Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

渤海湾天津近岸海域水环境空间变异分析

由于海岸带的地理条件和海洋动力特性的复杂性以及人类开发活动对海岸带的影响,近岸海域的生态环境系统在空间分布上有很大的变异性.基于现场实测资料,建立系统的空间变异分析模型,对海岸带生态环境空间变化规律进行分析,可以充分了解不同区域的污染特征和变化趋势.应用所构建的空间变异分析模型对渤海湾天津近岸海域的水环境特性进行分析,结合对应年份的入海流量及其变化,分析了天津渤海湾海岸带污染的主因子和区域水环境的分布特征,结果可以对海洋功能区的划分和水环境综合评价结果进行验证和补充.     

渤海生态动力过程的模型研究 Ⅱ.营养盐以及叶绿素a的季节变化

利用在本系列研究第一部分中所建立的耦合的生物物理模型,模拟了渤海浮游植物生物量和营养盐含量的年度循环特征.模拟结果显示:藻类的春季水华是由经过一冬积累在水体中的营养盐导致,而水华开始的时间在浅水区明显早于深水区,对此深水区水体层化结构的形成可能起着重要作用;另一方面,河载营养盐与悬起的沉积物所释放的营养盐是诱发夏季水华的共同原因.基于模型结果,我们还发现:渤海的浮游植物动力特性就整体而言依然受无机氮限制,但是在莱州湾,磷限制特性表现得非常明显,这主要是由于每年黄河都要携带大量的无机氮进入海水,从而导致莱州湾营养盐的氮磷比已远远超过16.     

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。     

南极冰藻类囊体膜光系统研究进展

南极冰藻是南大洋碳流和能量流的重要组成部分,在该区域的生态系统中发挥着极其重要的作用.低温、低光照和高盐度对类囊体膜光系统的伤害必将影响冰藻对光能的吸收、传递和转换,从而影响冰藻的正常生长繁殖.事实上,冰藻为了能够在这寒冷骤变的环境中生存、繁衍,类囊体膜光系统在生理、代谢和遗传上进行了复杂的适应性改变,冰藻在低光照条件下保持很高的光合效率.因此研究南极冰藻类囊体膜光系统具有重要的理论意义.综述了南极冰藻类囊体膜光系统的研究进展,以期为进一步了解南极冰藻光系统作用机理提供依据.     

Determination of the Critical Value of Deflection for Embedded Cylindrical Structures

An embedded cylinder is a large-diameter cylinder embedded in a soil foundation. The state of failure of such an embedded cylindrical structure shows large deflection instead of slide and overturn of the traditional gravity type of structure placed on a rubble base or foundation base. The critical value of deflection of the embedded cylindrical structure, which is the maximum allowable deflection for stability calculation of the cylinder, is a vital control value. Through investigation on deflection and soil pressures on an embedded cylinder by model experiments, the variations of the angle of rotation θ of a cylinder with effective anti-overturning ratio η and moment MH of thrust are discussed. On the basis of experimental study, the critical value of deflection of the cylindrical structure is proposed in the paper. Meanwhile, the formulas for calculating deflection of cylinders are derived.     

提取分潮调和常数的新方法——正交方法

给出了提取潮汐调和常数的一种新方法--正交方法,并应用1992～1997年的TOPEX/POSEIDON卫星高度计遥感资料,提取中国海M2分潮调和常数.同时,利用最小二乘法来提取中国海M2分潮调和常数,两种方法结果比较渤海、黄海、东海海域M2分潮振幅、迟角的均方差分别是3.3 cm,3.6°;南中国海海域M2分潮振幅、迟角均方差分别是1.1 cm,1.7°,结果表明正交方法是一种可信的具有实用性的方法.     

1993—2001年全球海面高度变化特征

应用TOPEX／POSEIDON（T／P）卫星高度计测高资料，对全球海洋的海面变化特征进行了分析，结果表明，1993年1月－2001年6月期间，全球海平面呈现上升的态势；全球平均海平面高度的平均上升速率约为1．2mm/a；海温的变化是引起海平面变化的重要原因，便其对海平面抬升的贡献不到50％。海平面的变化具有很强的地域特征。海平面变化的空间分布特征受风应力异常特别是纬向风应力异常的空间分布影响较大。     

ASM1中污水水质参数与化学计量系数的估测

简要介绍了活性污泥1号模型中14个污水水质参数与5个化学计量系数的估测方法和典型取值。     

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.