In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

“Trends” and variations of global oceanic evaporation data sets from remote sensing

The variability in global oceanic evaporation data sets was examined for the period 1988-2000. These data sets are satellite estimates based on bulk aerodynamic formulations and include the NASA/Goddard Space Flight Center Satellite-based Surface Turbulent Flux version 2 （ GSSTF2）, the Japanese-ocean flux using remote sensing observations （J-OFURO）, and the Hamburg Ocean-Atmosphere Parameters and Fluxes from Satellite version 2 （HOAPS2）. The National Center for Environmental Prediction （NCEP） reanalysis is also included for comparison. An increase in global average surface latent heat flux （SLHF） can be observed in all the data sets. Empirical mode decomposition （EMD） shows long-term increases that started around 1990 for all remote sensing data sets. The effect of Mt. Pinatubo eruption in 1991 is clearly evident in HOAPS2 but is independent of the longterm increase. Linear regression analyses show increases of 9.4%, 13.0%, 7. 3%, and 3.9% for GSSTF2, J-OFURO, HOAPS2 and NCEP, for the periods of the data sets. Empirical orthogonal function （EOF） analyses show that the pattern of the first EOF of all data sets is consistent with a decadal variation associated with the enhancement of the tropical Hadley circulation, which is supported by other satellite observations. The second EOF of all four data sets is an ENSO mode, and the correlations between their time series and an SO1 are 0.74, 0.71,0.59, and 0.61 for GSSTF2, J-OFURO, HOAPS2, and NCEP in that order. When the Hadley modes are removed from the remote sensing data, the residue global increases are reduced to 2.2% , 7. 3%, and 〈 1% for GSSTF2, J-OFURO and HOAPS, respectively. If the ENSO mode is used as a calibration standard for the data sets, the Hadley mode is at least comparable to, if not larger than, the ENSO mode during our study period.     

基于NS2的TCPW性能改进算法研究

TCP Westwood(TCPW)和Vegas都是基于测量的TCP New-Reno增强算法。TCPW针对ACK流直接估算可用带宽,在慢启动和线性递增部分依然采用传统Reno的盲目递增的机制,导致发送端较为频繁的重传。对这几种算法原理进行分析阐述,并提出1种改进方法,更精确的测量估计带宽,通过网络仿真工具NS2进行验证,明显去除TCP全局同步现象,增强网络性能。     

Base cation concentrations in forest litter and topsoil have different responses to climate and tree species along elevational gradients

The forest litter is an essential reservoir of nutrients in forests, supplying a large part of absorbable base cations(BC) to topsoil, and facilitating plant growth within litter-soil system. To characterize elevational patterns of base cation concentrations in the forest litter and topsoil, and explore the effects of climate and tree species, we measured microclimate and collected the forest litter and topsoil(0-10 cm) samples across an elevational range of more than 2000 m(1243 ～ 3316 m a.s.l.),and analyzed the concentrations of BC in laboratory. Results showed that: 1) litter Ca concentration displayed a hump-shaped pattern along the elevational gradients, but litter K and Mg showed saddle-shaped patterns. Soil Ca concentration increased with elevation, while soil K and Mg had no significant changes. 2) Ca concentration in the forest litter under aspen(Populus davidiana) was significantly higher than that in all other species, but in topsoil, Ca concentration was higher under coniferous larch and fir(Larix chinensis and Abies fargesii). Litter K and Mg concentrations was higher under coniferous larch and fir, whereas there were nosignificant differences among tree species in the concentrations of K and Mg in topsoil. 3) Climatic factors including mean annual temperature(MAT), growing season precipitation(GSP) and non-growing season precipitation(NGSP) determined BC concentrations in the forest litter and topsoil. Soil C/N and C/P also influenced BC cycling between litter and soil. Observation along elevations within different tree species implies that above-ground tree species can redistribute below-ground cations, and this process is profoundly impacted by climate. Litter and soil Ca, K and Mg with different responses to environmental variables depend on their soluble capacity and mobile ability.     

农户对禁牧政策的行为响应及其影响因素研究——以新疆策勒县为例

以新疆策勒县为例,对当地农牧民在封育禁牧影响下的经济行为响应及其影响因素利用对数线性模型进行定量分析,以研究不同背景条件的农牧民在进行行为选择时的规律性因素。研究表明,农户所属区域及其拥有的资本水平是农户对禁牧行为响应的显著影响因素;农户所属区域及当地的技术服务培训等政策配套水平是农户对禁牧态度的显著影响因素;农牧民畜牧生产观念的转变和草场承包责任制的落实是实施禁牧政策的先决条件。建议政府针对区域及农户的资本差异进行分类补偿;当地政府可整合利用各相关部门的项目资源和资金进行禁牧的政策前期和推广工作。     

规则池塘群的复杂直线模式识别

针对现有面要素直线模式识别方法难以解决规则池塘中破碎区域的问题,提出了一种规则池塘群的复杂直线模式识别方法。首先,分析了规则池塘群的空间特征和认知特点,提出了“主次关系→并列关系→直线模式→复杂直线模式”的多层次认知顺序;其次,设计了主次关系池塘组、并列关系池塘组、直线模式池塘群的识别方法;最后,构建了复杂直线模式池塘群的识别模型。试验表明:本文方法能够消除破碎区域对规则池塘群直线模式提取的不利影响,有效提升复杂直线模式识别的质量。     

中国居民食物消费碳排放时空演变趋势及其驱动机制分析

食物生产是影响温室效应气体排放的重要方面,由于食物类型不同其碳排强度存在较大的差异,因此当前中国居民食物消费快速演替必然会对生态碳环境产生重要影响。论文利用洛伦兹曲线和基尼系数,研究1997—2016年中国城乡居民食物消费在生产过程中碳排放时空演变及其主要驱动因子。主要结论如下：① 在数量上,中国居民食物消费碳排放总量和人均量整体都呈现逐渐的增长刚性,但仍属于低碳消费模式;② 在结构上,各类型食物碳排放演变呈现显著的分异态势,整体上动物性食物碳排放增长速度快于植物性食物碳排放减少速度;③ 在城乡差异上,城镇和乡村居民食物消费碳排放在数量、结构和趋势上表现出显著的城乡二元属性,但近年城乡差异表现出减缓趋势;④ 在空间上,各地食物消费碳排放呈现较显著的民族性、地域性、集聚性和中心“塌陷”的特征,碳排放密度高值主要分布在直辖市和沿海经济相对发达地区;⑤ 在驱动力上,结构演替是全国居民食物消费碳排放总量整体变化的主要驱动因素,而人口数量是各地地域空间差异分布的主要驱动因子。     

霞水母刺丝囊毒素溶血活性的稳定性研究

从霞水母Cyanea sp.中分离刺丝囊,提取刺丝囊毒素(NV),并研究了温度、pH和几种化学组分对其溶血活性稳定性的影响.实验结果表明,在4～37℃范围内NV的溶血活性与温度变化密切相关,毒素在4℃以下几乎没有发生溶血反应,在37℃以下反应45 min以后,溶血活性达到最大值;毒素的溶血活性对pH敏感,在pH 7.8时.溶血活性最强,溶血率为93.53%.EDTA、NaCl和还原型谷胱甘肽(GSH)对NV溶血活性都具有稳定作用.通过正交实验发现,GSH对溶血活性的影响最大,当提取液中含有1.0 mmol/L GSH和5.0 mmol/L NaCl时毒素的溶血活性最强.DTT(二硫苏糖醇)明显降低毒素的溶血活性,说明毒素中溶血蛋白的活性很可能与分布在蛋白质表面的二硫键有关.Ca2+虽然能够增强毒素的溶血活性但是对毒素并没有稳定作用,所以Ca2+可能只是毒素溶血活性的活化剂.甘油对毒素的稳定作用不明显.