Influence of dietary probiotic Bacillus TC22 and Prebiotic fructooligosaccharide on growth, immune responses and disease resistance against Vibrio splendidus infection in sea cucumber Apostichopus japonicus

The effects of probiotic Bacillus TC22 (isolated from intestine of infected sea cucumber) and prebiotic fructooligosaccharide (FOS) on growth, immunity and disease resistance in sea cucumber Apostichopus japonicus were studied. Six experimental diets were formulated with combinations of three levels of TC22 (0, 107 and 109 CFU g-1 diet) and two levels of FOS (0 and 0.5%) in a 3 × 2 factorial experiment. At the end of the 8-week feeding trial,animals were challenged by injecting Vibrio splendidus. The results revealed that the specific growth rates (SGR) of sea cucumbers were not affected by TC22 and FOS, or the interaction between TC22 and FOS (P > 0.05). However,there were significant interactions between TC22 and FOS for immune response and disease resistance in sea cucumbers (P < 0.05). When sea cucumbers were fed with TC22 at 109 CFU g?1 feed and 0.5% FOS alone or in combination, the phagocytosis, respiratory burst and phenoloxidase activity of sea cucumber coelomocytes were significantly enhanced;the disease resistance against V. splendidus infection was also increased significantly (P < 0.05).However, dietary combination of TC22 at 109 CFU g-1 diet and 0.5% FOS did not yield significantly higher efficiency than either TC22 at 109 CFU g-1 diet or 0.5% FOS used alone (P > 0.05). Therefore, further studies should examine the effects of combinations of other levels of FOS(> 0.5% or < 0.5%) and TC22 on the immunity and disease resistance of sea cucumbers.     

淀山湖主要放流鱼种鲢和鳙生长的研究

本文根据2006-2008年淀山湖鲢鳙标记回捕数据以及刺网调查数据,研究了淀山湖鲢鳙的生长,根据鱼类体长推算公式分别推算出鲢鳙的生长指标;并计算出淀山湖鲢鳙的体长体重关系和肥满度.体长和体重关系分别为鲢:W=6×10-5 L2.8371,(n=216,R2=0.8886);鳙:W=5×10-5L2.5609,(n=55...     

中西太平洋金枪鱼延绳钓渔获组成及其多样性分析

根据2009年6～11月金枪鱼延绳钓观察员海上采集的数据,对中西太平洋S2°05′～S7°51′,W159°48′～W168°10′海域的延绳钓渔获物组成进行了分析.126个站点(钩次)的种类组成分析表明,该作业区域的渔获物有鱼类35种,隶属于9目22科30属;鲸类2种,隶属于1目2科2属;龟鳖类1种.反映种类组成多样...     

Application of stable isotope techniques in studies of carbon and nitrogen biogeochemical cycles of ecosystem

Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.     

Phosphorus biological cycle in the different Suaeda salsa marshes of the Yellow River estuary, China

Much uncertainty exists in the phosphorus (P) cycle in the marshes of the intertidal zone. This study explored the P cycling in the two Suaeda salsa marshes [middle S. salsa marsh (MSM) and low S. salsa marsh (LSM)] of the Yellow River estuary during April 2008 to November 2009. Results showed seasonal fluctuations and vertical distributions of P in different S. salsa marsh soils, and variations in P content in different parts of plants due to water and salinity status. The N/P ratios of the different S. salsa were 9.87 ± 1.23 and 15.73 ± 1.77, respectively, indicating that plant growth in MSM was limited by N, while that in LSM was limited by both N and P. The S. salsa litter in MSM released P to the environment throughout the year, while that in LSM immobilized P from the environment at all times. The P absorption coefficients of S. salsa in MSM and LSM were very low (0.0010 and 0.0001, respectively), while the biological cycle coefficients were high (0.739 and 0.812, respectively). The P turnovers among compartments of MSM and LSM showed that the uptake amounts of roots were 0.4275 and 0.0469 g m^?2 year^?1 and the values of aboveground parts were 1.1702 and 0.1833 g m^?2 year^?1, the re-translocation quantities from aboveground parts to roots were 0.8544 and 0.1452 g m^?2 year^?1, the translocation amounts from roots to soil were 0.0137 and 0.0012 g m^?2 year^?1, the translocation quantities from aboveground living bodies to litter were 0.3157 and 0.0381 g m^?2 year^?1, and the annual return quantities from litter to soil were less than 0.0626 and ?0.0728 g m^?2 year^?1 (minus represented immobilization), respectively. P was an important limiting factor in S. salsa marshes, especially in LSM. S. salsa was seemingly well adapted to the low-nutrient condition and the vulnerable habitat, and the nutrient enrichment due to the import of N and P from the Yellow River estuary would be a potential threat to the S. salsa marshes.     

四川盆地南部下三叠统飞仙关组一段台内鲕滩的主控因素

四川盆地飞仙关组台缘鲕滩气田的发现,激励了人们重新认识台内鲕滩的发育分布规律及其勘探前景。根据野外露头及钻孔资料的分析,认为四川盆地南部飞仙关组一段的台内鲕滩具有发育向上变浅序列、单滩体厚度一般小于3m、横向连续性较差的特征。着重分析了沉积期古地貌、古水深、海水动荡程度以及古海平面相对升降变化对台内鲕滩的控制作用,并把滩体的发育阶段分为雏滩期(低速建造期)、滩核期(高建造期)及衰亡期3个阶段。结果表明,在缓慢等效的海退过程中,处于高能沉积界面附近的水下古高地有利于台内鲕滩的形成,继承性的泸州古隆起核部是区内有利的滩体发育区,形成的台内滩体具有一定的厚度规模和较大的分布面积。这一认识可为该区飞仙关组台内鲕滩的下一步勘探提供必要的地质支持。     

新疆阿舍勒-冲乎尔地区泥盆纪相动力学研究

阿舍勒－冲乎尔火山沉积盆地是一个以火山岩并伴有陆源和内源沉积物的堆积场所，即该区泥盆纪地层是以事件作用的产物为其主要成分特点，显然，对其古地理的恢复，相的划分采用传统的相序分析方法是不合适的。作者认为要阐明该区泥盆纪的古地理面貌，必须在区域地层研究基础上，综合分析各种标志和沉积物的特点，寻求它们的物理成因和地质成因解。     

REE Geochemistry of the Lead—Zinc Ores from the Jinding Lead—Zinc Deposit,Lanping,Yunnan

Nineteen volcanic and magmatic rock samples were collectecd from the Jinding leadzinc deposit and its surrounding areas in Yunnan.The ICP and AES analyses,feferred to the previous results,show that the metal minerals and altered rocks in the Jinding lead-zinc deposit display a decreasing trend of ∑REE from the early to late stages of mineralization,and similarities in REE distribution patterns,indicating that the ore fluids are characterized by high LREE enrichment,markedly negative δCe anomaly and slight δEu anomaly.These REE distribution paterns exhibit striking similarities to those of the Pliocene trachyte in the study area,both of which are similar in ∑REE,LREE/HREE ratio,δEu and δCe.The ore fluids besides the basin fluids in the deposit are also closely related to those associated with Pliocene trachyte magmas.     

兰坪中新生代沉积盆地演化

兰坪中新生代沉积盆地形成和演化与金沙江洋的俯消减及洋陆转换过程密切相关,记录了其盆－山转换过程,早二叠世晚期－晚二叠世时期,由于金沙江洋的俯冲消减,形成了金沙江弧－盆系的空间配置,兰坪地区成为弧后盆地,早中三叠世,金沙江弧－盆系及东西两侧的昌都－兰坪陆块和中咱－中甸陆块的构造沉积式样发生大的转米,开始了兰坪中新生代盆－山转换历史,由于弧陆碰撞作用,使得兰坪分国地由弧后盆地转化成弧后前陆舅地,盆地中     

中国煤系高岭岩(土)资源成矿机理与开发利用

中国含煤地层中高岭岩（土）分布广泛，成矿时代多，成因复杂，储量丰富，质地优良，是一种重要的自然资源和非金属矿产，具有极高的开发利用价值，在全世界独具特色。本文研究了中国煤系高岭岩（土）资源概况、研究简史；根据成分、结构、地质产状等特征，提出了中国煤系高岭岩（土）的成因类型；对生物和有机质在其形成中的作用进行了分析和探讨，并结合有机酸溶解矿物模拟实验对其成矿作用做了初步探索；最后对煤系高岭岩的某些关键性加工技术进行了简述。