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301.
对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备 总被引:2,自引:0,他引:2
本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础. 相似文献
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SONG Peng TAN Jun LIU Zhaolun ZHANG Xiaobo LIU Baohua YU Kaiben LI Jinshan XIA Dongming XIE Chuang 《中国海洋大学学报(英文版)》2019,(4)
The gradient preconditioning approach based on seismic wave energy can effectively avoid the huge memory consumption of the gradient preconditioning algorithms based on the Hessian matrix. However, the accuracy of this approach is prone to be influenced by the energy of reflected waves. To tackle this problem, the paper proposes a new gradient preconditioning method based on the energy of transmitted waves. The approach scales the gradient through a precondition factor, which is calculated by the ‘approximate transmission wavefield' simulation based on the nonreflecting acoustic wave equation. The method requires no computing nor storing of the Hessian matrix and its inverse matrix. Furthermore, the proposed method can effectively eliminate the effects of geometric spreading and disproportionality in the gradient illumination. The results of model experiments show that the time-domain full waveform inversion(FWI) using the gradient preconditioning based on transmitted wave energy can achieve higher inversion accuracy for deep high-velocity bodies and their underlying strata in comparison with the one using the gradient preconditioning based on seismic wave energy. The field marine seismic data test shows that our proposed method is also highly applicable to the FWI of field marine seismic data. 相似文献
304.
LIU Wen-hui XIE Chang-wei ZHAO Lin WU Tong-hua WANG Wu ZHANG Yu-xin YANG Gui-qian ZHU Xiao-fan YUE Guang-yang 《山地科学学报》2019,(5)
Zonag, Kusai, Hedin Noel and Yanhu Lakes are independent inland lakes in the Hoh Xil region on the Qinghai-Tibet Plateau. In September2011, Zonag Lake burst after the water level had increased for many years. Floods flowed through Kusai and Hedin Noel Lakes into Yanhu Lake; since then, the four small endorheic catchments merged into one larger catchment. This hydrological process caused the rapid shrinkage of Zonag Lake and continuous expansion of Yanhu Lake. In this study,based on satellite images, meteorological data and field investigations, we examined the dynamic changes in the four lakes and analyzed the influencing factors. The results showed that before 2011, the trends in the four lake areas were similar and displayed several stages. The change in the area of Zonag Lake corresponded well to the change in annual precipitation(AP), but the magnitude of the change was less than that of a non-glacier-fed lake. Although increased precipitation was the dominant factor that caused Zonag Lake to expand, increased glacier melting and permafrost thawing due to climate warming also had significant effects. After the 2011 outburst of Zonag Lake, due to the increasing AP and accelerating glacier melting, the increases in water volume of the three lakes were absorbed by Yanhu Lake, and Yanhu Lake expanded considerably. According to the rapid growth rates in water level and lake area, Yanhu Lake is likely to burst in 1-2 years. 相似文献
305.
Ascertaining the relationship between "source-sink" landscape and non-point source(NPS) pollution is crucial for reducing NPS pollution, however, it is not easy to realize this target on cell unit scale. To reveal the relationships between "sourcesink" landscape and NPS pollution based on cell units of a small catchment in the Three Gorges Reservoir Region(TGRR), the runoff and nutrient yields were simulated first by rainfall events on a cell unit scale based on the Annualized AGricultural Non-Point Source Pollution Model(AnnAGNPS). Landscape structure and pattern were quantified with "sourcesink" landscape indicators based on cell units including landscape area indices and locationweighted landscape indices. The results showed that:the study case of small Wangjiagou catchment highlighted a good prediction capability of runoff and nutrient export by the AnnAGNPS model. Throughout the catchment, the spatial distribution trends of four location-weighted landscape indices were similar to the trends of simulated total nitrogen(TN) and total phosphorus(TP), which highlighted the importance of spatial arrangement of "source" and "sink" landscape types in a catchment when estimating pollutant loads. Results by Pearson correlation analysis indicated that the location-weighted landscape index provided a more comprehensive account of multiple factors, and can better reflect NPS-related nutrient loss than other landscape indices applied in single-factor analysis. This study provides new findings for applying the "source-sink" landscape indices based on cell units in small catchments to explain the effect of "source-sink" landscape on nutrient export based on cell unit, and helps improve the understanding of the correlation between "source-sink" landscape and NPS pollution. 相似文献
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In recent years, fish invasion has become one of the main reasons for the decline of nativefish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland(Lhasa, Tibet) during dif ferent seasons in2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P. parva. Furthermore, food relationships between P. parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P. parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013.The standard length(SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanf fy function was used to model the observed length-at-age data as Lt =112.19(1– e~(-0.1495(t +0.8012))) for females and as Lt =123.12(1– e~(-0.1500(t +0.7132))) for males. The results indicated that P. parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P. parva. P. parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o'connori, Schizopygopsis younghusbandi younghusbandi, and Ptychobarbus dipogon, for Bacillariophyta and Chironomid larvae.This is an important reason for the decline in native fish population. 相似文献
308.
Cultural tourism routes can reveal and protect cultural tourism heritage by means of cultural tourism in the “time category” and the “space category”. The construction of an evaluation system for cultural tourism routes and the evaluation, scoring and grading of existing and potential cultural tourism routes are the key to the protection of heritage, history and culture along these routes, and the key to encouraging the standardization, branding and sustainable development of various formats on the routes. Yunnan cultural tourism routes are diverse and rich in resources, and their developmental foundation is good. However, the tourism development and cultural development activities occur along separate lines and there is a lack of unified management. Therefore, it is necessary to conduct research on information collection, information analysis, problem diagnosis and improvement of status in the local practices of Yunnan cultural tourism routes, and to guide the sustainable development of Yunnan cultural tourism routes. Based on the advanced experience of COE (Council of Europe), UNESCO WHC (United Nations Educational, Scientific and Cultural Organization, World Heritage Committee) and ICOMOS(International Council on Monuments and Sites), this paper comprehensively analyzes the decisive criteria for line evaluation by interpreting and summarizing the connotations of cultural tourism routes, and determines that the evaluation of cultural tourism routes should include lines. Five aspects―the theme, the participating subject, the object itself, related activities and multi-party value―are used to construct a five-component model. At the same time, based on the consumer utility function, a complete route evaluation and hierarchical system is constructed. Then, taking the Ancient Tea-horse Road cultural tourism route as an example and using the expert scoring method and the analytic hierarchy process, the actual scores and grades of the Ancient Tea-horse Road cultural tourism route are determined, and countermeasures and suggestions for its sustainable development are proposed. The paper also verifies the applicability and practicability of the evaluation system, and promotes and improves the feedback evaluation system. The aim is to at promote and widely apply the evaluation system of cultural tourism routes, realizing the transformation from individual cases to joint cases, and promoting the standardization and sustainable development of cultural tourism routes. 相似文献
309.
2015年台风“彩虹”过境后下山冷急流引起的南海北部海域异常海面降温 总被引:1,自引:0,他引:1
This study deals with a unusual cooling event after Typhoon Mujigea passed over the northern South China Sea(SCS) in October 2015. We analyze the satellite sea surface temperature(SST) time series from October 3 to 18,2015 and find that the cooling process in the coastal ocean had two different stages. The first stage occurred immediately after typhoon passage on October 3, and reached a maximum SST drop of –2℃ on October 7 as the usual cold wake after typhoon. The second stage or the unusual extended cooling event occurred after 7d of the typhoon passage, and lasted for 5d from October 10 to 15. The maximum SST cooling was –4℃ and occurred after 12d of typhoon passage. The mechanism analysis results indicate that after landing and moving northwestward to the Yunnan-Guizhou Plateau(YGP), Typhoon Mujigea(2015) met the westerly wind front on October 5. The lowpressure and positive-vorticity disturbances to the front triggered meridional air flow and low-pressure trough,thus induced a katabatic cold jet downward from the Qinghai-Tibet Plateau(QTP) passing through the YGP to the northwestern SCS. The second cooling reached the maximum SST drop 4d later after the maximum air temperature drop of –9℃ on October 11. The simultaneous air temperature and SST observations at three coastal stations reveal that it is this katabatic cold jet intrusion to lead the unusual SST cooling event. 相似文献