近50年青藏高原东部降雪的时空演变

选用1967-2012年青藏高原东部60个站点的观测资料,分析了该地区降雪的时空演变特征,并结合降水和气温的变化,探讨了降雪与积雪的关系,结果表明：青藏高原东部年降雪量在1.3~152.5 mm范围内变化,空间分布差异显著;秋季降雪表现出中间多、周边少的特征,冬季降雪表现出由东南向西北递减的特征,春季降雪最多且空间分布与年降雪基本一致;降雪可划分为青南高原区、藏北高原区、柴达木盆地区、青藏高原东南缘区、川西高原西北部区、青藏高原南缘区、青海东北部区及藏南谷地区;就青藏高原整体而言,除秋季外,整年、冬季和春季降雪均表现出“少—多—少”的年代际变化特征,其中冬季降雪在1986年发生了由少到多的突变,整年、冬季和春季降雪均在1997年发生了由多到少的突变;不同区域降雪的时间变化规律各具特点;降雪与积雪的关系十分密切,春季降雪受气温的影响最为显著,秋季次之,冬季最弱;20世纪末,春季降雪受气温升高的影响表现出与降水变化相反的由多到少的气候突变特征。     

动态分段思想在MapInfo中的实现

动态分段是一种新的线性特征的动态分析、显示和绘图技术.目前该技术已经成功地应用在以Arc/Info为代表的采用"弧段-结点"数据模型的GIS软件中,但是在采用"面向实体"数据模型的GIS软件中实现动态分段的研究却很少.本文在分析Arc/Info中动态分段数据结构的基础上,探讨如何在MapInfo中实现动态分段思想.     

Impacts of land use changes on groundwater resources in the Heihe River Basin

Land use and land cover changes have a great impact on the regional hydrological process. Based on three periods of remote sensing data from the 1960s and the long-term observed data of groundwater from the 1980s, the impacts of land use changes on the groundwater system in the middle reach of Heihe River Basin in recent three decades are analyzed by the perspective of groundwater recharge and discharge system. The results indicate that with the different intensities of land use changes, the impacts on the groundwater recharge were 2.602×108 m3/a in the former 15 years (1969-1985) and 0.218×108 m3/a in the latter 15 years (1986-2000), and the impacts on the groundwater discharge were 2.035×108 m3/a and 4.91×108 m3/a respectively. When the groundwater exploitation was in a reasonable range less than 3.0×108 m3/a, the land use changes could control the changes of regional groundwater resources. Influenced by the land use changes and the large-scale exploitation in the recent decade, the groundwater resources present apparently regional differences in Zhangye region. Realizing the impact of land use changes on groundwater system and the characteristics of spatial-temporal variations of regional groundwater resources would be very important for reasonably utilizing and managing water and soil resources.     

贵州红枫湖纹理沉积物中近代气温记录

本通过红枫湖纹理沉积物柱芯碳酸盐特征剖面与流域气温资料对比、沉积纹理年代校正以及早期成岩化学过程对碳酸盐沉积记录的改造作用等方面的研究，表明湖泊碳酸盐沉积记录基本不受早期成岩作用的影响，碳酸钙含量和ＣａＯ／ＭｇＯ·Ａｌ２Ｏ３比值可作为湖泊沉物中具高分辨率、短时间尺度的气温变化的代用指标。     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

沙拐枣属（Calligonum L.）7种植物总DNA提取方法的改进及鉴定

 摸索沙拐枣属（Calligonum L.）七种植物沙拐枣（Calligonum mongolicum Turcz. ）、泡果沙拐枣（C. junceum (Fisch. et Mey.) Endl.）、无叶沙拐枣（C. aphyllum (Pall.) Gürke）、红果沙拐枣（C. rubicundum Bge.）、心形沙拐枣（C. cordatum E. Kor. ex N. Pavl.）、密刺沙拐枣(C. densum Borszcz.)、乔木状沙拐枣（C. arborescens Litv.）的总DNA提取方法并对DNA样品的纯度和浓度进行鉴定,对传统的CTAB法进行方法和材料的改进,开展与总DNA相关的PCR扩增和其他遗传学分析,并取得满意的结果。其结果对于沙拐枣属植物的有关研究提供了有效的实验手段。     

基于DGT和化学提取法研究浙西北地质高背景区土壤镉生物有效性

地质高背景区相较于人类活动引起的土壤镉污染影响范围更广,在区域尺度上对生态系统和人类健康构成危害。土壤镉生物有效性是决定其生物可利用性、生物毒性的关键因素,因此探寻可行的土壤镉生物有效性评价方法对污染农用地安全利用和风险管控具有重要的理论和实际意义。DGT技术、单一提取法、连续提取法和土壤溶液法常用于测定土壤有效镉,但已有研究成果主要基于同种土地利用类型土壤的室内盆栽实验,难以代表自然污染土壤中的复杂情况。为探明各土壤重金属有效态提取技术对地质高背景区不同土地利用类型土壤Cd生物有效性评估效果,本文以浙江西北部土壤Cd高地质背景区水田土壤-水稻籽实和旱地土壤-小白菜样品为研究对象,实验应用DGT技术、单一提取法（0.01mol/L氯化钙提取）、连续提取法（七步连续提取）和土壤溶液法评价土壤中镉生物有效性。结果显示：①研究区水田和旱地土壤Cd平均含量分别为1.07mg/kg和0.73mg/kg,显著高于浙江和全国土壤平均水平,Cd的异常富集主要与浙西北地区广泛分布的黑色岩系有关。②相较于碳酸盐岩区,黑色岩系区土壤中Cd的生物有效组分占比较高,水田和旱地土壤Cd的活动系数（MF）高达59.9%和51.8%,Cd易在土壤-作物系统中发生迁移富集;③植物体内镉含量Cd-P与不同方法测定的有效镉含量均呈显著正相关,但Cd-P与DGT技术测定的有效镉含量相关性优于其他三种方法,水田土壤测得的有效Cd与水稻籽实相关关系：$ {{C}}_{\text{soln}} $>CDGT>$ {{C}}_{{\text{CaCl}}_{\text{2}}} $>$ {{C}}_{{\text{F}}_{\text{1}}\text{+}{\text{F}}_{\text{2}}\text{+}{\text{F}}_{\text{3}}} $,旱地土壤测得的有效Cd与小白菜相关关系：CDGT >$ {{C}}_{{\text{CaCl}}_{\text{2}}} $>$ {{C}}_{{\text{F}}_{\text{1}}\text{+}{\text{F}}_{\text{2}}\text{+}{\text{F}}_{\text{3}}} $>$ {{C}}_{\text{soln}} $。综合比较不同土壤有效Cd测定方法的优缺点,DGT技术可以模拟植物体对Cd的动态吸收过程,更能准确地反映土壤Cd生物有效性,预测作物Cd含量水平,这与已有研究成果一致。此外,本文研究成果表明DGT技术评价土壤Cd生物有效性,不仅适用于人为污染区,也可应用于地质高背景区。     

北京市生产性服务业的增长及其空间结构

基于北京1997年投入产出表、2002年投入产出表和第一次经济普查数据,采用投入产出模型、区位商等方法.分析北京市生产性服务业的增长结构变化、对地区经济的影响,以及典型生产性服务业行业的空间结构.研究表明.北京生产性服务业明显呈现出以金融、商务和信息服务业为主的格局;生产性服务业主要为第三产业服务;服务业对金融、商务、信息等生产性服务业的中间需求呈上升趋势;制造业对中间投入服务的消耗层次明显提高,产业结构不断优化升级;生产性服务业既有较强的经济推动能力,又对经济发展的需求较大;北京市生产性服务业的空间结构总体呈现圈层特征.     

An analytical study on three‐dimensional versus two‐dimensional water table‐induced flow patterns in a Tóthian basin

Although it has been increasingly acknowledged that groundwater flow pattern is complicated in the three‐dimensional (3‐D) domain, two‐dimensional (2‐D) water table‐induced flow models are still widely used to delineate basin‐scale groundwater circulation. However, the validity of 2‐D cross‐sectional flow field induced by water table has been seldom examined. Here, we derive the analytical solution of 3‐D water table‐induced hydraulic head in a Tóthian basin and then examine the validity of 2‐D cross‐sectional models by comparing the flow fields of selected cross sections calculated by the 2‐D cross‐sectional model with those by the 3‐D model, which represents the “true” cases. For cross sections in the recharge or discharge area of the 3‐D basin, even if head difference is not significant, the 2‐D cross‐sectional models result in flow patterns absolutely different from the true ones. For the cross section following the principal direction of groundwater flow, although 2‐D cross‐sectional models would overestimate the penetrating depth of local flow systems and underestimate the recharge/discharge flux, the flow pattern from the cross‐sectional model is similar to the true one and could be close enough to the true one by adjusting the decay exponent and anisotropy ratio of permeability. Consequently, to determine whether a 2‐D cross‐sectional model is applicable, a comparison of hydraulic head difference between 2‐D and 3‐D solutions is not enough. Instead, the similarity of flow pattern should be considered to determine whether a cross‐sectional model is applicable. This study improves understanding of groundwater flow induced by more natural water table undulations in the 3‐D domain and the limitations of 2‐D models accounting for cross‐sectional water table undulation only.     

Test on dynamic characteristics of subgrade of heavy-haul railway in cold regions

Dynamic characteristics of heavy-haul railway subgrade under vibratory loading in cold regions are investigated via low-temperature dynamic triaxial tests with multi-stage cyclic loading process. The relationship between dynamic shear stress and dynamic shear strain of frozen soil of subgrade under train loading and the influence of freezing temperatures on dynamic constitutive relation, dynamic shear modulus and damping ratio are observed in this study. Test results show that the dynamic constitutive relations of the frozen soils with different freezing temperatures comply with the hyperbolic model, in which model parameters a and b decrease with increasing freezing temperature. The dynamic shear modulus of the frozen soils decreases with increasing dynamic shear strains initially, followed by a relatively smooth attenuation tendency, whereas increases with decreasing freezing temperatures. The damping ratios decrease with decreasing freezing temperatures. Two linear functions are defined to express the linear relationships between dynamic shear modulus (damping ratio) and freezing temperature, respectively, in which corresponding linear coefficients are obtained through multiple regression analysis of test data.