Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR

Marine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5′-noncoding region (5′-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichiacoli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters.     

Bender Elements在测试土样剪切波速中的应用

本文以作者在香港理工大学土木与结构工程系设计制作的一套BenderElements为例,介绍了BenderElements的原理、发展历史、应用范围、使用方法和注意事项等。BenderElements是由压电陶瓷材料制成的,在拉伸、压缩作用下,会产生正、负电荷,反过来,给它施加正、负电荷后,它又可产生弯曲振动,正是利用它的这一特性,将其作成接收和发射装置,来测试土样的剪切波速Vs。BenderElements在安装使用时,防水和静电屏蔽十分关键。Vs可在场地地震反应、液化判别、动力基础设计、检测地基改良效果等方面获得应用。震相识别的原则是接收信号的第一个峰值为到时。     

Jovian temperature and cloud variability during the 2009-2010 fade of the South Equatorial Belt

Mid-infrared 7-20 μm imaging of Jupiter from ESO’s Very Large Telescope (VLT/VISIR) demonstrate that the increased albedo of Jupiter’s South Equatorial Belt (SEB) during the ‘fade’ (whitening) event of 2009-2010 was correlated with changes to atmospheric temperature and aerosol opacity. The opacity of the tropospheric condensation cloud deck at pressures less than 800 mbar increased by 80% between May 2008 and July 2010, making the SEB (7-17°S) as opaque in the thermal infrared as the adjacent equatorial zone. After the cessation of discrete convective activity within the SEB in May 2009, a cool band of high aerosol opacity (the SEB zone at 11-15°S) was observed separating the cloud-free northern and southern SEB components. The cooling of the SEBZ (with peak-to-peak contrasts of 1.0 ± 0.5 K), as well as the increased aerosol opacity at 4.8 and 8.6 μm, preceded the visible whitening of the belt by several months. A chain of five warm, cloud-free ‘brown barges’ (subsiding airmasses) were observed regularly in the SEB between June 2009 and June 2010, by which time they too had been obscured by the enhanced aerosol opacity of the SEB, although the underlying warm circulation was still present in July 2010. Upper tropospheric temperatures (150-300 mbar) remained largely unchanged during the fade, but the cool SEBZ formation was detected at deeper levels (p > 300 mbar) within the convectively-unstable region of the troposphere. The SEBZ formation caused the meridional temperature gradient of the SEB to decrease between 2008 and 2010, reducing the vertical thermal windshear on the zonal jets bounding the SEB. The southern SEB had fully faded by July 2010 and was characterised by short-wave undulations at 19-20°S. The northern SEB persisted as a narrow grey lane of cloud-free conditions throughout the fade process.The cool temperatures and enhanced aerosol opacity of the SEBZ after July 2009 are consistent with an upward flux of volatiles (e.g., ammonia-laden air) and enhanced condensation, obscuring the blue-absorbing chromophore and whitening the SEB by April 2010. These changes occurred within cloud decks in the convective troposphere, and not in the radiatively-controlled upper troposphere. NH₃ ice coatings on aerosols at p < 800 mbar are plausible sources of the suppressed 4.8 and 8.6-μm emission, although differences in the spatial distribution of opacity at these two wavelengths suggest that enhanced attenuation by a deeper cloud (p > 800 mbar) also occurred during the fade. Revival of the dark SEB coloration in the coming months will ultimately require sublimation of these ices by subsidence and warming of volatile-depleted air.     

The inversion of anelastic coefficient,source parameters and site respond using genetic algorithm

It gradually becomes a common work using large seismic wave data to obtain source parameters, such as seismic moment, break radius, stress drop, with completingof digital seismic network in China (Hough, et al, 1999; Bindi, et al, 2001). These parameters are useful on earthquake prediction and seismic hazard analysis.Although the computation methods of source parameters are simple in principle and the many research works have been done, it is not easy to obtain the parameters accurately. There are two factors affecting the stability of computation results. The first one is the effect of spread path and site respond on signal. According to the research results, there are different geometrical spreading coefficients on different epicenter distance. The better method is to introduce trilinear geometrical spreading model (Atkinson, Mereu, 1992; Atkinson, Boore, 1995; WONG, et al, 2002). In addition, traditional site respond is estimated by comparing with rock station, such as linear inversion method (Andrews, 1982), but the comparative estimation will introduce some errors when selecting different stations. Some recent research results show that site respond is not flat for rock station (Moya, et al, 2000; ZHANG,. et al, 2001; JIN, et al, 2000; Dutta, et al, 2001). The second factor is to obtain low-frequency level and corner frequency fromdisplacement spectrum. Because the source spectrum model is nonlinear function,these values are obtained by eye. The subjectivity is strong. The small change of corner frequency will affect significantly the result of stress drop.     

Patterns of Racial Diversity and Segregation in the United States: 1990–2010

The growing ethnic and racial diversity of the United States is evident at all spatial scales. One of the striking features of this new mixture of peoples, however, is that this new diversity often occurs in tandem with racial concentration. This article surveys these new geographies from four points of view: the nation as a whole, states, large metropolitan areas, and neighborhoods. The analysis at each scale relies on a new taxonomy of racial composition that simultaneously appraises both diversity and the lack thereof (Holloway, Wright, and Ellis 2012). Urban analysis often posits neighborhood racial segregation and diversity as either endpoints on a continuum of racial dominance or mirror images of one another. We disturb that perspective and stress that segregation and diversity must be jointly understood—they are necessarily related, although not as inevitable binary opposites. Using census data from 1990, 2000, and 2010, the research points to how patterns of racial diversity and dominance interact across varying spatial scales. This investigation helps answer some basic questions about the changing geographies of racialized groups, setting the stage for the following articles that explore the relationship between geography and the participation of underrepresented groups in higher education.     

PHYSICAL GEOGRAPHY IN SOUTHEAST ASIA: PAST THEMES, CURRENT STATUS AND FUTURE TRENDS

Our knowledge regarding the physical geography of Southeast Asia is incomplete to a surprising extent. This paper reviews the past research, describes the ongoing work, and attempts to identify the future trends. Coverage of such a large area, even for the last 25 years, requires study of publications in several languages and coping with literature which is not easily available. Therefore certain topics. which are prominent in current research and likely to remain so in the future were chosen and reviewed in detail. Such topics include studies related to active plate margin features; the Pleistocene in Southeast Asia; erosion and sedimentation rates; rainforest; river systems; karst in Southeast Asia; coastal geomorphology; and the urban environment. Environmental studies are rapidly gaining importance chiefly because of (a) the destruction of the natural vegetation and the associated loss of biodiversity and (b) the growing problems due to accelerated erosion and sedimentation This trend is likely to continue with accelerated destruction of forests, development of coastal areas, and urbanization of the landscape The direction of research in physical geography of Southeast Asia has been determined by a combination of individual research interest, governmental priorities, and international expectations     

Formation of iron plaque on mangrove roots receiving wastewater and its role in immobilization of wastewater-borne pollutants

Iron (Fe) plaque formed on mangrove root increased with wastewater discharge, but the extent was species-specific. For Bruguiera gymnorrhiza, Fe plaque concentration was 0.80 mg g(-1) root d.wt at Day 0 and increased to 4.59, 6.84 and 7.52 mg g(-1) at Day 75 in the fresh water control (FW), synthetic wastewater with pollutant concentrations five times of municipal sewage (5SW) and double of 5SW (10SW) treatments, respectively; the respective increases in Excoecaria agallocha were from 0.70 to 2.37, 10.73 and 13.21 mg g(-1). For Acanthus ilicifolius, similar increase was found in 5SW, but all of the plants were dead in 10SW at Day 75. The concentrations of heavy metals and phosphorus immobilized were positively correlated with the amounts of Fe plaque formed, but the regression coefficients varied among species. The performance of mangrove plants in wastewater treatments was related to the Fe plaque formed and its immobilized wastewater-borne pollutants.     

Seasonal changes in imposex and tissue burden of butyltin compounds in Thais clavigera populations along the coastal area of Mirs Bay, China

We assessed the current status of tributyltin (TBT) contamination of Thais clavigera (Gastropoda) along the coastal area of Mirs Bay, China for the first time. The snail samples were collected from 10 different sites in the summer (June) and winter (December) of 2006, respectively. They were analyzed for imposex status, i.e. relative penis size index (RPSI) and vas deferens sequence index (VDSI), followed by quantification of butyltins in their tissues. Most of the collected females suffered from imposex, but the level of butyltin contamination varied with the distance from Yantian Port, which is currently the fourth busiest container port in the world. The tissue concentration of TBT varied with season. For a particular site, the winter samples in general contained much higher concentration of TBT than the summer samples. RPSI, VDSI and organotin concentrations were higher in T. clavigera collected from sites closer to the Port, such as the Seafood Street and Kat O. Both RPSI and VDSI were positively correlated with the tissue burden of tributyltin. VDSI exhibited little seasonal variability, whereas RPSI showed marked seasonal variability, with lower values in the summer samples. The current results will serve as an important reference for long-term monitoring of butyltin contamination in this area.     

Winkler's method overestimates dissolved oxygen in seawater: Iodate interference and its oceanographic implications

Dissolved oxygen in seawater has been determined by using the Winkler's reaction scheme for decades. An interference in this reaction scheme that has been heretofore overlooked is the presence of naturally occurring iodate in seawater. Each mole of iodate can result in an apparent presence of 1.5 mol of dissolved oxygen. At the concentrations of iodate in the surface and deep open ocean, it can lead to an overestimation of 0.52 ± 0.15 and 0.63 ± 0.05 μmol kg^− 1 of oxygen in these waters respectively. In coastal and inshore waters, the effect is less predictable as the concentration of iodate is more variable. The solubility of oxygen in seawater was likely overestimated in data sources that were based on the Winkler's reaction scheme for the determination of oxygen. The solubility equation of García and Gordon [Garcia H.E., Gordon, L.I., 1992. Oxygen solubility in seawater: Better fitting equations. Limnol. Oceanogr. 37, 1307–1312] derived from the results of Benson and Krause [Benson, F.B., Krause, D. Jr., 1984. The concentration and isotopic fractionation of oxygen dissolved in freshwater and seawater in equilibrium with the atmosphere. Limnol. Oceanogr. 29, 620–632] is free from this source of error and is recommended for general use. By neglecting the presence of iodate, the average global super-saturation of oxygen in the surface oceans and the corresponding efflux of oxygen to the atmosphere both have been overestimated by about 8%. Regionally, in areas where the degree of super-saturation or under-saturation of oxygen in the surface water is small, such as in the tropical oceans, the net air–sea exchange flux can be grossly under- or overestimated. Even the estimated direction of the exchange can be reversed. Furthermore, the presence of iodate can lead to an overestimation of the saturation anomaly of oxygen in the upper ocean attributed to biological production by 0.23 ± 0.07%. AOU may have been underestimated by 0.52 ± 0.15 and 0.63 ± 0.05 μmol kg^− 1 in the surface mixed layer and deep water, while preformed phosphate and preformed nitrate may have been overestimated by 0.004 ± 0.001 and 0.06 ± 0.02 μmol kg^− 1 in the surface mixed layer, and 0.005 ± 0.0004 and 0.073 ± 0.006 μmol kg^− 1 in the deep water. These are small but not negligible corrections, especially in areas where the values of these parameters are small. At the increasing level of sophistication in the interpretation of oxygen data, this source of error should now be taken into account. Nevertheless, in order to avoid confusion, an internationally accepted standard needs to be adopted before these corrections can be applied.