4种酞酸酯在龙须菜-篮子鱼食物链中的积累放大研究

在模拟受控条件下,利用气相色谱方法研究分析了4种酞酸酯类化合物在龙须菜(Gracilaria lemane-iformis)-篮子鱼(Siganus oramin)食物链中的积累、分布和放大状况。结果表明,4种酞酸酯在龙须菜组织内的积累表现为:邻苯二甲酸二异辛酯(DEHP)>邻苯二甲酸二丁酯(DBP)>邻苯二甲酸二乙酯(DEP)>邻苯二甲酸二甲酯(DMP)。侧链最长的DEHP在龙须菜藻体内的含量随暴露时间的延长呈增加的趋势,30 d体内积累量可达2.35 mg/kg(鲜质量);侧链最短的DMP在龙须菜藻体内的质量比随时间变化差异不显著,约为0.05 mg/kg;在30 d的暴露中,4种酞酸酯DMP,DEP,DBP和DEHP在篮子鱼体内总质量比分别为0.08,0.41,1.72,3.11 mg/kg。其中DBP和DEHP在鱼内脏组织中有较高的积累和分布,分别为1.14 mg/kg和1.83 mg/kg,其次为鱼残体组织,肌肉组织中含量最低。DEHP和DBP在龙须菜和篮子鱼体不同组织中均有一定程度的富集(生物富集系数FBC均大于1)。其中DEHP和DBP在内脏组织中的FBC值分别高达9.16和5.68,在龙须菜-篮子鱼这个简单食物链传递中DEHP和DBP在篮子鱼内脏组织中存在放大现象。     

基于WebGIS长江流域防洪调度系统的研究与实现

从长江流域防洪调度实际工作出发,针对长江流域防洪指挥特点,利用ArcGIS Server,ArcSDE与.Net平台相结合,进行了B/S模式的长江流域防洪调度WebGIS系统设计与实现。系统利用高效、便捷的Internet/Intra-net网络资源和基于GIS技术的地图操作功能,实现了长江流域防汛指挥的全局化、实时化、可视化。     

Resolving physically and biologically driven suspended particulate matter dynamics in a tidal basin with a distribution-based model

Understanding suspended particulate matter (SPM) dynamics in coastal waters is crucial to assess changes in coastal sediment budgets and biogeochemical fluxes. SPM dynamics are subject to various physical and biological factors and processes such as, e.g. tidal currents and aggregation which can be enhanced by extracellular polymeric substances (EPS) that are produced by algae and bacteria. It is still unclear how the different factors and processes interact and together determine SPM dynamics. To unravel the interacting processes and factors, we propose a new distribution-based modeling approach. Based on the derivation of explicit equations for size distribution characteristics of SPM such as the average radius, we derived a model of reduced complexity characterized by low initialization and parameterization effort as well as low computational cost. The proposed 0D model includes the processes of aggregation and fragmentation due to shear, aggregation due to differential settling, deposition, resuspension and tidal exchange, and describes the evolution of the SPM concentration in the water column linked by the settling velocity to the change of the mass average radius of the aggregate distribution. A systematic parameter variation for critical bottom shear stress of erosion, the size of resuspended aggregates, the fractal dimension, the collision efficiency, and the aggregate strength has been performed and compared to observations in the back-barrier basin of Spiekeroog Island in the German Wadden Sea. This analysis confirms the hypothesis that in winter biological influences on SPM dynamics are smaller compared to summer. This is mainly reflected by a significant shift in the various parameters. We hence conclude that biological control mechanisms have a much more quantitative relevance for SPM dynamics than currently represented by state-of-the-art SPM transport models.     

基于IMU旋转的船用激光导航系统分析与设计

利用惯性测量单元(IMU)旋转技术可以有效调制惯性元件的零位偏置,提高惯性导航系统的长时间导航精度.针对船用惯性导航系统的应用需求,设计了IMU单轴旋转的船用激光陀螺捷联式惯性导航系统.分析了IMU单轴旋转的工作机理,并通过仿真进行了验证.对导航系统的总体方案、原理方案和旋转方案的原理和实现进行了论述,初步的性能试验验证了设计方案的有效性.     

Approach to modeling and virtual-reality-based simulation for plant canopy lighting

Over the past 20 years, significant progress has been made in virtual plant modeling corresponding to the rapid advances in information technology. Virtual plant research has broad applications in agronomy, forestry, ecology and remote sensing. As many biological processes are driven by light, it is the key for virtual plant to estimate the light absorbed by each organ. This paper presents the radiance equation suitable for calculating sun and sky light intercepted by plant organs based on the principles of the interaction between light and plant canopy firstly; analyzes the process principles of plant canopy primary lighting based on ray casting and projection secondly; describes the multiple scattering of plant lighting based on Monte Carlo ray tracing method and on the radiosity method thirdly; and confirms the research with 3D visualization based on Virtual Reality Modeling Language （VRML） finally. The research is the primary work of digital agriculture, and important for monitoring and estimating corn growth in Northeast China.     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

不同倍性大黄鱼核仁数目银染观察

用银染法对不同倍性(单倍体、正常二倍体、正常三倍体、雌核发育二倍体、雌核发育鱼子代三倍体)大黄鱼胚胎、仔鱼或成鱼的不同组织进行细胞核仁数目观察计数。结果表明,大黄鱼各种倍性个体细胞核仁数目与染色体倍性之间有良好的对应关系,且核仁数随个体倍性的增加而增加;非诱导雌核发育的大黄鱼细胞核仁众数与染色体组数相等(二倍体为2,三倍体为3),而人工诱导雌核发育大黄鱼及其子代细胞核仁众数却比其相同倍性的非雌核发育鱼少1(雌核发育二倍体为1,雌核发育三倍体为2)。雌核发育鱼核仁数减少是一个非常有趣的现象,值得进一步研究。大黄鱼不同发育阶段(胚胎、仔鱼、成鱼)以及同一个体不同组织(鳃、肾、鳍)的细胞核仁数目(众数)相同。因此,借助核仁银染法可以快速、可靠、简便地进行染色体倍性鉴定。而雌核发育鱼核仁数减少的机理值得进一步研究。     

极地产适冷脂肪酶菌株的酶学性质研究

从南北极环境样品中分离到7株产脂肪酶的细菌,经16SrDNA序列分析表明,这些细菌分别属于假交替单胞菌属（Pseudoalteromonas）和嗜冷杆菌属（Psychrobacter）．采用p-NPP法对这7株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在30～40cC、最适作用pH值在7—8之间,在低温下能保持较高的剩余活力,对热敏感,属于适冷脂肪酶．其中假交替单胞菌（Psychrobacter sp．7342）所产脂肪酶具有低温下酶的剩余活力高、有效作用温度和pH范围广、热稳定性较好及对多种金属离子抗性强等特点．该菌株能利用多种单一氮源和碳源产酶,最适产酶温度为25℃．在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为：蛋白胨和淀粉含量各为1．33％,酵母膏含量为0．3％,温度为25℃．     

北黄海潮流、余流垂直结构及其季节变化

基于对2006年夏季与2007年冬季在123.51°E,38.00°N的各1个月潮流和水位观测数据的分析,发现夏季余流呈现两层结构,上层流向为西北,下层流向东南,并且当大潮日期附近,在跃层附近深度存在若干流速较强的水层;冬季余流除了个别层以外基本上均为西北向流入北黄海,从中可以看到风场改变所引发的异常增减水和强流出现。对潮流椭圆的分析表明,半日潮族分潮流的最大流向自夏季至冬季存在着顺时针的旋转,旋转角大约为16(°)～18(°),并且夏季半日潮族随深度顺时针,全日潮族逆时针旋转,而冬季基本上上下一致。     

闽中近岸西北行路径台风浪波向特征

台风浪的研究对于船舶航行、避风以及港口、海洋和近岸建筑物的安全有着重要的现实意义。本文基于考虑波浪折射、底部损耗及波浪破碎等的波谱模型,在充分考虑风能量输入、白帽耗散、波浪破碎以及波-波间的非线性相互作用等物理过程后,对在福建省中部登陆的0513号"泰利"、0709号"圣帕"和0808号"凤凰"台风引起的台风浪过程进行了数值模拟计算。计算结果与实测数据相吻合,并总结出闽中近岸西北行路径台风浪的波向变化规律。为近岸海域船舶航行和港口工程防灾减灾提供了较为重要的参考依据。