Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

基于参数空间分布的海洋生态系统模拟

在模拟大尺度海洋生态系统时,由于子区域的生态系统有着各自的特征,导致参数值在空间上存在差异,因此参数在整个研究区域取常数的做法必须改进.基于此,使用气候模式FOAM的气侯态背景场驱动一个简单的三维海洋生态系统模型,并引入参数的空间分布,在全球尺度上通过伴随方法同化SeaWiFS叶绿素资料.引入参数空间分布后,同化结果得到很大改进:浮游植物表层生物量(氮)的平均差从0.155 3减小至0.060 6 mmol·m-3,下降了60.9%,有效地降低了模拟值与观测值在空间上的差异;浮游植物表层生物量平均值也从0.103 1上升至0.125 2 mmol·m-3,更接近SeaWiFS观测.实验结果表明通过引入参数的空间分布来改进海洋生态系统的模拟是可行的.     

三种验潮方法水位观测性能比较与统计分析

水尺、压力式验潮仪与验潮井验潮仪是海道测量水位控制和水位观测的主要手段。通过6日6个时间段的同步比对试验,计算了三种方法获取水位观测数据之间的潮时差和潮高差,分析了三种观测手段的水位观测性能以及产生差值的可能原因,提出了使用方法的建议。     

不同投喂方式对中国明对虾幼体生长发育及抗病力的研究

研究β-1,3/1,6-D-glucan及不同投喂方式对中国明对虾(Fenneropenaeus chinensis)幼体的存活、生长、发育、非特异性免疫及抗副溶血弧菌能力的影响.将β-glucan以0.25%的剂量添加到微黏合饲料中,以不同的投喂方式投喂对虾幼体,即D0(一直投喂基础饲料);D1(一直投喂含0.25%β-葡聚糖饲料);3D1-3D0(3 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料);1D1-3D0(1 d投喂含0.25%β-葡聚糖的饲料-3 d投喂基础饲料),共4个处理,每个处理随机设置3个重复,对虾幼体养殖于可控温的半开放系统中,每个养殖桶中盛有50 L盐度为30～32消毒海水,养殖密度为100尾/L.实验结果表明,在孵化后14 d,投喂D1饲料组在总存活率,变态指数,体长,特定生长率方面均高于其它各组,虽然有时差异不显著.在免疫指标方面,在9和14 dph分别进行了酚氧化酶(PO)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力的测定,结果表明,投喂D1饲料组的4个免疫指标均高于其它3组,虽然有时差异不显著.在14 dph的攻毒实验结果表明,对照组的累积死亡率显著高于D1组(P<0.05),而1D1-3D0和3D1-3D0的累积死亡率则与其它各组均不显著.实验结果表明,间隔投喂方式并不适合于对虾幼体,在中国明对虾幼体饲料中使用β-葡聚糖时,建议添加量为0.25%,并采用一直投喂的方式.     

饲料中不同糖源对皱纹盘鲍体脂组成的影响

采用单因素实验设计研究饲料中分别添加6种不同糖源(糊精、糊化小麦淀粉、小麦淀粉、玉米淀粉、木薯淀粉和马铃薯淀粉)对皱纹盘鲍甘油三酯、胆固醇及脂肪酸含量的影响.实验共设6个处理,每个处理设3个重复,每个重复放养30只鲍,壳长:(29.98±0.09)mm,体重:(3.42±0.02)g;在水温18～20 ℃的循环水系统中进行为期24周的养殖实验.结果表明,摄食糊精、糊化小麦淀粉和小麦淀粉组鲍血清甘油三酯含量显著高于玉米淀粉组(P<0.05),摄食糊精和糊化小麦淀粉组鲍血清胆固醇含量显著高于玉米淀粉组(P<0.05).摄食玉米淀粉组鲍肝胰脏中饱和脂肪酸C16∶0含量显著低于小麦淀粉、木薯淀粉和马铃薯淀粉组(P<0.05);肌肉和肝胰脏中饱和脂肪酸C18∶0含量在摄食糊精组鲍中最低;肌肉C18∶3n-3含量在摄食木薯和马铃薯淀粉组鲍中最低并显著低于其他饲料组(P<0.05),而肝胰脏C18∶3n-3含量则在摄食糊精和糊化小麦淀粉组鲍中最低;摄食糊精组鲍肌肉C20∶4n-6含量显著低于小麦淀粉、玉米淀粉、木薯淀粉和马铃薯淀粉组(P<0.05),摄食糊化小麦淀粉组鲍肝胰脏C20∶4n-6含量显著低于玉米淀粉、木薯淀粉和马铃薯淀粉组(P<0.05);肌肉C22∶6n-3含量在摄食糊精和糊化小麦淀粉组鲍中显著低于小麦淀粉和马铃薯淀粉组(P<0.05).总体来说,皱纹盘鲍摄食结构相对简单的糖源(糊精、糊化小麦淀粉和小麦淀粉)能够增加血清甘油三酯和胆固醇的含量,以淀粉为来源的多糖(小麦淀粉、玉米淀粉、木薯淀粉和马铃薯淀粉)使鲍体多不饱和脂肪酸含量升高.     

Detection of nighttime sea fog/stratus over the Huanghai Sea using MTSAT-1R IR data

A dual channel difference (DCD) method is applied to detect nighttime sea fog/stratus over the Huanghai Sea using the infrared (IR) data of shortwave (3.5–4.0 μm) and longwave (10.3–11.3 μm) channels from the Multi-functional Transport Satellite (MTSAT)-1R, i.e., shortwave minus longwave brightness temperature difference (SLTD). Twenty-four sea fog events over the Huanghai Sea during March to July of 2006 and 2007 are chosen to determine a suitable value of SLTD for nighttime sea fog/stratus detection, and ...     

牙鲆受精的细胞学研究

通过组织切片,对牙鲆(Paralichthys olivaceus)的受精过程进行细胞学观察发现,这一过程包括精子入卵、卵母细胞第二次成熟分裂排出第二极体、雌雄原核形成与融合,直到合子进行第一次有丝分裂,在受精的整个过程中所有观察过的受精卵都只发现一个精子形成原核,并最终与卵原核结合形成合子,由此判断牙鲆的受精方式是单精受精.本实验的研究结果,不仅能够从理论上丰富海水鱼类的受精生物学基础研究资料,又可为生产中牙鲆染色体操作遗传育种提供依据.     

雨生红球藻八氢番茄红素脱氢酶pds基因上游序列的分离和分析

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5’上游序列。通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE调控元件、C-repeat/DRE调控元件等。同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测。结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达。     

2004 年夏初、秋末长江口外海区冲淡水及羽状锋的盐度特征

用国家海洋局东海环境监测中心2004年夏初（6月）和秋末（11月）对长江口外海区现场观测资料,用Matlab计算机编程语言对观测资料进行质量控制、插值网格化,绘制研究海区盐度立体分布图,显示夏初、秋末两季的盐度分布特征。将2004年夏、秋两季长江口外海区冲淡水和羽状锋的分布与以往观测研究结果对比,可得：（1）长江口外海区冲淡水的盐度值主要在5—31之间,冲淡水前缘盐度锋面的盐度值为18—28;盐度值20—28的盐度锋面基本体现该海区羽状锋的特征及变化。（2）夏初长江口外海区冲淡水范围明显大于秋末,夏初该海区以冲淡水为主,秋末冲淡水的范围收缩,紧贴沿岸一带。相应地,夏初该海区羽状锋呈倾斜锋面,占据较大的海区;秋末则为垂直锋面,介于122．50&#176;E-122．70&#176;E海区。     

Environmental evolution of Gongwang Mountains during Holocene in northeastern Yunnan, China

This paper presents the study on two continuous glacio-lacustrine sedimentary deposits, Laotanfang （3 597 m） and Shibantang （3 689 m） located on the southern and northern sides of Hulifang peak in Yunan, China. Environmental change indexes are constructed for each deposit, based on grain size, geochemical and pollen analysis, environmental magnetic susceptibility, and 14C dating. The study of climatic environmental change since the Holocene epoch in this region shows that the climate fluctuated at different stages during the early Holocene, that is, the climate was cool and wet during 8.4-7. 7r ka, cold and wet during 7.7-6. 5 ka, cold and dry during 6. 5-4.7 ka, cool and wet during 4.7-2. 1 ka, warm and wet during 2. 1-1.0 ka, and warmer and wet since 1 ka. These findings reflect the overlapping influences of the southwest and southeast monsoons in different times during the Holocene in the north part of southwestern China are in accord with other Holocene climate change models in this part of China.