南海东北部内潮的模态结构和传播

The evolution of energy, energy flux and modal structure of the internal tides(ITs) in the northeastern South China Sea is examined using the measurements at two moorings along a cross-slope section from the deep continental slope to the shallow continental shelf. The energy of both diurnal and semidiurnal ITs clearly shows a～14-day spring-neap cycle, but their phases lag that of barotropic tides, indicating that ITs are not generated on the continental slope. Observations of internal tidal energy flux suggest that they may be generated at the Luzon Strait and propagate west-northwest to the continental slope in the northwestern SCS. Because the continental slope is critical-supercritical with respect to diurnal ITs, about 4.6 kJ/m~2 of the incident energy and 8.7 kW/m of energy flux of diurnal ITs are reduced from the continental slope to the continental shelf. In contrast, the semidiurnal internal tides enter the shelf because of the sub-critical topography with respect to semidiurnal ITs.From the continental slope to the shelf, the vertical structure of diurnal ITs shows significant variation, with dominant Mode 1 on the deep slope and dominant higher modes on the shelf. On the contrary, the vertical structure of the semidiurnal ITs is stable, with dominant Mode 1.     

斑节对虾TSG101基因的克隆及表达分析

为探究肿瘤易感基因101(简称TSG101)对斑节对虾(Penaeusmonodon)的免疫应答作用,了解在细菌刺激下斑节对虾的机体发生的变化机制,本研究以哈维弧菌(Vibrioharveyi)和金黄色葡萄球菌(Staphylococcusaureus)为实验组,以磷酸缓冲液(PBS)为对照组,通过荧光定量分析展开对斑节对虾对菌刺激的免疫应答作用。结果显示,斑节对虾的TSG101在各组织中均有表达,在肝胰腺中的表达量最高。在金黄色葡萄球菌刺激下,斑节对虾的TSG101在肝胰腺中的表达量与对照组相比呈极显著上调(P0.01),第12小时的TSG101 mRNA的表达量达到最大(为对照组的21.60倍);在鳃中的表达量与对照组相比呈极显著上调(P0.01),第6小时斑节对虾TSG101的表达量达到最大值(为对照组的3.64倍)。在注射哈维弧菌第9小时,肝胰腺中的PmTSG101 mRNA表达量极显著上调(P0.01)且达到最大(为对照组的2.50倍)。实验结果初步表明,斑节TSG101参与斑节对虾的先天免疫反应,在金黄色葡萄球菌和哈维弧菌的刺激的情况下,该基因RNA水平的表达情况发生明显变化。     

加权EIV模型的经典最小二乘算法

采用GHM方法将EIV模型在最优解处线性化,得到解的近似方差。然后,将EIV模型表达成与Gauss-Markov模型相似的形式,利用标准最小二乘理论推导EIV模型的解及近似方差矩阵,得到与已有算法等价的结论。最后,推导观测值估值和残差的统计性质,建立起一整套EIV模型参数估计和精度评定的体系。     

Growth and feeding habits of invasive Pseudorasbora parva in the Chabalang Wetland(Lhasa,China) and its trophic impacts on native fish

In recent years, fish invasion has become one of the main reasons for the decline of nativefish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland(Lhasa, Tibet) during dif ferent seasons in2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P. parva. Furthermore, food relationships between P. parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P. parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013.The standard length(SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanf fy function was used to model the observed length-at-age data as Lt =112.19(1– e~(-0.1495(t +0.8012))) for females and as Lt =123.12(1– e~(-0.1500(t +0.7132))) for males. The results indicated that P. parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P. parva. P. parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o'connori, Schizopygopsis younghusbandi younghusbandi, and Ptychobarbus dipogon, for Bacillariophyta and Chironomid larvae.This is an important reason for the decline in native fish population.     

基于自发地理信息的人行道路信息提取

人类的行为轨迹可快速提取到车辆难以通行的人行道路以及人行道路设施等信息,这些信息是行人LBS的关键性元素,它的完备性程度决定了行人LBS服务质量的高低。本文使用志愿者数据集与百度地图,研究了一整套基于VGI数据的人行道路信息提取方法。通过轨迹数据清洗、道路几何路网提取、人行道路设施的检测与识别3个主要板块实现了人行道路信息的提取。算法在完成道路几何路网信息提取的同时,实现了人行横道、过街天桥、地下通道等道路设施信息的获取。     

近50 a来辽河口水下三角洲演变特征研究

本文基于近50 a来的辽河口海域的水下地形资料,采用GIS技术手段研究了辽河口水下三角洲的演变特征。研究结果表明:近50 a来辽河水下三角洲外缘(蛤蜊岗子滩西缘和南缘、西滩西缘和南缘)出现较大幅度的侵蚀后退;蛤蜊岗子滩东缘淤积外移,南部滩尾东部有北移东偏趋势;近岸浅滩由淤涨变为基本稳定,局部有所侵蚀后退;受蛤蜊岗子滩向东淤进的影响,蛤蜊岗子滩东侧深槽有所缩窄;蛤蜊岗子滩和西滩之间深槽水域出现拓宽变浅的变化趋势。流域来水来沙减少是近年来辽河口水下三角洲外缘侵蚀后退的主要原因,外缘侵蚀泥沙在波流作用下向河口搬运是蛤蜊岗子滩东侧淤积、深槽缩窄的主要原因,近岸边滩人工围垦对水下三角洲演变的影响值得关注。     

基于梯度依赖客观分析技术的全球Argo网格化数据集:构建及初步应用

采用梯度依赖相关尺度方法构建了1套2004—2017年间,月平均的全球海洋(0~1 500 m)1°×1°的Argo数据集,并在对该数据集进行对比检验的基础上,将其初步应用于中西太平洋黄鳍金枪鱼的渔场分析研究。结果表明,所构建的Argo数据集与WOA13数据集的温、盐偏差在上表层(150 m)稍大,最大值分别约为0.5 ℃和0.1,且偏差均随深度的增加而逐渐减小;其与TAO浮标时间序列的温度偏差,2004—2017年间均小于1 ℃,最大盐度偏差则小于0.5,且大部分海域接近0。中西太平洋海域,黄鳍金枪鱼中心渔场多集中在 28~29 ℃ 等温线范围内,在 22 ℃以下的海域单位捕捞努力量渔获量(catch per unit effort,CPUE)值极小;中心渔场区温跃层上界深度范围在20~120 m之间,且中心渔场在各个深度上形成的频数大体呈正态分布,温跃层上界深度为90 m时,形成中心渔场的可能性达到最大。研究表明所构建的数据集在水文环境分析及资源评估中有一定的应用价值。     

柳珊瑚Leptogorgia rigida中甾体类成分研究

首次对南海柳珊瑚Leptogorgia rigida中甾体类次生代谢产物进行研究。采用硅胶柱柱层析、十八烷基硅烷键合硅胶填料、Sephadex LH-20、高效液相色谱(HPLC)及薄层色谱(TLC)等多种方法进行分离纯化, 通过核磁共振波谱(NMR)、质谱(MS)等现代波谱分析及物理常数对照等方法进行结构鉴定。从南海柳珊瑚Leptogorgia rigida次生代谢产物中分离得到8个甾类化合物, 结构依次为: 3β, 6β, 11-trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (1); 3β, 6α, 11- trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (2); 3β, 6α, 11-trihydroxy-5α-chloest-7, 22(E)-dien-9-one (3); pregnan-4-en-3, 20-dione (4); 5α-pregnan-3, 20-dione (5); 3β-hydroxy-pregnan-5-en-20-dion (6); 3β-hydroxy-5α-pregnan-20-one (7); 和3β, 6α, 11-trihydroxy-9, 11-seco-5α-gorgosterol (8)。所有化合物均首次从Leptogorgia属中得到。     

单环刺螠soxb基因的克隆及其在幼虫发育中的表达特征

SOXB作为重要的转录因子,参与了动物内胚层分化、消化道形成、神经细胞和感官细胞分化的调控。本研究利用RACE技术克隆了单环刺螠(Urechis unicinctus)2个soxb亚族基因soxb1(Uu-soxb1)和soxb2(Uu-soxb2)的cDNA全长序列,大小分别是1871 bp和2906 bp。在两个推导的氨基酸序列中均包含SOX家族的HMG-box结构和SOXB亚族蛋白特有的Group B homology序列,进化树分析它们分别与SOXB1和SOXB2聚类。原位杂交结果显示,Uu-soxb1和Uu-soxb2为母源表达基因;二者在担轮幼虫中的表达图式存在差异,其中早期担轮幼虫中Uu-soxb1 mRNA广泛分布于虫体中,Uu-soxb2 mRNA则集中于顶纤毛束基部和虫体后部(口后纤毛环之后),中期担轮幼虫中,Uu-soxb2阳性信号主要位于纤毛环及虫体的下半球;而Uu-soxb1 mRNA主要位于虫体下半球;之后的发育中二者的表达位点基本一致,即:晚期担轮幼虫中主要位于虫体后部,体节幼虫和蠕虫状幼虫中主要分布于消化道和体壁处。本研究结果表明,单环刺螠soxb在消化道发育过程中的表达模式与其他已报道动物类似,提示其在消化道发育中具有保守的功能。