Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

Second-Order Wave Diffraction Around 3-D Bodies by A Time-Domain Method

A time-domain method is applied to simulate nonlinear wave diffraction around a surface piercing 3-D arbitrary body. The method involves the application of Taylor series expansions and the use of perturbation procedure to establish the corresponding boundary value problems with respect to a time-independent fluid domain. A boundary element method based on B-spline expansion is used to calculate the wave field at each time step, and the free surface boundary condition is satisfied to the second order of wave steepness by a numerical integration in time. An artificial damping layer is adopted on the free surface for the removal of wave reflection from the outer boundary. As an illustration, the method is used to compute the second-order wave forces and run-up on a surface-piercing circular cylinder. The present method is found to be accurate, computationally efficient, and numerically stable.     

南麂列岛潮间带环境本底调查

本文系根据１９９２年８月和１９９３年２月（夏、冬两季）对南麂列岛潮间带的环境本底调查以及对水质、底质、生物样监测结果的资料整理而成。调查结果表明，潮间带水质、底质除个别断面外，均处于良好状态。生物体重金属残留量全部低于全国海岸带调查时统一的标准，生态环境条件较好。     

Role of mangrove in mercury cycling and removal in the Jiulong Estuary

Baseline of mercury in mangrove swamp The mercury content in sediments in the Jiulong Estuary was determined in 1984 and 1985(Table 1). The highest content of mercury in sediments (300×10~(-9)) was found in Ditou, contrast-ing strongly with the low content of 36×10~(-9) in Port Dongzhai in the Hainan Island. Tab1e 1 showsthat the average content of mercury in sediments in the Jiulong Estuary is 140×10~(-9) and the averageof four areas is 72×10~(-9). This value means that these areas have not been heavily contaminated by     

中国陆架海绿石分布特征及其意义

本文根据实际资料,简要概括了中国大陆架海绿石的分布规律和形成环境。海绿石的富集区与氧化环境,粗粒沉积和残留沉积区相一致。该研究为恢复古沉积环境提供了依据。     

一种腐屑食性鱼类对生态系统氮循环的影响

研究表明 :腐屑食性鱼类——梭鱼对环境中有机氮的摄入能力随体重的增加而增加 ,随温度的升高 ,体重系数显著减少。摄入氮 (CN,mg/ d)与体重 (W,g)和温度 (T,℃ )的数值关系可用如下模型表示 :CN=0 .6 370 W1 .0 642 ln(3.70 0 8T)。体重为 1 0 0 g的梭鱼每日可消耗环境中 332 mg氮有机质。梭鱼氮的吸收效率主要受温度的影响 ,而氮的转化效率与体重呈显著的正相关关系。随体重的增加 ,排泄氮所占比例降低     

福建红树林区海藻的分布及季节变化

主要研究了福建省3个红树林区的海藻不同滩面分布的情况、海藻种类组成的季节变化以及不同季节的海藻群落类型.研究表明:(1)福建红树林区海藻在不同滩面分布表现出,红藻较喜荫蔽潮湿的环境,而绿藻适生在光照条件较好生境.(2)由福建红树林区各门海藻种类组成的季节变化来看,蓝藻种数在一年四季没有明显变化规律,红藻四季种数变化幅度不大,而绿藻在不同季节种数有明显变化,春季种数最多,进入夏季后种数逐渐减少,到秋、冬季后种数又开始上升,到第2年春季又达到最多.(3)不同季节福建红树林区海藻群落类型中,优势种主要是红藻,以及一些绿藻.     

Comparison of Drags on Fish Cages of Different Shapes

1 .Introduction Commercial fishfarming in net cages is becoming widespread around the world as an importantsource of food.In PRChina ,mostfishfarmingtakes placeincalmcoastal areas .Fishcages work wellat these sites becausethe environmental forces are usua…     

转“全鱼”溶菌酶基因大菱鲆的研究

用大西洋条鳕(Macrozoarces americanus)抗冻蛋白启动子opAFP promoter和牙鲆(Paralichthys olivaceus)c型溶菌酶基因构建“全鱼”溶菌酶基因元件opAFP—ly，首次应用电脉冲精子介导法将该基因片段导入到大菱鲆(Scophthalmus maximus)受精卵内，获得原代转基因大菱鲆。先后采用单因子方法和正交实验方法，重复实验确定了最适导入条件：脉冲电压为400V／cm，脉冲次数为5，脉冲宽度为25ms，外源DNA浓度为50mg/L。利用最适导入条件，实现基因元件大规模转移。采用PCR技术对1月龄的原代转基因大菱鲆仔鱼的染色体DNA进行分析，结果表明外源基因的整合率可达28％。     

酸雨的研究进展及厦门酸雨研究的趋势

本文综述了国内外酸雨的研究现状；包括酸雨的来源、成因、模式、影响及防治对策；介绍了酸雨的研究趋势；并着重就厦门地区今后酸雨的研究方向提出也作者的看法。