Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

渤海湾天津近岸海域水环境空间变异分析

由于海岸带的地理条件和海洋动力特性的复杂性以及人类开发活动对海岸带的影响,近岸海域的生态环境系统在空间分布上有很大的变异性.基于现场实测资料,建立系统的空间变异分析模型,对海岸带生态环境空间变化规律进行分析,可以充分了解不同区域的污染特征和变化趋势.应用所构建的空间变异分析模型对渤海湾天津近岸海域的水环境特性进行分析,结合对应年份的入海流量及其变化,分析了天津渤海湾海岸带污染的主因子和区域水环境的分布特征,结果可以对海洋功能区的划分和水环境综合评价结果进行验证和补充.     

软土海岸地区测量控制点标志的设立

分析了软土、湿地、滩涂地区特殊地理环境对测量控制点的影响;主要探讨了此类地区控制点标志的设计、施工。借助于建筑桩基技术和道桥施工方法,首次成功实现了某三角洲湿地滩涂地区统一布网。几年来,结合滩涂湿地测量、海岸防护和滩涂开发工程对部分控制点进行了初步检测,给出了部分检测数据。应用和检测均表明:桩基标石可较好地解决软土、湿地、滩涂地区控制点布设难题,显著提高软土海岸地区测量控制点标志的稳固性,从而延长控制网的可使用期。     

渤海生态动力过程的模型研究 Ⅱ.营养盐以及叶绿素a的季节变化

利用在本系列研究第一部分中所建立的耦合的生物物理模型,模拟了渤海浮游植物生物量和营养盐含量的年度循环特征.模拟结果显示:藻类的春季水华是由经过一冬积累在水体中的营养盐导致,而水华开始的时间在浅水区明显早于深水区,对此深水区水体层化结构的形成可能起着重要作用;另一方面,河载营养盐与悬起的沉积物所释放的营养盐是诱发夏季水华的共同原因.基于模型结果,我们还发现:渤海的浮游植物动力特性就整体而言依然受无机氮限制,但是在莱州湾,磷限制特性表现得非常明显,这主要是由于每年黄河都要携带大量的无机氮进入海水,从而导致莱州湾营养盐的氮磷比已远远超过16.     

我国近海沉积层地震速度特征

我国近海纵向上发育了新生界、中生界碎屑岩和古生界沉积地层。在分析整理收集到的地层速度资料的基础上,分析了地层速度的变化规律:沉积层地震速度随地层的埋深增加而增加,新地层速度低、老地层速度高;欠压实地层的砂岩速度与泥岩速度基本相当或低于泥岩速度,正常地层的砂岩速度大于泥岩速度;砂岩储层的速度随孔隙度的增加而降低,流体成分的变化对速度的影响不大。     

南极冰藻类囊体膜光系统研究进展

南极冰藻是南大洋碳流和能量流的重要组成部分,在该区域的生态系统中发挥着极其重要的作用.低温、低光照和高盐度对类囊体膜光系统的伤害必将影响冰藻对光能的吸收、传递和转换,从而影响冰藻的正常生长繁殖.事实上,冰藻为了能够在这寒冷骤变的环境中生存、繁衍,类囊体膜光系统在生理、代谢和遗传上进行了复杂的适应性改变,冰藻在低光照条件下保持很高的光合效率.因此研究南极冰藻类囊体膜光系统具有重要的理论意义.综述了南极冰藻类囊体膜光系统的研究进展,以期为进一步了解南极冰藻光系统作用机理提供依据.     

1993—2001年全球海面高度变化特征

应用TOPEX／POSEIDON（T／P）卫星高度计测高资料，对全球海洋的海面变化特征进行了分析，结果表明，1993年1月－2001年6月期间，全球海平面呈现上升的态势；全球平均海平面高度的平均上升速率约为1．2mm/a；海温的变化是引起海平面变化的重要原因，便其对海平面抬升的贡献不到50％。海平面的变化具有很强的地域特征。海平面变化的空间分布特征受风应力异常特别是纬向风应力异常的空间分布影响较大。     

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.     

The Structure of A Turbulent Jet in A Crossflow-Effect of Jet-Crossflow Velocity

A comprehensive numerical study on the three-dimensional structure of a turbulent jet in crossflow is performed. The jet-to-crossflow velocity ratio (R) varies in the range of 2 - 16; both vertical jets and inclined jets without excess streamwise momentum are considered. The numerical results of the Standard two-equation k-ε model show that the turbulent structure can be broadly categorised according to the jet-to-crossflow velocity ratio. For strong to moderate jet discharges, i.e. R> 4, the jet is characterized by a longitudinal transition through a bent-over phase during which the jet becomes almost parallel with the main freestream, to a sectional vortex-pair flow with double concentration maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless distance of around 20-60. The similarity coefficients are only weakly dependent on R. The cross-section scalar field is kidney-shaped and bifurcated, vvith distinct double concentr     

赤潮叉角藻18SrDNA和ITS区序列测定与分析

采用PCR及克隆测序的方法，对1998年引发渤海赤潮的叉角藻18SrRNAadldey DNAITS区（Internal Transcribed Spacer Regions)进行了序列测定与分析。并通过因特网从国际分子生物学数据库中获取甲藻另外15个种的18rDNA序列，以Tetrahymena corlissi作为类群，分别采用Neighbor-Joining和Fitch方法构建甲藻较为一致和可靠的进化树图，探讨具有高度多样性和在分类上争议较多的甲藻各类群之间的形态与分子进化关系。结果表明，Prorocentrum(有2个简单的壳板）出现得较早，而大多数多甲藻目（覆盖着多个壳板）、裸甲藻目（大多数不具壳板）和膝沟藻目的成员较晚出现。另外，对叉角藻ITS区的分析表明，ITS区为高变区，是良好的分子标记，可用于叉角藻快速鉴定的专一性核酸分子探针的研制。