Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

山西省土地覆盖时空变化特征及其驱动因子分析

以遥感与GIS为技术支撑,对山西省20世纪80年代到2005年的土地覆盖进行了动态监测,并分析了土地覆盖的变化特征及其驱动因子;监测表明,山西省土地覆盖时空变化特征:(1)变化数量:城镇建设用地和农村聚落面积增加显著,草地有所增加;旱地和水浇地减少明显,森林和河湖滩地略有减少。(2)变化速度:城镇建设用地增速迅猛,沼泽和农村聚落面积增速较快;河湖滩地减少速度最快,其次,是旱地和水浇地减少速度较快。(3)空间转换:以农田和聚落之间的转换为主,另外城镇建设用地的增加包括其扩展融入的农村聚落等;典型草地的增加主要来自旱地;灌丛减少主要是转变成典型草地,其次是转变成灌丛草地;河湖滩地减少主要是变为内陆水体,再是转变成水浇地。驱动力分析表明,区域自然因素相对稳定,对土地覆盖变化的影响较小,人口、经济、农业结构调整、政策和交通等是山西省土地覆盖变化的主要驱动力。山西省土地覆盖变化的总体特征与区域经济发展、产业布局及其工业化、城市化演变的特征基本一致。     

考虑横向效应饱和黏弹性土层中桩的纵向振动

由于饱和土中土体颗粒与孔隙水的相互作用以及桩与土体的不同渗透率,饱和土体中桩基的力学行为与单相土中桩基力学行为有很大的差别。基于饱和多孔介质理论,考虑桩纵向振动时的横向变形及惯性效应,将桩等效为Rayleigh-Love杆,在频率域中研究了饱和黏弹性土层中端承桩纵向振动的动力特性,给出了饱和黏弹性土层和桩纵向振动时动力响应的解析解及桩头复刚度的解析表达式。通过数值计算,给出了桩头动刚度因子和阻尼随激励频率的响应,考察了饱和土物性参数、桩土模量比、桩长径比、泊松比等参数对桩头刚度因子和阻尼的影响。研究表明,对于大直径桩,当外载荷激励频率较大时桩横向效应对桩头刚度因子和阻尼有显著的影响     

智慧地质建设的初步探讨

智慧地质是充分运用新一代信息技术,发现和总结地质演化的时空规律,判断和预测未来发展趋势,集海量专家认知于一体,认识水平可最大程度的符合或逼近地质自然规律,从而使地质成果以智慧的方式服务于人、造福于人。其意义在于以人为本、服务于人,具体内涵表现为实现地质调查监测工作流程的信息化、地质信息资源互联互通的统一化、地质数据分析应用的智能化、地质成果产品服务的精准化。北京市通过不断创新城市地质工作发展理念、搭建由感知到服务的全流程信息化基础,已经基本具备智慧地质的雏形。智慧地质的建设是一个循序渐进的过程,科学谋划、做好顶层设计、夯实地质理论基础、做好信息平台基础搭建是智慧地质当前发展的首要任务。     

Luminescence dating of baked earth and sediments from the Qujialing archaeological site,China

The Qujialing site is a representative Neolithic archaeological site in the middle reaches of the Yangtze River, China. Absence of suitable material for radiocarbon dating in this region makes the timing of the similar sites difficult. Here we applied optically stimulated luminescence (OSL–SAR) and thermoluminescence (TL–SAR) techniques to date the archaeological and natural deposits from the Qujialing site with known age, testing the techniques on samples at archaeological sites in this region. The results showed that the luminescence properties of quartz from sediment and baked earth samples are very similar. The quartz OSL ages obtained for a sediment sample and a baked earth sample from the cultural layer are 5.4 ± 0.3 and 5.1 ± 0.3 ka, respectively. The quartz TL age of the baked earth sample is 5.6 ± 0.5 ka. These dates are consistent with the calibrated radiocarbon ages (4.9 ± 0.1 and 5.1 ± 0.1 ka cal BP (±1σ)) of the two charcoal samples from the cultural layer at a nearby locality, and are also in agreement with the age of Qujialing culture period. The results indicate that the OSL dating techniques can be applied to date similar archaeological sites in the middle reaches of the Yangtze River, China.     

青藏高原东缘都江堰湖相沉积MIS3a阶段的快速气候变化记录

深海氧同位素第3阶段晚期(MIS3a)是青藏高原较为特殊的暖湿时期, 为了解这一时期高原东缘的气候变化细节, 利用AMS14C定年和粒度、总有机碳、正构烷烃等环境代用指标提取了四川都江堰湖相沉积剖面的相应记录.经过校正后的日历年龄显示此剖面沉积于43.6~34.6 kaBP期间, 记录了千年尺度的D-O旋回事件, 其分布时段分别为: DO11(43.6~41.8 kaBP), DO10(41.8~39.2 kaBP), DO9(39.2~37.7 kaBP), DO8(37.7~35.5 kaBP), DO7(35.5 kaBP~未见顶).该剖面总有机碳曲线与南京葫芦洞石笋和格陵兰冰心(GRIP)氧同位素曲线的对比表明, 该区域气候记录对全球高纬地区的冰量变化和亚洲季风演化具有响应; 而中纬度太阳辐射岁差对该区域气候波动的控制作用较强导致了细节上存在差异.      

Copula‐based joint probability function for PGA and CAV: a case study from Taiwan

This study aims to develop a joint probability function of peak ground acceleration (PGA) and cumulative absolute velocity (CAV) for the strong ground motion data from Taiwan. First, a total of 40,385 earthquake time histories are collected from the Taiwan Strong Motion Instrumentation Program. Then, the copula approach is introduced and applied to model the joint probability distribution of PGA and CAV. Finally, the correlation results using the PGA‐CAV empirical data and the normalized residuals are compared. The results indicate that there exists a strong positive correlation between PGA and CAV. For both the PGA and CAV empirical data and the normalized residuals, the multivariate lognormal distribution composed of two lognormal marginal distributions and the Gaussian copula provides adequate characterization of the PGA‐CAV joint distribution observed in Taiwan. This finding demonstrates the validity of the conventional two‐step approach for developing empirical ground motion prediction equations (GMPEs) of multiple ground motion parameters from the copula viewpoint. Copyright © 2016 John Wiley & Sons, Ltd.     

VP宽频带倾斜仪现场自动校准装置设计

为了对VP宽频带倾斜仪进行现场校准,设计了一款基于单片机AT89C51的新型现场自动校准装置。经验证该装置在+8V激励电压情况下与高精度激光干涉仪结合使用,其校准相对误差为0.36%,满足小于1%的校准相对误差要求。     

甘肃祁连山主动源重复探测项目建设及震源重复性分析

甘肃祁连山主动源重复探测激发源位于甘肃省张掖市祁连山西流水水库,2015年7月9日各系统开始正式运行,11月10日完成了为期40天的连续激发实验。观测资料处理结果表明,气枪震源具有良好的一致性和可重复性,整个观测系统能比较清晰地记录到震源激发的信号。祁连山主动源项目建设取得的结果可为主动源探测工作提供宝贵经验和科学借鉴,不断取得的观测数据为跟踪了解祁连山地区的深部结构时空变化提供了可能。     

Seismic dynamic monitoring in CO<Subscript>2</Subscript> flooding based on characterization of frequency-dependent velocity factor

The phase velocity of seismic waves varies with the propagation frequency, and thus frequency-dependent phenomena appear when CO₂ gas is injected into a reservoir. By dynamically considering these phenomena with reservoir conditions it is thus feasible to extract the frequency-dependent velocity factor with the aim of monitoring changes in the reservoir both before and after CO₂ injection. In the paper, we derive a quantitative expression for the frequency-dependent factor based on the Robinson seismic convolution model. In addition, an inversion equation with a frequency-dependent velocity factor is constructed, and a procedure is implemented using the following four processing steps: decomposition of the spectrum by generalized S transform, wavelet extraction of cross-well seismic traces, spectrum equalization processing, and an extraction method for frequency-dependent velocity factor based on the damped least-square algorithm. An attenuation layered model is then established based on changes in the Q value of the viscoelastic medium, and spectra of migration profiles from forward modeling are obtained and analyzed. Frequency-dependent factors are extracted and compared, and the effectiveness of the method is then verified using a synthetic data. The frequency-dependent velocity factor is finally applied to target processing and oil displacement monitoring based on real seismic data obtained before and after CO₂ injection in the G89 well block within Shengli oilfield. Profiles and slices of the frequency-dependent factor determine its ability to indicate differences in CO₂ flooding, and the predicting results are highly consistent with those of practical investigations within the well block.