Characterization of a new lectin involved in the protoplast regeneration of <Emphasis Type="Italic">Bryopsis hypnoides</Emphasis>

A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was ...     

Impacts of soil fauna on litter decomposition at different succession stages of wetland in Sanjiang Plain, China

Litter decomposition is the key process in nutrient recycling and energy flow. The present study examined the impacts of soil fauna on decomposition rates and nutrient fluxes at three succession stages of wetland in the Sanjiang Plain, China using different mesh litterbags. The results show that in each succession stage of wetland, soil fauna can obviously increase litter decomposition rates. The average contribution of whole soil fauna to litter mass loss was 35.35%. The more complex the soil fauna group, the more significant the role of soil fauna. The average loss of three types of litter in the 4mm mesh litterbags was 0.3–4.1 times that in 0.058mm ones. The decomposition function of soil fauna to litter mass changed with the wetland succession. The average contribution of soil fauna to litter loss firstly decreased from 34.96% (Carex lasiocapa) to 32.94% (Carex meyeriana), then increased to 38.16% (Calamagrostics angustifolia). The contributions of soil fauna to litter decomposition rates vary according to the litter substrata, soil fauna communities and seasons. Significant effects were respectively found in August and July on C. angustifolia and C. lasiocapa, while in June and August on C. meyeriana. Total carbon (TC), total nitrogen (TN) and total phosphorus (TP) contents and the C/N and C/P ratios of decaying litter can be influenced by soil fauna. At different wetland succession stages, the effects of soil fauna on nutrient elements also differ greatly, which shows the significant difference of influencing element types and degrees. Soil fauna communities strongly influenced the TC and TP concentrations of C. meyeriana litter, and TP content of C. lasiocapa. Our results indicate that soil fauna have important effects on litter decomposition and this influence will vary with the wetland succession and seasonal variation. Foundation item: Under the auspices of State Key Development Program for Basic Research of China (No. 2009CB421103), Key Program of National Natural Science Foundation of China (No. 40830535/D0101), Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-BR-16, KSCX2-YW-N-46-06)     

Distribution of the nonlinear random ocean wave period

Because of the intrinsic difficulty in determining distributions for wave periods, previous studies on wave period distribution models have not taken nonlinearity into account and have not performed well in terms of describing and statistically analyzing the probability density distribution of ocean waves. In this study, a statistical model of random waves is developed using Stokes wave theory of water wave dynamics. In addition, a new nonlinear probability distribution function for the wave period is presented with the parameters of spectral density width and nonlinear wave steepness, which is more reasonable as a physical mechanism. The magnitude of wave steepness determines the intensity of the nonlinear effect, while the spectral width only changes the energy distribution. The wave steepness is found to be an important parameter in terms of not only dynamics but also statistics. The value of wave steepness reflects the degree that the wave period distribution skews from the Cauchy distribution, and it also describes the variation in the distribution function, which resembles that of the wave surface elevation distribution and wave height distribution. We found that the distribution curves skew leftward and upward as the wave steepness increases. The wave period observations for the SZFII-1 buoy, made off the coast of Weihai (37°27.6′ N, 122°15.1′ E), China, are used to verify the new distribution. The coefficient of the correlation between the new distribution and the buoy data at different spectral widths (ν=0.3−0.5) is within the range of 0.968 6 to 0.991 7. In addition, the Longuet-Higgins (1975) and Sun (1988) distributions and the new distribution presented in this work are compared. The validations and comparisons indicate that the new nonlinear probability density distribution fits the buoy measurements better than the Longuet-Higgins and Sun distributions do. We believe that adoption of the new wave period distribution would improve traditional statistical wave theory.     

The selection of alkaline protease-producing yeasts fi＇om marine environments and evaluation of their bioactive peptide production

A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C, and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.     

桁拖网渔具刚性栅栏对鱼类的分隔效率研究

东海捕虾桁拖网选择性能不佳,对渔业资源的保护和渔业的合理管理带来了负面的影响.刚性栅栏是实现拖网渔具种类选择性捕捞的主要装置,本文根据在吕四渔场开展刚性栅栏海上生产试验,结合SELECT模型分析不同栅条间距(15、20和25mm)的刚性栅栏对短吻舌鳎、小黄鱼和棘头梅童鱼的分隔效率.结果表明,随着栅条间距的增大,栅栏对鱼类的重量分隔率逐渐减小,分隔栅栏对鱼类的50%选择体长(L50S))逐渐增大;对于短吻舌鳎,个体接触分隔栅栏的概率可使用常数来表示,即接触概率与个体尺寸无关,接触概率在0.2-0.4之间,并随着栅条间距的增大而增大(不显著,P>0.05):对于小黄鱼和棘头梅童鱼,所有进入网囊的个体都将接触刚性栅栏;不同鱼类接触栅栏概率的显著差异说明不同鱼类在网囊中的不同行为习性.     

Web服务驱动的地理信息系统研究

Web服务具有松散耦合、高度集成、基于标准规范等优点。将Web服务的这些特性应用到地理信息系统的开发中,提出了Web服务驱动的地理信息系统架构。该架构对现有的Web服务技术进行了改进,引入了服务语义化描述、动态服务组合以及主动服务等概念。最后,对Web服务驱动的地理信息系统进行了测试（以服务发现为例）,验证了系统架构的可行性和合理性。     

抗差卡尔曼滤波在聊城古建筑动态数据处理中的应用

古建筑在日照、风压等因素的影响下,易产生老化变形,一般会采用及时补救的方法,有时并没有分析其根本原因,本文在布设动态监控网,获得监控数据后,用抗差卡尔曼滤波对监控数据进行分析的方法,对变形方向进行预测,预测值与平差值的误差均在限差范围内,且变化方向大致相同,可以将它作为古建筑变形方向的预报,做到防患于未然。     

基于POM的物质输运方程数值格式研究

在三维海洋模式POM基础上建立水质模型,采用中心差分格式、迎风格式以及Smolarkiewicz迎风格式离散物质输运方程.以三维理想水槽中连续源排放的浓度场预测为例,分析3种离散格式求解所得的浓度场.结果表明,3种格式的数值解与解析解的偏差均小于20%.中心差分格式会引起解的震荡,导致物质的反向输移,出现浓度负值.迎风格式能够保证浓度的正值,但该格式带来的数值耗散导致数值解与解析解偏离较大.Smolarkiewicz迎风格式在普通迎风格式基础上引入抗扩散流速,经多次叠代,能有效降低计算中的数值耗散,提高了计算精度.     

无锡市七城区基础控制网整合研究

城市控制网由于分区建设,各区所依据的起算点不一致,使得控制网之间存在缝隙,影响了全市基础地理资料的完整性.结合无锡市七城区存在四个控制网的情形,提出以D级GPs网作框架,采用坐标转换方法实现七城区坐标系统统一的城市基础控制网整合方案,并应用于无锡市七城区控制网整合改造,解决了七城区四个控制网存在的割裂状况,实现了全市七城区坐标系统的统一,为我国其他城市控制网的改造整合提供了有益借鉴.     

基于遥感技术的大安市土地利用现状分析

随着人类活动对环境的影响,人口、资源与环境问题日益突出,由此引起的土地利用变化尤其引起普遍关注。随着遥感平台的多样化和图像分辨率的提高,遥感技术已成为土地利用现状调查研究中不可或缺的手段。遥感技术以其宏观性、实时性、周期性及综合性等特点,为快速、客观、准确地获取土地利用现状信息提供了可能。通过采用2007年大安市SPOT 5遥感影像,对大安市的土地利用类型进行解译,最终得到各类土地的分类数据,以此为基础开展土地利用现状分析。