真鲷（Pagrus major）vasa基因5′端启动子克隆及表达载体构建

采用染色体步移（Genome walking）的方法克隆了真鲷vasa基因5′侧翼序列,采用生物信息学方法分析潜在的顺式作用元件,并与斑马鱼核心启动子进行比对,在此基础上构建了绿色荧光蛋白表达载体。序列分析结果显示：克隆得到的真鲷vasa基因5′侧翼序列序列长度为2762bp,其中包括TATA-box、CAAT-box...     

大菱鲆（Scophthalmus maximus）胚胎发育的形态学和组织学研究

采用显微镜和连续切片技术,观察了大菱鲆胚胎发育阶段的形态学和组织学特征。结果表明,大菱鲆胚胎发育主要经历6个时期;在14℃,胚体经108h即可孵化出膜。胚胎在64细胞期出现纬裂,分裂球分化为外层的包被层和内部的深层细胞;多细胞期形成卵黄合胞体层;低囊胚期形成囊胚腔。受精后26h30min胚盾出现。胚盘下包65%时,头突...     

Analysis of characteristics of time-frequency correlations between runoff, tidal range and salinity in the Changjiang Estuary

Power spectrum and cross-wavelet transform analysis was adopted to study the time-frequency characteristics and multiscale correlations between runoff, tidal range and salinity in the Changjiang Estuary based on the runoff data collected at the Datong Station, the tidal range measured at the Baozhen Station, and the salinity at the Baogang Station from 2008 to 2009. The variations of the salinity showed significant periodicity at scales of 2-3, 7-8, 14-15 and 26-30 d. The correlation between the salinity and the runoff and the tidal range were found to be significantly related to shock at scales of 5-7, 14-15, 26-30 d and 0.5 a. The correlation between the runoff and the salinity was mainly in the same phase, while the correlation between the tidal range and the salinity was in the antiphase. Different frequency bands were related to different degrees, and their relevance increased as the resonance frequency decreased. In addition, changes of the seasonal runoff were obvious. Specifically, a point of discontinuity was reached in early June with a cycle of 7-8 d, which coincided with the periodicity of plum rains in the Changjiang-Huaihe region. High-frequency changes (8-16 d period) of the salinity corresponded to the time domain in January-April 2008, February-April 2009 and October-December 2009 and exhibited an approximately 0.5 a (184 d) long frequency oscillation. Short-period changes were found to be stronger than long-period changes. Cross-wavelet transforms for the salinity, the runoff and the tidal range revealed local features in the time domain, while the significant levels of different periodic oscillations were observed in the frequency domain. The correlation characteristics of the salinity and the runoff were significant in the 80-90 d frequency domain, indicating that the major impact of the runoff on the salinity was reflected in seasonal changes. The tidal range on the small scale of 14-15 and 30-32 d was more obvious than the runoff.     

超压对碎屑岩机械压实作用的抑制与孔隙度预测

为了研究超压对碎屑岩储层机械压实作用的抑制作用和预测超压背景下碎屑岩储层的孔隙度,本文根据压力平衡原理,推导了地层超压与有效埋深的关系方程,并在综合考虑超压、溶蚀和胶结作用的基础上,建立了超压背景下储层孔隙度的预测模型。结果表明,盆地中地层的密度越小,超压对碎屑岩储层机械压实的抑制效果越明显。三个不同时代、不同类型沉积盆地的孔隙度预测结果表明,应用本文建立的储层孔隙度预测模型所预测储层孔隙度的误差均低于Gluyas和Cade方程,而且孔隙度预测的误差随地层时代的变老而增大。在储层胶结物小于10%的条件下,新生代超压盆地碎屑岩储层孔隙度的预测误差小于2%,而中生代超压盆地储层孔隙度的预测误差大于5%。本文建立的超压背景下的碎屑岩储层孔隙度预测模型仅适用于时代较新超压盆地的孔隙度预测。     

几株真菌对铊吸附作用的初步研究

铊是13种优先控制的有毒有害重金属元素之一,研究微生物与铊之间的相互作用有重要的理论意义和应用价值。在黔西南滥木厂铊矿区采集土壤和沉积物,借助平板筛选法在铊浓度为1000 mg/L水平筛选得到九株高耐受性菌株,用于真菌对铊的胞外吸附作用实验,并采用液体发酵法考察真菌对铊的胞外吸附作用,实验设计了1000 mg/L、1200 mg/L和1500mg/L三种铊处理水平,借助ICP-MS检测分析样品的铊含量,以此计算吸附效率。结果表明,三种处理水平中,真菌菌株对铊的吸附效率为4.63%~16.89%,且随着环境中铊浓度的上升,菌丝体(或菌丝球)生物量明显减少,导致吸附效率的下降;真菌对常量元素如钙、钠、钾的吸附与对铊的吸附呈显著正相关。这表明真菌细胞对钙、钾的吸附方式可能与对铊的吸附方式类似。     

莱州市土地管理办公自动化系统建设

莱州市国土资源局土地管理办公自动化系统采用工作流技术,基于浏览器/服务器（B/S）三层体系结构设计,实现了土地管理办公自动化和国土信息的对外发布。系统采用可视化流程设计,并预留GIS接口,方便今后系统的扩展和升级。系统建成后,大大提高了莱州市国土资源局各业务部门的业务处理速度和协同工作效率。     

海南石碌铁矿床构造变形特征及其与铁多金属成矿富集的关系

海南石碌铁矿曾被誉为“亚洲最大的富铁矿”,其形成、定位与褶皱变形及伴随的剪切、塑性流动等构造密切相关。石碌矿区构造变形大致分为早期(D1)复式向斜的形成时期、晚期(D2)褶皱叠加和剪切变形时期。后者又进一步分为韧-脆性变形(D2a),脆-韧性变形、层间滑脱断层形成(D2b)和脆性变形、矿体破坏(D2c)3个阶段。石碌铁矿床受NW—SE向复式向斜及其与NE—NNE向褶皱叠加所形成的横跨或斜跨褶皱的严格控制;褶皱过程所伴随的剪切变形和高温塑性流动是富铁矿形成的重要机制。复式向斜轴部,尤其是2期向斜褶皱轴的叠加部位往往可发现厚大的富铁矿体。     

燕山中段走滑断裂中辉绿岩脉的SHRIMP U-Pb年龄和Ar-Ar年龄

燕山中段东西向走滑断裂的活动时代一直存在争议,对侵入断层中的辉绿岩脉的直接测年可以给出一个年龄上限.侵入密云-喜峰口断裂中的辉绿岩脉的锆石SHRIMP U-Pb和角闪石Ar-Ar测年发现,锆石U-Pb年龄为290～125Ma,没有一个谐和年龄;角闪石Ar-Ar坪年龄为123.5Ma±1.5Ma.Ar-Ar年龄与最年轻的...     

甘肃中西部龙首山一带“韩母山群”的重新厘定

关于甘肃龙首山一带“韩母山群”的划分和时代归属,前人曾有不同的认识。依据沉积建造、古生物化石、变质变形等资料,对韩母山群进行解体。将该群下部变质变形较强的烧火筒沟组(钙质砾岩、细碎屑岩夹碳酸盐岩建造)厘定为震旦系,上部变质变形较弱的草大坂组(碎屑岩、碳酸盐岩建造)厘定为寒武系,并将后者与鄂尔多斯西缘下、中寒武统对比。     

迟缓爱德华氏菌OppA蛋白的免疫原性及免疫保护分析

迟缓爱德华氏茵(Edwardsiella tarda)是水产养殖动物重要的病原茵,引起鱼类爱德华氏茵病,免疫防治是针对该病的一个有效途径.OppA是迟缓爱德华氏茵的一个寡肽透过酶组分,作者开展OppA的免疫原性及免疫保护性的研究,旨在评价其作为鱼类爱德华氏菌病疫苗候选抗原的可行性.将致病性的迟缓爱德华氏菌LSE40的o...