Different thermal preferences for brooding and larval dispersal of two neighboring shrimps in deep‐sea hydrothermal vent fields

Temperature is an important factor affecting the distributions and life‐history traits of marine animals. Deep‐sea hydrothermal vents are suitable environments to examine ecologic differences related to temperature, due to the steep temperature gradients around the vents. Rearing experiments under various temperature conditions (5–30 °C) at atmospheric pressure demonstrated a difference in thermal effects on egg hatching and larva in two co‐occurring, vent‐associated alvinocaridid shrimps – the peripherally distributed Alvinocaris longirostris and the centrally distributed Shinkaicaris leurokolos. The duration before hatching became shorter as temperature increased, while the maximum hatching rate occurred at higher temperatures in S. leurokolos (10–20 °C) than in A. longirostris (10 °C). Hatched larvae of both species were negatively buoyant, and larva with normal abdominal length could actively swim and stay suspended in the mid‐ or surface water layers of the culture plates under our experimental conditions. However, no larvae settled or metamorphosed into juveniles under the rearing conditions used in this study. Larvae with shortened abdomens occurred under most of the experimental conditions, although they were less frequent at 10 °C in A. longirostris and 20 °C in S. leurokolos. The maximum survival periods at these temperatures were 88 days in A. longirostris and 30 days in S. leurokolos. These characteristics may cause differences in the distributional ranges of the two species. The present results indicate that temperature is an important factor controlling life‐history traits of vent shrimps.     

日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

2014年春季季风间期东印度洋赤道及其邻近海域硅藻群落

2014年4月10日至5月13日在东印度洋赤道区及其邻近海域(10.08°N-6.00°S,80.00°~96.10°E)进行硅藻物种组成和群落结构的调查。分析了45个网采样品,共鉴定出浮游硅藻34属113种(包括变种、变形及未定名种),大部分物种为热带外洋性种以及暖海外洋性种,与该海区的热带及亚热带的环境特征一致。优势种为佛氏梯形藻(Climacodium frauenfeldianum)、地中海细柱藻(Leptocylindrus mediterraneus)、密聚角毛藻(Chaetoceros coarctatus)、美丽漂流藻(Planktoniella foromsa)、大西洋角毛藻那不勒斯变种(Chaetoceros atlanticus var. neapolitanus)、距端假管藻(Pseudosolenia calcar-avis,即距端根管藻Rhizosolenia calcaravis)、圆柱几内亚藻(Guinardia cylindrus)、达蒂角毛藻(Chaetoceros dadayi)、伏氏海线藻(Thalassionema frauenfeldii)、离心列海链藻(Thalassiosira excentrica)、瘤面角毛藻(Chaetoceros bacteriastroides)以及笔尖根管藻粗径变种(Rhizosolenia styliformis var. latissima)等。硅藻的平均细胞丰度为1.855×103个/m3,其平面分布不均匀,赤道断面细胞丰度较低,高值区出现在海区北部。聚类分析发现有7种生态类群,这些硅藻的种类和丰度平面分布与上层水体(200 m以浅)温度、盐度及营养盐的水柱平均值有较好的对应关系。     

莱州湾拟哲水蚤Paracalanus sp.发育期丰度组成和个体大小季节变化

拟哲水蚤是莱州湾桡足类中周年出现的优势种;是上层鱼类幼鱼早期开口饵料的重要贡献者;掌握其种群动态分布特点;有利于了解湾内生物环境状况、次级生产力生产水平。基于2011年5月至20 12年4月采集的浮游生物资料的分析结果显示;莱州湾拟哲水蚤的发育期丰度组成和个体大小均表现了明显的季节变化。调查季节中;4-6月以成体居多;其他季节以桡足幼体CⅢ-CⅤ较多;其中10-11月和3月主要由后期桡足幼体CⅣ和CⅤ期构成。成体中以雌体为主导;雌/雄比介于1.46~9.62;该比值在3-4月最低;10-11月最高。拟哲水蚤各期桡足幼体和成体个体大小以4-5月最大、8月最小;并与水温表现出明显的负相关。拟哲水蚤桡足幼体和成体的总生物量月变化与丰度变化相同;即以8月值最高;3-5月值最低;月均值为2.69 mg/m3（以碳计）;日生产力估算值为0.74 mg/（m3·d）（以碳计）。由上述结果;推测莱州湾拟哲水蚤的种群结构周年变化主要可分3个阶段;4-6月是莱州湾拟哲水蚤开始繁殖期;7-8月为种群数量快速增长期;9月之后为种群增长相对停滞期。     

夏季南海北部微型浮游动物群落

根据2014年8月至9月于珠江口至南海中部断面(18°00'~22°00'N,114°00'~116°00'E)的南海北部海域进行采样调查,并进行了微型浮游动物群落分析。共发现微型浮游动物142种,隶属于2门44属,其中砂壳纤毛虫28属78种,占所有发现物种数的54.93%;寡毛类纤毛虫14属59种,占所有发现物种数的41.55%。优势类群为:拟卡金斯急游虫(Strombidium paracalkinsi)、具沟急游虫(Strombidium sulcatum)、维尔伯特急游虫(Strombidium wilberti)和无节幼体(nauplii)。调查区微型浮游动物的丰度介于11.43~959.35 ind/L之间,平均值为264.99 ind/L。微型浮游动物垂直分布总体特点是密集区位于50 m水层,50 m水层之下丰度逐渐减少。表层微型浮游动物丰度高值区位于J5-I1站位之间。断面的香农-威纳指数范围在0.92~4.18之间,平均值为2.77;均匀度指数在0.63~1之间,平均值为0.87。应用典范对应分析(CCA)发现温度和盐度是影响微型浮游动物群落的重要因素。通过对连续追踪站位的调查发现,上层水体微型浮游动物群落丰度随着时间而发生一定的变化,下层水体相对较平缓。微型浮游动物昼夜的垂直丰度变化与叶绿素浓度昼夜变化大致相符。     

2001年秋季风对坦帕湾盐度平衡的影响

利用三维高分辨率有限体积的近海海域模型FVCOM来分析2001年秋季期间风作用对坦帕湾区域盐度平衡的影响。为了区分风的影响,分别设计了两个实验：一个由潮汐和河流作为驱动,另一个由潮汐、河流以及风场共同驱动。结论如下：首先,风作用会使盐度产生变化,能够明显地使坦帕湾内的盐度增加,并导致水平和垂直方向上盐度梯度的减少;随后,本文分析了坦帕湾区域内的盐度平衡,主要的盐度平衡来自于全部（水平和垂直方向上）平流的盐度流量分歧以及除去海峡底部的垂直方向上盐度流量分歧;最后,对由风引起的盐度变化进一步进行分析,结果表明风作用不能改变盐度平衡地位的相对重要性,由风引起的盐度平衡改变高度依靠于特殊的地形,除此之外,全部平流盐度流量分歧和垂直散布盐度通量分歧能够抵消,并且两者都远大于水平散步盐度流量分歧。     

末次冰盛期后黄河三角洲沉积物的磁性特征及其沉积环境

On the basis of measuring the magnetic parameters of sediment in Core YDZ1, combined with a grain size analysis and Carbon-14 dating, the magnetic properties of sediment and sedimentary environment in the Huanghe(Yellow River) Delta area after the last glacial maximum have been studied. The results show that the ferrimagnetic minerals of a pseudo single domain and multi domain particles dominate the magnetic properties of sediment in Core YDZ1. The imperfect anti ferrimagnetic minerals have more contribution on sediment in a depth of 24.0–22.1 m, and more stable-single domain and pseudo single domain particles exist. The susceptibility of anhysteretic remanent magnetization and the ratio of the susceptibility of anhysteretic remanent magnetization to saturation isothermal remanent magnetization show a decrease trend below depth of 24 m, a marked increase trend in a depth of 24.0–13.5 m, and a rapid decrease at depth of 13.5 m, then a fluctuation trend upward. The above two magnetic parameters and the ratio of the susceptibility of anhysteretic remanent magnetization to the mass susceptibility can be regarded as the proxy indicators for the content of clay(4 μm)and the fine-grained size(32 μm). The sedimentary environment after the last glacial maximum in the Huanghe Delta area has experienced the fluvial facies, the tidal flat facies, the neritic facies, the pro delta facies, the delta front facies and the floodplain facies. Thickness of the Holocene transgression layer is 10.5 m and the depth of substrate is about 24 m according to the YDZ1 core. The sedimentary dynamic has a variation trend with strongweak-strong, which has been proved by the Flemming triangular schema.     

基于面向对象影像分析方法的我国西沙珊瑚礁地貌单元信息提取

珊瑚礁地貌单元的空间分布对于理解珊瑚礁生态系统的地质构造过程具有重要作用。然而,基于像素的影像分析方法往往获取不到较高精度的分类结果。本文基于面向对象的影像分析方法,利用Landsat 8卫星影像数据对我国西沙地区的珊瑚环礁进行了地貌单元的遥感信息提取。借鉴于美国千年珊瑚礁测绘项目的工作成果,本文首先针对研究区特点定义了十类珊瑚礁地貌单元类型。然后,基于对象的多层次关系特点,并综合利用对象的光谱、形状、上下文关系等特征,建立合适的分类规则集,获取了研究区较大尺度的珊瑚环礁地貌分区图,其分类精度普遍高于80%。虽然研究结果表明基于面向对象的影像分析方法可以有效的进行珊瑚礁遥感信息提取,但其规则集的可移植性仍需要今后的工作加以改善。     

Solar-, monsoon- and Kuroshio-influenced thermocline depth and sea surface salinity in the southern Okinawa Trough during the past 17,300 years

Factors influencing millennial-scale variability in the thermocline depth (vertical mixing) and sea surface salinity (SSS) of the southern Okinawa Trough (OT) during the past 17,300 years were investigated based on foraminifer oxygen isotope records of the surface dweller Globigerinoides ruber sensu stricto and the thermocline dweller Pulleniatina obliquiloculata in the AMS ¹⁴C dated OKT-3 core. The thermocline depth is influenced by surface thermal buoyancy (heat) flux, in turn controlled by the annual mean insolation at 30°N and the strength of the East Asian winter monsoon (EAWM). Strong insolation and weak EAWM tend to increase buoyancy gain (decrease buoyancy loss), corresponding to shallow thermocline depths, and vice versa. Regional SSS is influenced by the global ice volume, the Kuroshio Current (KC), and vertical mixing. A deep thermocline coincides with a high SSS because strong vertical mixing brings more, saltier subsurface KC water to the surface, and vice versa. Local SSS (excluding the global ice volume effect) became lower in the northern OT than in the southern OT after ~9.2 ka, implying that Changjiang diluted water had stronger influence in the northern sector. SSS show no major changes during the Bølling/Allerød and Younger Dryas events, probably because the KC disturbed the North Atlantic signals. This argues against earlier interpretations of sea surface temperature records of this core. Wavelet and spectral analyses of the Δδ¹⁸O_P-G (δ¹⁸O of P. obliquiloculata minus G. ruber s.s.) and δ¹⁸O_local records display 1,540-, 1,480-, 1,050-, 860-, 640-, and 630-year periods. These are consistent with published evidence of a pervasive periodicity of 1,500 years in global climate as well as EAWM and KC signatures, and a fundamental solar periodicity of 1,000 years and intermediary derived periodicity of 700 years.