Sea cucumber Apostichopus.japonicus is an important marine economic species in Asian countries due to its profound nutritional and medicinal value.So far,with the rapid development of intensified artificial aquaculture of sea cucumbers,the use of antibiotics is still an inexpensive and dispensable way to treat pathogenic infections,especially during the nursery phase.However,there is little information on the effects of antibiotics on the intestinal microbiota of sea cucumber.Therefore an Illumina based sequencing method was used to examine the intestinal bacterial composition of juvenile A.japonicas following diets with three typical antibiotics(tetracycline,erythromycin.,and norfloxacin)under 15,30,and 45 d.The findings reveal that different antibiotics have distinct effects on the growth performance of juvenile sea cucumbers.However,the richness and diversity of microbiota were barely affected by antibiotics but the community composition alterations indicated that the three antibiotics exhibited their respective patterns of reshaping the intestinal bacteria of juvenile sea cucumbers.In common,the abundance of some sensitive genera with helpful functions,such as Thalassotalea,Shewanella,Sulfitobacter,and Halomonas decreased significantly with exposure to antibiotics and the abundance of multiple potential pathogenic-and suspected antibiotic-resistant microorganisms like Arcobacter,Leucothrix,and Clostridium_sensu_stricto_1 was found increased significantly in the antibiotic groups.These results suggest that low doses of antibiotics could affect the composition of the intestinal microbiota of sea cucumbers and might increase the risk of infection of the hosts.This study could help us to explore how antibacterial compounds modify the gut microbiota of sea cucumbers and provide theoretical guidance in hatchery management by scientific antibiotic use in sea cucumber mariculture. 相似文献
Journal of Oceanology and Limnology - A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with... 相似文献
The knowledge of prey small fish stock, distribution and abundance is necessary to guide stocking of piscivorous fish for the biomanipulation in domestic tap water lakes. This study describes the current status of small fish community in Lake Kuilei (China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into five major habitats: (1) macrophyte covered shallow habitat of water depth < 2.00 m, (2) uncovered or less-covered shallow habitat (2.00 m–3.50 m), (3) uncovered medium shallow habitat (3.50 m–5.00 m), (4) uncovered medium deep habitat (5.00 m–6.50 m) and (5) uncovered deep habitat (6.50 m–8.50 m). The abundance and composition of small fish were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance (accounted for 88.96% of the total) and occurrence (more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus, Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small fish species. The results of correlation analysis identified that species richness ( Sr ), Shannon-Wiener diversity index ( H′ ) and Margalef′s richness index ( D ) were significantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis (RDA) revealed that the spatial distributions of most small fishes were negatively associated with water depth. The details of these findings are beneficial to understanding the adaptation of the small fishes in degraded environments, and to developing suitable biomanipulation strategies for the management of fish resources and water quality in the lakes along the lower reach of the Changjiang (Yangtze) River basin.
The population distribution grid at fine scales better reflects the distribution of residents and plays an important role in investigating urban systems. The recent years have witnessed a growing trend of applying the nighttime light data to the estimation of population at micro levels. However, using the nighttime light data alone to estimate population may cause the overestimation problem due to excessively high light radiance in specific types of areas such as commercial zones and transportation hubs. In dealing with this issue, this study used taxi trajectory data that delineate people’s movements, and explored the utility of integrating the nighttime light and taxi trajectory data in the estimation of population in Shanghai at the spatial resolution of 500 m. First, the initial population distribution grid was generated based on the NPP-VIIRS nighttime light data. Then, a calibration grid was created with taxi trajectory data, whereby the initial population grid was optimized. The accuracy of the resultant population grid was assessed by comparing it with the refined survey data. The result indicates that the final population distribution grid performed better than the initial population grid, which reflects the effectiveness of the proposed calibration process. 相似文献
An in-depth analysis of the urban road network structure plays an essential role in understanding the distribution of urban functional area. To concentrate topologically densely connected road segments, communities of urban roads provide a new perspective to study the structure of the network. In this study, based on OpenStreetMap (OSM) roads and points-of-interest (POI) data, we employ the Infomap community detection algorithm to identify the hierarchical community in city roads and explore the shaping role roads play in urban space and their relation with the distribution of urban functional areas. The results demonstrate that the distribution of communities at different levels in Guangzhou, China reflects the urban spatial relation between the suburbs and urban centers and within urban centers. Moreover, the study explored the functional area characteristics at the community scale and identified the distribution of various functional areas. Owing to the structure information contained in the identification process, the detected community can be used as a basic unit in other urban studies. In general, with the community-based network, this study proposes a novel method of combining city roads with urban space and functional zones, providing necessary data support and academic guidance for government and urban planners. 相似文献
Natural Resources Research - An accurate forecasting model for the price volatility of minerals plays a vital role in future investments and decisions for mining projects and related companies. In... 相似文献
We conducted acoustical surveys with a horizontal beam transducer to detect fish and with a vertical beam transducer to detect depth and macrophytes in two typical shallow lakes along the middle and lower reaches of the Changjiang(Yangtze) River in November 2013. Both lakes are subject to active fish management with annual stocking and removal of large fish. The purpose of the study was to compare hydroacoustic horizontal beam estimates with fish landings. The preliminary results show that the fish distribution patterns dif fered in the two lakes and were af fected by water depth and macrophyte coverage. The hydroacoustically estimated fish biomass matched the commercial catch very well in Niushan Lake, but it was two times higher in Kuilei Lake. However, acoustic estimates included all fish, whereas the catch included only fish 45 cm(smaller ones were released). We were unable to determine the proper regression between acoustic target strength and fish length for the dominant fish species in the two lakes. 相似文献