Distribution characteristics of zooplankton biomass in the East China Sea

On the basis of the data of oceanographic survey in the East China Sea in four seasons during 1997-2000 (23°30'~33°00'N, 118°30'-128°E), the variation of total biomass and diet biomass of zooplankton and their spatial-temporal distribution and relationship with the fishing ground of Engraulis japonicus are approached and analyzed. The results show that the average biomass is 65.32 mg/m3 in four seasons, autumn (86.18 mg/m3) being greater than summer (69.18 mg/m3) greater than spring (55.67 mg/m3) greater than winter (50.33 mg/m3). The average value of diet zooplankton biomass is 40.9 mg/m3. The trends of horizontal distribution both in the total biomass and the diet biomass of zooplankton are similar. The high biomass region (250-500 mg/m3) is very limited, only accounting for 1% of the investigation area. Seasonal variation of the biomass is very remarkable in the west and north parts of East China Sea coastal waters ( 29°30'N,125°E). The horizontal distribution of diet zooplankton depends on the     

新型全封闭刀口联合式管口封的设计及应用

重力活塞取样器是海洋地质调查不可缺少的调查设备，而管口封又是重力活塞取样器的重要部件。管口封的好坏不但影响到取样器的贯入深度和取芯丰，还影响到所取岩芯的质量．介绍了一种最近研制成功的新型全封闭刀口联合式管口封。海上实验在特定海区成功的获取了17．11m的长岩芯，取样率达91．3％，创造了我国海洋地质调查用重力活塞取样器获取岩心的最长记录。该设计已通过国家知识产权局批准授予实用新型专利。     

Monitoring the Kuroshio Extension with Dynamically Constrained Synthesis of the Acoustic Tomography, Satellite Altimeter and in situ Data

A finite-difference quasigeostrophic (QG) model of an open ocean region has been employed to produce a dynamically constrained synthesis of acoustic tomography and satellite altimetry data with in situ observations. The assimilation algorithm is based upon the 4D variational data interpolation scheme controlled by the model's initial and boundary conditions. The data sets analyzed include direct and differential travel times measured at the array of five acoustic transceivers deployed by JAMSTEC in the region of the Kuroshio Extension in 1997, Topex/Poseidon altimetry, CTD soundings, and ADCP velocity profiles. The region monitored is located within the area 27.5°–36.5°N, 143°–155°. The results of assimilation show that mesoscale variability can be effectively reconstructed by five transceivers measuring direct and reciprocal travel times supported by relatively sparse in situ measurements. The misfits between model and data lie within the observational error bars for all the data types used in assimilation. We have compared the results of assimilation with the statistical inversion of travel time data and analyzed energy balances of the optimized model solution. Energy exchange between the depth-averaged and shear components of the observed currents reveals a weak decay of the barotropic mode at the rate of 0.2 ± 0.7⋅10⁻⁵ cm²/s³ due to topographic interaction. Mean currents in the region are unstable with an estimate of the available potential energy flux from the mean current to the eddies of 4.7 ± 2.3⋅10⁻⁵ cm²/s³. Kinetic energy transition has the same sign and is estimated as 2.8 ± 2.5⋅10⁻⁵ cm²/s³. Potential enstrophy is transferred to the mesoscale at a rate of 5.5 ± 2.7⋅10⁻¹⁸ s⁻³. These figures provide observational evidence of the properties of free geostrophic turbulence which were predicted by theory and observed in numerical experiments. This revised version was published online in July 2006 with corrections to the Cover Date.     

黄海海域陆相中生界油气远景

黄海海域油气勘探已40余载，尚未实现油气突破，其找油前景引起石油地质学家的极大关注。根据前人的认识和最新研究成果，结合邻区油气地化资料，探讨了黄海海域中生界地层生、储、盖特征，特别是白垩系，它分布广，厚度大，烃源岩达到较好生油岩标准，并且生、储、盖组合理想，中生界是该区下部油气勘探首选目标。     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

仿鱼尾潜器推进系统的水动力分析

以开发适用于小型潜器的仿生操纵与推进系统为研究背景 ,对金枪鱼的月牙形尾鳍进行水动力分析。文中将金枪鱼的尾鳍处理为在做横移和摇摆的耦合运动的同时 ,以某一匀速向前运动的月牙形刚性尾翼。计算中应用了双曲面元和压力库塔条件 ,利用面元法计算分析该三维尾翼的非定常水动力性能。探讨了前进速度、横荡和摇首的幅度、频率及其相位差对推进性能的影响     

GPS测高技术在无验潮水深测量中的应用

应用双频GPS动态后处理高精度定位技术，建立了一套完整的GPS无验潮海洋深度测量作业模式，通过海上试验与传统作业模式作了数值分析比较，结果表明，该作业模式不仅无需验潮，而且能够有效消除传统作业模式中船只动态吃水和涌浪等因素对测量成果的影响，显著提高水深测量成果的精度。     

海水中液-固界面的台阶型动力学曲线 Ⅰ.动力学曲线之台阶的消长和变化规律

对海水中Zn(Ⅱ),Ca(Ⅱ),Cd(Ⅱ)与高岭石、伊利石和蒙脱石等粘土矿物;无定形水合氧化铁、α-FeOOH等铁的水合氧化物;δ-MaO_2,γ-MnOOH,水锰矿等锰的水合氧化物等30个左右实验体系的液-固界面台阶型动力学曲线进行了系统的实验测定和全面的条件研究。在实验测定上提出两种方法、互为校核。对动力学曲线之台阶的消长和变化规律作了系统研究,确定主要影响因素是:金属离子初始浓度、固体交换剂量、体系的pH值和温度,以及搅拌速度等。     

北太平洋宽吻海豚正常生理指标数值的算法

连续5年对青岛海豚表演馆中北太平洋宽吻海豚(Tursiops gille Dall)进行现场实验和行为学研究，获取了大量的生理指标数据，运用不同的分析和计算方法对所得数据进行了筛选、剔除及处理，确立了海豚各生理指标的正常闽值和范围确定的方法，从而为准确了解海豚机体的生理状况、疾病诊断及治疗奠定了基础。     

凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。