More specifically, this article first analyzes the community structure of trade networks using a community detection algorithm, and then estimates the topological relationship between different trade communities. The findings of this article are as follows. First, this research identified three trade communities and two sub-communities in the BRI trade network, in which China is the core, Russia is the sub-core of the biggest trade community, and India, United Arab Emirates, and Saudi Arabia are cores of the second trade community (South Asia-West Asia). Second, it identified five trade communities in the global trade network, centred on China, USA, Russia, India-United Arab Emirates, and Germany- Netherlands-France-Britain and other European developed countries. Third, the topological analysis indicated that in the global trade network, most BRI countries are attracted by the core nodes of the BRI regions, such as the China, Russia and India-United Arab Emirates core nodes, and have strong trade contacts with BRI countries. Most Central-East European countries are mainly attracted by Germany-Netherlands-France-Britain and other developed European countries with a low penetration of BRI trade. Although some Southeast Asian countries are incorporated into the Asia-Australia-South Africa community with China as the core, they still need to strengthen trade linkages with BRI countries.     

Potential implications of differential preservation of testate amoeba shells for paleoenvironmental reconstruction in peatlands

Testate amoebae are now commonly used in paleoenvironmental studies but little is known of their taphonomy. There is some experimental evidence for differential preservation of some testate amoeba shell types over others, but it is unclear what, if any impact this has on palaeoenvironmental reconstruction. To investigate this issue we looked at palaeoecological evidence for the preservation of different shell types. We then investigated the possible impact of selective preservation on quantitative palaeoenvironmental inference. We first used existing palaeoecological data sets to assess the vertical patterns of relative abundance in four testate amoeba shell types: (1) shells made of secreted biosilica plates (idiosomes, e.g. Euglypha), (2) idiosomes with thick organic coating (Assulina), (3) proteinaceous shells (e.g. Hyalosphenia), (4) shells built from recycled organic or mineral particles (xenosomes) (e.g. Difflugia, Centropyxis). In three diagrams a clear pattern of decay was only observed for the idiosome type. In order to assess the implications of differential preservation of testate amoeba taxa for paleoenvironmental reconstruction we then carried out simulations using three existing transfer functions and a wide range of scenarios, downweighting different test categories to represent the impact of selective test decomposition. Simulation results showed that downweighting generally reduced overall model performance. However downweighting a shell type only produced a consistent directional bias in inferred water table depth where that shell type is both dominant and shows a clear preference along the ecological gradient. Applying a scenario derived from previous experimental work did not lead to significant difference in inferred water table. Our results show that differential shell preservation has little impact on paleohydrological reconstruction from Sphagnum-dominated peatlands. By contrast, for the minerotrophic peatlands data-set loss of idiosome tests leads to consistent underestimation of water table depth. However there are few studies from fens and it is possible that idiosome tests are not always dominant, and/or that differential decomposition is less marked than in Sphagnum peatlands. Further work is clearly needed to assess the potential of testate amoebae for paleoecological studies of minerotrophic peatlands.     

Loss of preferred habitat and pollen limitation threatens reproduction in a rare mountain <Emphasis Type="Italic">Paeonia delavayi</Emphasis>

Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China. Recent agricultural land expansion, however, not only causes its dwindling population size, but also poses a severe threat to its long-term persistence. Since our knowledge of the reproductive biology of P. delavayi is very limited, and some management misconceptions have further exacerbated the already worrisome situation, the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P. delavayi, and to help correct some management misconceptions. Specifically, morphological traits of P. delavayi populations growing in two different habitats (i.e., the open area versus forest understory), including basal stem diameter, plant height and flower numbers per plant, were measured and compared with each other. A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation. The results showed that P. delavayi preferred the open habitat over forest understory as it generally grew bigger with thicker stems, and produced a higher number of flowers in the former habitat. Therefore, the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land; also, P. delavayi is pollen limited, as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments (e.g., non-supplemental self pollen, non-supplemental non-self pollen and supplemental self pollen), which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants. We suggest that human intervention might be necessary to guarantee the long-term persistence of P. delavayi as harsh alpine environment, intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.     

Multi-scale coupling analysis of urbanization and ecosystem services supply-demand budget in the Beijing-Tianjin-Hebei region,China

Journal of Geographical Sciences - Rapid economic development and human activities have severely affected ecosystem function. Analysis of the spatial distribution of areas of rapid urbanization is...     

Dynamic Prediction Model of Overburden Settlement in Deep Mining Areas based on Space–Time Relationship

Natural Resources Research - Depletion of shallow mineral resources caused by deep mining has become an inevitable trend, and deep mining can increase safety accidents and geological hazards....