A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01

Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3’-phosphoadenosine 5’-phosphosulfate (PAPS) to N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2 , Mg2 , Mn2 and Ca2 individually, but is inhibited by Cu2 . Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.     

New Damage-locating Method for Bridges Subjected to a Moving Load

A new damage-locating method for bridges subjected to a moving load is presented, and a new ‘moving load damage-locating indicator＇ （MLDI） is introduced. A vehicle is modeled as a moving load, the bridge is simplified as an Euler-Bernoulli beam, and the damage is simulated by a reduction of stiffness properties of the elements. The curvature and MLDI values at each node of the baseline model （undamaged） and the damage model are computed respectively. Then the damage or damages can be located from a sudden change of the MLDI value. The feasibility and effectiveness of the proposed method are validated by numerical simulation. The results indicate that the method is effective, being able to not only locate a single damage accurately, but also locate multiple damages in simply-supported bridges, including multiple damages in continuous bridges. The results also indicate that the MLDI can accurately locate damages under 5% measurement noise.     

A modified Hoek-Brown failure criterion considering the damage to reservoir bank slope rocks under water saturation-dehydration circulation

After water is impounded in a reservoir, rock mass in the hydro-fluctuation belt of the reservoir bank slope is subject to water saturationdehydration circulation (WSDC). To quantify the rate of change of rock mechanical properties, samples from the Longtan dam area were measured with uniaxial compression tests after different numbers (1, 5, 10, 15, and 20) of simulated WSDC cycles. Based on the curves derived from these tests, a modified Hoek- Brown failure criterion was proposed, in which a new parameter was introduced to model the cumulative damage to rocks after WSDC. A case of an engineering application was analyzed, and the results showed that the modified Hoek-Brown failure criterion is useful. Under similar WSDC-influenced engineering and geological conditions, rock mass strength parameters required for analysis and evaluation of rock slope stability can be estimated according to this modified Hoek-Brown failure criterion.     

Spatial structural pattern and vulnerability of China-Japan-Korea shipping network

The economies of China-Japan-Korea(CJK) are complementary, with their proximity resulting in the three countries having a high degree of interdependence with respect to trade. Currently, trade among these countries relies mainly on port-centered shipping. The development of the shipping network is integral for in-depth integration of CJK trade. This paper analyzes the overall characteristics, centrality, spatial structure, and vulnerability of the CJK shipping network using the methods of complex network analysis, blocking flow theory, and interruption and deletion of hub ports. The main findings are as follows: 1) The CJK shipping network has a small average path length and clustering coefficient, and its degree distribution follows a power-law distribution, which make the network present obvious characteristics of a Barabási-Albert scale-free. 2) The characteristics of the multi-center point of the CJK shipping network can alleviate traffic pressure. At the same time, the network shows a clear hierarchy in the port transportation system, with cargo transport relying mainly on the ‘hub port-hub port' connection. 3) The CJK shipping network is relatively stable. Compared with ports in Japan and Korea, the main hub ports in China have a greater impact on the stability of the shipping network, in particular those ports of the central coastal region, including Shanghai, Ningbo, and Lianyungang.     

Screening white spot syndrome virus (WSSV)-resistant molecular markers from <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis>

White spot syndrome virus (WSSV)-resistant molecular markers were screened from the selectively bred new variety ‘Huanghai No. 2’ of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting (HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and -resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms (SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89AT, genotype A/A of C2635-527CA and genotype C/T of contig C12355-592CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145AG and genotype C/C of contig C12355-592CT, were negatively correlated. Moreover, analysis with BlastX program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis.     

Combined adaptive multiple subtraction based on optimized event tracing and extended wiener filtering

The surface-related multiple elimination (SRME) method is based on feedback formulation and has become one of the most preferred multiple suppression methods used. However, some differences are apparent between the predicted multiples and those in the source seismic records, which may result in conventional adaptive multiple subtraction methods being barely able to effectively suppress multiples in actual production. This paper introduces a combined adaptive multiple attenuation method based on the optimized event tracing technique and extended Wiener filtering. The method firstly uses multiple records predicted by SRME to generate a multiple velocity spectrum, then separates the original record to an approximate primary record and an approximate multiple record by applying the optimized event tracing method and short-time window FK filtering method. After applying the extended Wiener filtering method, residual multiples in the approximate primary record can then be eliminated and the damaged primary can be restored from the approximate multiple record. This method combines the advantages of multiple elimination based on the optimized event tracing method and the extended Wiener filtering technique. It is an ideal method for suppressing typical hyperbolic and other types of multiples, with the advantage of minimizing damage of the primary. Synthetic and field data tests show that this method produces better multiple elimination results than the traditional multi-channel Wiener filter method and is more suitable for multiple elimination in complicated geological areas.     

Effects of varying densities on serum reproductive parameters in pen-reared juvenile female rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis> farms

The primary goal of this study was to assess the effect of varying densities on serum reproductive parameters of immature rainbow trout Oncorhynchus mykiss. Experimental trout were maintained in intensive, pen-reared farms for 300 days in fresh water reservoirs. Initial densities were 4.6, 6.6, and 8.6 kg/m³ (40, 60, 80 ind./m³), indicated as SD1, SD2, SD3, and final densities were 31.1, 40.6, 49.3 kg/m³, respectively. A summary of the ovarian stages were observed by histological examination. Serum E₂ (estradiol), T (testosterone) were evaluated by radioimmunoassay and FSH (follicle-stimulating-hormone), LH (luteinizing-hormone), vitellogenin, 17α,20β-P (17α,20βdihydroxy4-pregnen-3-one) were measured by enzyme-linked immunosorbent assay. Our findings demonstrated that ovarian development were retarded (from stage III to stage IV) at highest rearing density (SD3) after 180 days of intensive culture (over 40.6 kg/m³). In addition, we observed an inverse relationship between serum reproductive parameters and rearing density. Furthermore, compared to serum reproductive parameters of SD1, E₂, T, FSH, vitellogenin, 17α,20β-P, GSI and LH of two higher density groups decreased firstly and significantly at 60 (over 15.9 kg/m 3 ), 180 (over 31.7 kg/m 3 ), 180 (over 40.6 kg/m³), 240 (over 36 kg/m³), 240 (over 36 kg/m³), 240 (over 45 kg/m³) and 300 (over 49.3 kg/m³) days, respectively. Comparing serum reproductive parameters within the same ovarian development stage of rainbow trout from varying densities revealed that higher population density also led to significantly lower overall serum reproductive parameters. Overall, this study presents the reproductive, endocrinological parameters of juvenile female rainbow trout at high rearing densities and indicates the need for rainbow trout (114.44±5.21 g, 19.69±0.31 cm) that are initially stocked at 6.6 or 8.6 kg/m³ should be classified and subdivided into lower density after 180 days of farming (not over 31.7 kg/m³).     

Virtual Field Geologic Trip System

Virtual Field Geologic Trip System (VFGTS) constructed by the technique of visualization can efficiently present geologic field information and widely used in the field of geologic education. This paper introduces the developing thinking of VFGTS and discusses the main implement processes. Building VFGTS mainly includes systemically gathering of field geological data, the building of virtual geological world, and displaying of virtual geologic world and human-computer interaction.     

SIMULATION AND PREDICTION OF DEBRIS FLOW USING ARTIFICIAL NEURAL NETWORK

Debris flow is one of the most destructive phenomena of natural hazards. Recently, major natural haz-ard, claiming human lives and assets, is due to debris flow in the world. Several practical methods for forecasting de-bris flow have been proposed, however, the accuracy of these methods is not high enough for practical use because of the stochastic and non-linear characteristics of debris flow. Artificial neural network has proven to be feasible and use-fill in developing models for nonlinear systems. On the other hand, predicting the future behavior based on a time se-ries of collected historical data is also an important tool in many scientific applications. In this study we present a three-layer feed-forward neural network model to forecast surge of debris flow according to the time series data collect-ed in the Jiangjia Ravine, situated in north part of Yunnan Province of China. The simulation and prediction of debris flow using the proposed approach shows this model is feasible, however, further studies are needed.     

青藏高原东北缘断层活动变形的模拟研究

分析和讨论了位错理论模型的发展历史和应用领域，采用走滑和倾滑位错理论模型对青藏高原东北缘的多组断层活动产生的三维形变场进行了数值模拟研究，并将数值模拟结果与该地区1999～2001年间的GPS观测结果进行了对比分析。得出了甘青块体顺时针转动等一些较重要的结论和认识。