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151.
Spores of Bacillus subtilis were used as a model system to study the impact of the two major DNA double-strand break (DSB) repair mechanisms [homologous recombination (HR) and non-homologous end-joining (NHEJ)] on the survivability of air-dried mono- and multilayers of bacterial spores under a simulated martian environment; i.e., an environment with low temperature (−10 °C), pure CO2 atmosphere (99.99% CO2), 200-1100 nm UV-VIS-NIR radiation, and 0.69 kPa pressure. Spores in multilayers exhibited low inactivation rates compared to monolayers, mainly due to shadowing effects of overlying spores. Simulated martian UV irradiation reduced dramatically spore viability, whereas when shielded from martian UV radiation, spores deficient in NHEJ- and HR-mediated DNA repair were significantly more sensitive to simulated martian environmental conditions than were wild-type spores. In addition, NHEJ-deficient spores were consistently more sensitive than HR-deficient spores to simulated Mars environmental conditions, suggesting that DSBs were an important type of DNA damage. The results indicated that both HR and NHEJ provide an efficient set of DNA repair pathways ensuring spore survival after exposure to simulated martian environmental conditions. 相似文献
152.
Analytical Expression for Vertical Stress within an Inclined Mine Stope with Non-parallel Walls 总被引:1,自引:0,他引:1
Ching Hung Ting Nagaratnam Sivakugan Wayne Read Sanjay Kumar Shukla 《Geotechnical and Geological Engineering》2014,32(2):577-586
Arching is a phenomenon that occurs in many situations in geotechnical engineering. When underground mine stopes are backfilled, a significant fraction of the self-weight of the backfill is carried by the side walls. As a result, the vertical stress at the bottom of the stope is significantly less than its overburden pressure. Few analytical expressions published in the literature can be used to determine the vertical stresses of stope with parallel walls. The objective of this paper is to extend the analytical solution previously developed by the authors to long plane-strain stopes with non-parallel walls with both slopes leaning to the same side. Different combinations of wall inclination are examined using the new analytical expression developed. To validate the analysis, the proposed results are compared with numerical model results. The results show that the proposed analytical expression is capable of estimating the vertical stress within mine stopes when the inclination of the hangingwall to the horizontal (α) is less than that of footwall (β). An important behavioural trend for the stress distribution is observed, where with the same overburden pressure and base width, the stress magnitude experienced by fill material significantly varies depending on the wall inclination. 相似文献
153.
Abstract This study reports on tower measurements from the intertidal zone taken during the ice‐free period between August 1 and September 20, 1985. Sea and air temperatures showed ranges of 8 and 14°C, respectively, and both were colder during onshore than during offshore winds. Onshore winds were associated with a nearly saturated atmosphere whereas offshore ones were quite dry. Surface albedo was twice as great for low tide as for high tide. The ratio net/solar radiation was 13% less at low tide owing to both the larger albedo and the stronger long‐wave radiation loss. Heat fluxes into the bottom sediments were small with net gains in August and net losses in September. During the day, heat storage in the water was large and positive. This occurred even with the tide out, when the ponded water continued to warm. At night the water gave up heat, both for low and high tide, and especially late in the season. The latent heat flux was always positive and was largest by day during low tide and by night during high tide. The sensible heat flux was positive for onshore winds and often negative for offshore winds. Under all wind directions heat storage constituted 60% of net radiation, the latent heat flux 35% and the remainder was proportioned equally between the sensible heat flux and the flux into the bottom sediments. 相似文献
154.
Wayne R. Rouse 《大气与海洋》2013,51(2):346-366
Abstract This study treats the energy balance during fast‐ice and floating‐ice conditions and examines overall seasonal patterns. The rate of ablation of the fast ice was controlled equally by net radiation and air temperature. The ratio of net/solar radiation increased 2.5 times during the ablation period owing to the decrease in ice albedo. Air temperature in the ablation zone was up to 8°C colder than that over the adjacent snow‐free terrestrial surface and remained near 0°Cfor the full ablation period. The sensible heat flux was small and downward (negative), whereas the evaporative heat flux was small and positive. Thus, the energy used in melting the ice was approximately equal to that provided by the net radiation. Above‐freezing air temperatures decreased the albedo through surface melting thus increasing net radiation. This combination of higher temperature and large net radiation was associated with offshore winds and resulted in large ablation relative to periods with colder onshore winds. The floating‐ice period is one of great variability owing to changing ice conditions, variable current behaviour, tidal cycles and changing wind direction. The intertidal zone acts as a major heat sink, both early and late in the floating‐ice period. The turbulent heat fluxes were small and were either positive or negative. Nearly all of the energy from net radiation was used in melting ice and in warming tidal water during high tide and in warming the residual tidal ponds and in melting stranded ice rafts during low tide. The overall study period, from May to September, included most of the season of positive radiation balance and above‐freezing temperatures. Winds were dominantly onshore in the first half of the period and equally onshore and offshore in the second half. Wind frequencies resembled longer term averages for other stations on James Bay and Hudson Bay. The ratio of net to solar radiation was at a maximum during the ice‐free period in August, whereas for adjacent terrestrial surfaces, it was largest at the summer solstice. Land‐sea breezes first developed in mid‐July and were influential in making offshore winds the dominant nocturnal regime. As a result, offshore winds were associated with small magnitudes of net radiation. Onshore winds were more than 5°C colder than those blowing offshore and their vapour pressure deficits were three times smaller. Convective heat fluxes were small for onshore winds and very small and usually negative for offshore winds. For all wind directions throughout the period, most of the available radiant energy was used to melt ice and to heat the sea water. This is a pattern similar to that of the ice‐covered or open sea and dissimilar to that of the adjacent terrestrial environment. It implies that the main energy‐balance transitions, during onshore airflow, occur at the high‐tide line. 相似文献
155.
Jordi LLORCA Josep M. TRIGO‐RODRÍGUEZ Jos L. ORTIZ Jos A. DOCOBO Javier GARCÍA‐GUINEA Alberto J. CASTRO‐TIRADO Alan E. RUBIN Otto EUGSTER Wayne EDWARDS Matthias LAUBENSTEIN Ignasi CASANOVA 《Meteoritics & planetary science》2005,40(6):795-804
Abstract— An impressive daylight fireball was observed from Spain, Portugal, and the south of France at 16h46m45s UTC on January 4, 2004. The meteoroid penetrated into the atmosphere, generating shock waves that reached the ground and produced audible booms. The associated airwave was recorded at a seismic station located 90 km north of the fireball trajectory in Spain, and at an infrasound station in France located 750 km north‐east of the fireball. The absolute magnitude of the bolide has been determined to be ?18 ± 1 from a casual video record. The energy released in the atmosphere determined from photometric, seismic, and infrasound data was about 0.02 kilotons (kt). A massive fragmentation occurred at a height of 28 ± 0.2 km, resulting in a meteorite strewn field of 20 × 6 km. The first meteorite specimen was found on January 11, 2004, near the village of Villalbeto de la Peña, in northern Palencia (Spain). To date, about 4.6 kg of meteorite mass have been recovered during several recovery campaigns. The meteorite is a moderately shocked (S4) L6 ordinary chondrite with a cosmic‐ray‐exposure age of 48 ± 5 Ma. Radioisotope analysis shows that the original body had a mass of 760 ± 150 kg, which is in agreement with the estimated mass obtained from photometric and seismic measurements. 相似文献
156.
Michael Zemcov Mark Halpern Colin Borys Scott Chapman Wayne Holland Elena Pierpaoli Douglas Scott 《Monthly notices of the Royal Astronomical Society》2003,346(4):1179-1188
We have detected the Sunyaev–Zel'dovich (SZ) increment at 850 μm in two galaxy clusters (Cl 0016+16 and MS 1054.4−0321) using the Submillimetre Common User Bolometer Array (SCUBA) on the James Clerk Maxwell Telescope. Fits to the isothermal β model yield a central Compton y parameter of (2.2 ± 0.7) × 10−4 and a central 850-μm flux of Δ I 0 = 2.2 ± 0.7 mJy beam−1 in Cl 0016. This can be combined with decrement measurements to infer y = (2.38 ±0.36 0.34 ) × 10−4 and v pec = 400±1900 1400 km s−1 . In MS 1054 we find a peak 850-μm flux of Δ I 0 = 2.0 ± 1.0 mJy beam−1 and y = (2.0 ± 1.0) × 10−4 . To be successful such measurements require large chop throws and non-standard data analysis techniques. In particular, the 450-μm data are used to remove atmospheric variations in the 850-μm data. An explicit annular model is fit to the SCUBA difference data in order to extract the radial profile, and separately fit to the model differences to minimize the effect of correlations induced by our scanning strategy. We have demonstrated that with sufficient care, SCUBA can be used to measure the SZ increment in massive, compact galaxy clusters. 相似文献
157.
Impacts of Land Process on the Onset and Evolution of Asian Summer Monsoon in the NCEP Climate Forecast System 总被引:1,自引:0,他引:1
Impacts of land models and initial land conditions(ICs) on the Asian summer monsoon,especially its onset,were investigated using the NCEP Climate Forecast System(CFS).Two land models,the Oregon State University(OSU) land model and the NCEP,OSU,Air Force,and Hydrologic Research Laboratory(Noah) land model,were used to get parallel experiments.The experiments also used land ICs from the NCEP/Department of Energy(DOE) Global Reanalysis 2(GR2) and the Global Land Data Assimilation System(GLDAS).Previous studies have demonstrated that,a systematic weak bias appears in the modeled monsoon,and this bias may be related to a cold bias over the Asian land mass.Results of the current study show that replacement of the OSU land model by the Noah land model improved the model’s cold bias and produced improved monsoon precipitation and circulation patterns.The CFS predicted monsoon with greater proficiency in El Nin o years,compared to La Nin a years,and the Noah model performed better than the OSU model in monsoon predictions for individual years.These improvements occurred not only in relation to monsoon onset in late spring but also to monsoon intensity in summer.Our analysis of the monsoon features over the India peninsula,the Indo-China peninsula,and the South Chinese Sea indicates different degrees of improvement.Furthermore,a change in the land models led to more remarkable improvement in monsoon prediction than did a change from the GR2 land ICs to the GLDAS land ICs. 相似文献
158.
Xiao Lin Mark J. McCarthy Stephen A. Carini Wayne S. Gardner 《Continental Shelf Research》2011,31(2):120-128
A new approach, combining 15NH4+ isotope dilution and continuous-flow techniques, provided estimates of “actual” and “net” NH4+ flux and sediment NH4+ demand (SAD) at the sediment-water interface (SWI) of sites in the northern Gulf of Mexico (NGOMEX). The sites included a hypoxic site (C6), two sites with intermediate oxygen levels (B7 and F5), and a normoxic site (CT). Control cores without isotope addition and other cores from the same site treated with 15NH4+ labeled overflowing water differentiated between net and actual regeneration flux and actual vs. potential uptake flux of NH4+. Experiments were conducted in 2008 before (July) and after (September) two successive hurricanes (Gustav and Ike) and in January and August, 2009. Actual regeneration was significantly higher than net flux at most sites. Net flux did not differ significantly in most sites/dates, but the actual regeneration, and the actual and potential uptake, showed temporal and spatial variation; the flux at the hypoxic site was more active than non-hypoxic sites. SAD, the difference between potential and actual NH4+ uptake flux, was higher at the hypoxic site than at non-hypoxic sites before and after the hurricanes in 2008 and during the hypoxia season in 2009. SAD related negatively to bottom water DO values. Conclusions: (1) net flux often underestimated actual regeneration, (2) hurricane activity decreased N dynamics, and (3) microbial N limitation status at the hypoxic site related to NH4+ removal processes that were independent of oxygen (e.g., anaerobic heterotrophic uptake or anammox). These results indicate a rather consistent NH4+ demand at the SWI during the hypoxic season and suggest that reduced nitrogen may limit microbial dynamics in the region. 相似文献
159.
Geochemistry of hydrothermal fluids from the PACMANUS, Northeast Pual and Vienna Woods hydrothermal fields, Manus Basin, Papua New Guinea 总被引:3,自引:0,他引:3
Eoghan P. Reeves Jeffrey S. Seewald Wolfgang Bach Wayne C. Shanks Emily Walsh Martin Rosner 《Geochimica et cosmochimica acta》2011,75(4):1088-102
Processes controlling the composition of seafloor hydrothermal fluids in silicic back-arc or near-arc crustal settings remain poorly constrained despite growing evidence for extensive magmatic-hydrothermal activity in such environments. We conducted a survey of vent fluid compositions from two contrasting sites in the Manus back-arc basin, Papua New Guinea, to examine the influence of variations in host rock composition and magmatic inputs (both a function of arc proximity) on hydrothermal fluid chemistry. Fluid samples were collected from felsic-hosted hydrothermal vent fields located on Pual Ridge (PACMANUS and Northeast (NE) Pual) near the active New Britain Arc and a basalt-hosted vent field (Vienna Woods) located farther from the arc on the Manus Spreading Center. Vienna Woods fluids were characterized by relatively uniform endmember temperatures (273-285 °C) and major element compositions, low dissolved CO2 concentrations (4.4 mmol/kg) and high measured pH (4.2-4.9 at 25 °C). Temperatures and compositions were highly variable at PACMANUS/NE Pual and a large, newly discovered vent area (Fenway) was observed to be vigorously venting boiling (358 °C) fluid. All PACMANUS fluids are characterized by negative δDH2O values, in contrast to positive values at Vienna Woods, suggesting substantial magmatic water input to circulating fluids at Pual Ridge. Low measured pH (25 °C) values (∼2.6-2.7), high endmember CO2 (up to 274 mmol/kg) and negative δ34SH2S values (down to −2.7‰) in some vent fluids are also consistent with degassing of acid-volatile species from evolved magma. Dissolved CO2 at PACMANUS is more enriched in 13C (−4.1‰ to −2.3‰) than Vienna Woods (−5.2‰ to −5.7‰), suggesting a contribution of slab-derived carbon. The mobile elements (e.g. Li, K, Rb, Cs and B) are also greatly enriched in PACMANUS fluids reflecting increased abundances in the crust there relative to the Manus Spreading Center. Variations in alkali and dissolved gas abundances with Cl at PACMANUS and NE Pual suggest that phase separation has affected fluid chemistry despite the low temperatures of many vents. In further contrast to Vienna Woods, substantial modification of PACMANUS/NE Pual fluids has taken place as a result of seawater ingress into the upflow zone. Consistently high measured Mg concentrations as well as trends of increasingly non-conservative SO4 behavior, decreasing endmember Ca/Cl and Sr/Cl ratios with increased Mg indicate extensive subsurface anhydrite deposition is occurring as a result of subsurface seawater entrainment. Decreased pH and endmember Fe/Mn ratios in higher Mg fluids indicate that the associated mixing/cooling gives rise to sulfide deposition and secondary acidity production. Several low temperature (?80 °C) fluids at PACMANUS/NE Pual also show evidence for anhydrite dissolution and water-rock interaction (fixation of B) subsequent to seawater entrainment. Hence, the evolution of fluid compositions at Pual Ridge reflects the cumulative effects of water/rock interaction, admixing and reaction of fluids exsolved from silicic magma, phase separation/segregation and seawater ingress into upflow zones. 相似文献
160.
Andrew N. O’Brien Miroslav D. Filipović Evan J. Crawford Nicholas F. H. Tothill Jordan D. Collier Ain Y. De Horta Graeme F. Wong Danica Drašković Jeff L. Payne Thomas G. Pannuti Jared P. Napier Stephen A. Griffith Wayne D. Staggs Srdjan Kotuš 《Astrophysics and Space Science》2013,347(1):159-168
A series of new radio-continuum (λ=20, 13, 6 and 3 cm) mosaic images focused on the NGC 55 galactic system were produced using archived observational data from the Australia Telescope Compact Array. These new images are both very sensitive (down to rms=33 μJy) and feature high angular resolution (down to <4″). Using these newly created images, 66 previously unidentified discrete sources are identified. Of these sources, 46 were classified as background sources, 11 as H?ii regions and 6 as supernova remnant candidates. This relatively low number of SNR candidates detected coupled with the low number of large H?ii regions is consistent with the estimated low star formation rate of the galaxy at 0.06 M ⊙?year?1. Our spectral index map shows that the core of galaxy appears to have a shallow spectral index between α=?0.2 and ?0.4. This indicates that the core of the galaxy is a region of high thermal radiation output. 相似文献