银河系旋臂结构和运动学的VLBI高精度探测

直接测量恒星形成区的距离;从而确定银河系的旋臂结构以及银河系运动学的工作正在逐步展开.通过对恒星形成区脉泽的多历元VLBI观测,利用类星体和脉泽相位参考技术,精确地测定脉泽的位置、自行和视差.精确的距离能够确定旋臂的位置,从而建立银河系旋臂结构的新模型;运用绝对自行确定该处天体的三维运动,由此精确测定银河系的旋转曲线,...     

现代测绘与数字地球──迎接21世纪的挑战

本文简要介绍了现代测绘性质、作用、地位及本学科的发展方向;较为详细叙述了现代测绘的主要技术的发展状况及 ３Ｓ集成的广阔应用前景;简述了数字地球的概念及其主要支撑技术、与现代测绘关系,及现代测绘的主要任务;在迎接２１世纪的挑战中,提出一些建议,供研究参考。     

Denitrification potential of marsh soils in two natural saline-alkaline wetlands

Little information is available on denitrification potential of marsh soils in natural saline-alkaline wetlands. The denitrification potentials of an open wetland in the floodplain(Erbaifangzi wetland) and a closed wetland(Fulaowenpao wetland) in backwater areas in Jilin Province of Northeast China were monitored by an anaerobic incubation at 30℃ for 25 days. Our results showed that the relative denitrification index(RDI) increased gradually with incubation time, and showed a rapid increase in the first 5 days of incubation. The RDI values declined quickly from surface soils to subsurface soils and then kept a small change in deeper soils along soil profiles over the incubation time. Denitrification proceeded much faster in the top 20 cm soils of open wetland than in the closed wetland, whereas no significant differences in RDI values were observed in deeper soils between both wetlands. The RDIs were significantly negatively correlated with bulk density and sand content, while a significantly positive correlation with clay content, soil organic matter, total nitrogen and phosphorous. The maximum net NO–3-N loss through denitrification in 1 m depth were higher in the open wetland than the closed wetland with higher soil pH values. Future research should be focused on understanding the influencing mechanisms of soil alkalinity.     

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

Impacts of internal waves on chlorophyll a distribution in the northern portion of the South China Sea

Internal waves can bring nutrients to the upper level of water bodies and facilitate phytoplankton photosynthesis. Internal waves occur frequently in the northern portion of the South China Sea and inflict an important effect on chlorophyll a distribution. In this study, in-situ observation and satellite remote sensing data were used to study the effects of internal waves on chlorophyll a distribution. Based on the in-situ observations, lower chlorophyll a concentrations were present in the middle and bottom level in areas in which internal waves occur frequently, while the surface chlorophyll a distribution increased irregularly, and a small area with relatively higher chlorophyll a concentrations was observed in the area around the Dongsha Island. Satellite remote sensing showed that the chlorophyll a concentration increased in the area near Dongsha Island, where internal waves frequently occurred. The results of the increased chlorophyll a concentration in the surface water near Dongsha Island in the northern portion of the South China Sea indicated that internal waves could uplift phytoplankton and facilitate phytoplankton growth.     

Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus

The Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. Bacteria flagellins play an important role during infection and induction of the host immune response. Thus, flagellin proteins are an ideal target for vaccines. We amplified the complete flagellin subunit gene (flaA) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 62.78 kDa. We purified and characterized the protein using Ni-NTA affinity chromatography and Anti-His antibody Western blotting, respectively. Our results provide a basis for further studies into the utility of the FlaA protein as a vaccine candidate against infection by Vibrio parahaemolyticus. In addition, the purified FlaA protein can be used for further functional and structural studies.     

高密市南部贫水区地下水勘查探讨

高密市南部低缓丘陵区是山东省典型的资源型缺水地区,通过遥感解译、水文地质调查、物探、钻探等手段,在小于家庄村南成功施工1眼深100 m水井,并通过多孔非稳定流抽水试验求取了部分水文地质参数,确定了可开采量。此次工作对下一步该区农业抗旱、居民生活用水打井都具有一定的示范意义。     

电磁波透视法在大龙洞水库查漏中的应用

文章介绍了跨孔电磁波透视法探测岩溶水库渗漏位置的基本原理和方法技术，并阐述了该法在国内较典型的且渗漏特别严重的岩溶水库──广西上林大龙洞水库查漏堵漏工程中的应用，效果非常显著。     

Stock discrimination of spottedtail goby (Synechogobius ommaturus) in the Yellow Sea by analysis of otolith shape

Otolith shape is species specific and is an ideal marker of fish population affiliation. In this study, otolith shape of spottedtail goby Synechogobius ommaturus is used to identify stocks in different spawning locations in the Yellow Sea. The main objectives of this study are to explore the potential existence of local stocks of spottedtail goby in the Yellow Sea by analysis of otolith shape, and to investigate ambient impacts on otolith shape. Spottedtail goby was sampled in five locations in the Yellow Sea in 2007 and 2008. Otoliths are described using variables correlated to size (otolith area, perimeter, length, width, and weight) and shape (rectangularity, circularity, and 20 Fourier harmonics). Only standardized otolith variables are used so that the effect of otolith size on the shape variables could be eliminated. There is no significant difference among variables of sex, year, and side (left and right). However, the otolith shapes of the spring stocks and the autumn stocks differ significantly. Otolith shape differences are greater among locations than between years. Correct classification rate of spottedtail goby with the otolith shape at different sampling locations range from 29.7%–77.4%.     

Synthetic information prediction system for crisis mine based on GIS

Reserves of some kinds of the crisis mines will be lack now or from now on, because of lacking seriously reserves of mineral resources and the crisis of exploring bases in support. So that it is urgent to predict, appraise, development and utilize the replaceable resources of the crisis mines. The mineral resources prediction software system of synthetic information is intelligent GIS which is used to quantitative prediction of large-scale synthetic information mineral target. It takes the geological body and the mineral resource body as a unit. And it analyzes the ore deposit genesis and metallotect, knows the spatial distribution laws of the ore deposit and ore body, and establish the prospecting model based on the concept of establishing the three-dimensional space of a mine. This paper will primarily discuss some important problems as follows: the secondary development of various kinds of data(including geology, geophysical prospecting, geochemical prospecting and remote sensing, etc); process synthetically and establish the synthetic information interpretative map base; correspond prospecting model with synthetic information of ore deposit; divided into statistical units of metallogenic information synthetic anomalies based on the synthetic information anomalies of ore control, then research the metallogenic information variable of unit synthetically and make quantitative prediction according to choose the quantitative prediction math model which is suitable to the demands of large-scale precision; at last, finish the target area optimization of ore deposit (body).