智能潜器的集成仿真系统

本文介绍了智能潜器集成仿真系统的硬、软件结构．通过这一系统所展示的水下虚拟仿真环境能够在研究和开发智能潜器的控制体系结构、潜器载体的水动力学、信息融合和目标识别等工作中发挥巨大作用     

几种养殖鲍同工酶生化遗传的比较研究

运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。     

A nitrate and silicate budget in the equatorial Pacific Ocean: a coupled physical–biological model study

A coupled physical–biological model was developed to simulate the low-silicate, high-nitrate, and low-chlorophyll (LSHNLC) conditions in the equatorial Pacific Ocean and used to compute a detailed budget in the Wyrtki box (5°N–5°S, 180–90°W) for the major sources and cycling of nitrogen and silicon in the equatorial Pacific. With the incorporation of biogenic silicon dissolution, NH₄ regeneration from organic nitrogen and nitrification of ammonia in the model, we show that silicon recycling in the upper ocean is less efficient than nitrogen. As the major source of nutrients to the equatorial Pacific, the Equatorial Undercurrent provides slightly less Si(OH)₄ than NO₃ to the upwelling zone, which is defined as 2.5°N–2.5°S. As a result, the equatorial upwelling supplies less Si(OH)₄ than NO₃ into the euphotic zone in the Wyrtki box, having a Si/N supply ratio of about 0.85 (2.5 vs. 2.96 mmolm⁻² day⁻¹). More Si(OH)₄ than NO₃ is taken up with a Si/N ratio of 1.17 (2.72 vs. 2.33 mmolm⁻² day⁻¹) within the euphotic zone. The difference between upwelling supply and biological uptake is balanced by nutrient regeneration and horizontal advection. Excluding regeneration, the net silicate and nitrate uptakes are nearly equal (1.76 vs. 1.84 mmolm⁻² day⁻¹). However, biogenic silica export production is slightly higher than organic nitrogen (1.74 vs. 1.59 mmolm⁻² day⁻¹) following a 1.1 Si/N ratio. In the central equatorial Pacific, low silicate concentrations limit diatom growth; therefore non-diatom new production accounts for most of the new production. Higher silicate supply in the east maintains elevated diatom growth rates and new production associated with diatoms dominate upwelling zone. In contrast, the new production associated with small phytoplankton is nearly constant or decreases eastward along the equator. The total new production has a higher rate in the east than in the west, following the pattern of surface silicate. This suggests that silicate regulates the diatom production, total new production, and thereby carbon cycle in this area. The modeled mean primary production is 48.4 mmolCm⁻² day⁻¹, representing the lower end of direct field measurements, while new production is 15.0 mmolCm⁻² day⁻¹, which compares well with previous estimates.     

摄食水平和性别对稀有鮈鲫生长和能量收支的影响

于1995年10月采集本所自繁有ju鲫（约1.03g/ind)，在30℃水温下进行摄食－生长实验，研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响，结果表明，鱼体干物和能量含量不受摄食率和性别的影响，随着摄食率的增加，雌鱼的湿重，干重和能量特定生长率均呈双对数增加，雄鱼湿重特定生长率呈双对数增加，而干物质和能量特定生长率呈指增加，雄鱼生长速度及摄食率低于雌鱼，随着摄食率的增加，食物能损失于粪便中的比例均呈上升趋势，损失于排泄物中的比例变化不大，呈下降趋势，用于代谢的比例呈下降趋势，用于生长的比例呈上升趋势，除饥饿组外，在各个摄食水平，雌鱼食物能用于代谢的比例较雄鱼低，而用于生长的比例较雄鱼高。     

海藻中类胡萝卜素抗超氧自由基活性研究

在植物组织内，类胡萝卜素除具有集光功能外，还具有其他的重要生物学功能，类胡萝卜素作为光保护剂可抵抗光、氧和光敏色素的有害作用，也可作为化学反应物抵抗细胞所产生的化学物质引起的氧化损伤，保护光合系统免受光动力敏化作用的破坏。类胡萝卜素光保护作用的主要机制如下:(1)类胡萝卜素对三重线态敏化剂的淬灭；(2)类胡萝卜素对1O2的淬灭；(3)类胡萝卜素对自由基反应的抑制，它的这种生物学活性在医学领域也具有重要的应用价值。胡萝卜素在生物系统中的保护作用包括失活由电激活的单线态氧(1O2)，失活反应性的化学物质如过氧化物自由基和烷氧基，这些自由基产生于细胞内，并可能会引起有害的氧化作用。类胡萝卜素的保护功能与直接的抗氧化活性或与对细胞内抗氧化水平的调节有关(Krinsky，1990)。对于类胡萝卜素清除脂类过氧化物自由基的能力和作用机理已经进行了详细的研究(Burton et al.，1984; Burton, 1989; Terao，1989; Palozza et al.，1992b; Tsuchihashi et al.，1995)，但对于类胡萝卜素清除超氧阴离子自由基的能力尚未见有详细研究报道。本文以邻苯三酚自氧化体系作为超氧自由基的发生体系，对海藻中分离纯化制备的类胡萝卜素清除该种自由基的能力进行了初步的探讨。     

黄、渤海沿岸海冰测点代表性浅析

通过分析１９８０年以后的海冰资料和卫星云图照片，对黄、渤海沿岸１１个海冰测点的历史变迁进行了评述，浅析了测点的优劣及使用资料时应注意的事项。     

大鹏澳海域水体磷的形态及其分布特征

根据１９９２年１２月（冬季）和１９９３年７月（夏季）对大鹏澳海域２４ｈ定点连续观测和夏季大面调查，统计了该海域各种形态磷的变化范围和平均含量；计算了各种形态磷分别占总磷（ＴＰ）和总溶解磷（ＴＤＰ）的百分比；讨论了各种形态磷之间的相互关系；分析了影响ＴＰ和ＴＤＰ分布的环境因素。     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

海筒螅中3种化学成分的分离鉴定

海筒螅（Tubuaria marina)的94%乙醇提取物，在水中混悬后，用石油醚萃取，石油醚相中的提取物经反复硅胶柱色谱分离和SephadexLH-20凝胶柱色谱分离，得到三个纯净的化合物。经^1HNMR,^13CNMR和DEPT谱鉴定，分别确定为二十一碳一烯，胆甾-5-烯-3β-醇和1-壬基-甘油醚。     

东山湾贝类养殖容量的估算

以初级生产力为基础，应用营养动态模式和沿岸海域能流分析模型，估算东山湾贝类年生产量分别为269331t和295767t，平均282549t。扣除潮间带、潮下带和吊养区浮筏、随着基及延绳上附着的非养殖滤食性附着动物自然现存量35760t，贝类可养殖量为246789t。应用统计分析和逻辑斯谛种群增长模型估计贝类可养面积为11839hm^2，其中缢蛏635hm^2，石蛎1160hm^2,吊蛎1525hm^2，翡翠贻贝633hm^2，菲律宾蛤仔1154hm^2，扇贝698hm^2，江瑶248hm^2，泥蚶601hm^2，巴非蛤4151hm^2，凸壳肌蛤1034hm^2。1998年实际养殖面积和养殖产量分别为10581hm^2和220564t。尚有1258hm^2,26225t的扩大开发潜力。文中还讨论了合理布局和优化养殖结构问题。