基于兰利法的泥石流临界雨量实验研究

分析了目前泥石流临界雨量确定常用方法存在的问题 ,以及泥石流临界雨量实验研究中实验降雨量确定的随意性和盲目性 ,提出以兰利 ( Langlie)法为指导进行实验 ,使实验降雨量的确定有了理论依据 ,减少了取得可靠实验结果所需实验次数 ,从而使泥石流临界雨量实验研究更趋完善和可行     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

无实测资料河流逐月水量估算方法适用性研究

无实测资料河流逐月水量估算是最严格水资源管理制度考核的重要支撑。基于流域水文相似性原理和水量平衡原理,选用径流模数法、径流系数法、区域水量平衡法,研究提出其逐月水量估算适用范围和估算成果合理性分析标准。以现有水文站为假设断面,采用参证水文站资料进行假设断面水量估算,对三种方法的适用范围和估算成果合理性分析标准进行验证。结果表明,径流模数法适用于集水面积相差不超过20%情形和考虑区间蓄泄水变量情况的集水面积相差在20%至1倍之间情形;径流系数法适用于集水面积相差在20%至1.5倍之间且估算时段有洪水过程情形;区间水量平衡法适用于上下游参证水文站面积比在3倍以内情形;“两种方法相对偏差小于18%”的估算成果合理性分析标准可行。     

黔西南水银洞金矿床流体包裹体研究

水银洞金矿床是黔西南典型的特大型卡林型金矿床之一。但其成矿流体来源尚有争议。通过对水银洞金矿床脉石英和方解石中流体包裹体的温度与压力、盐度与密度、包裹体成分和包裹体H和O同位素等方面的研究,指出水银洞卡林型金矿床成矿流体属中低温(96.7~220℃)、低盐度(NaCl)0.635%~9.861%,平均值为4.282%±2.260%、中等密度(0.725~0.977 g/cm3,平均值为0.910±0.061 g/cm3);脉石英阶段流体水化学类型属Cl--Na+型或SO42--Cl-Na+型,方解石阶段属SO42--Cl--Ca2+型。成矿流体压力可能为高-超高压(160±40 MPa);成矿流体主要是大气降水形成的地下热水,可能有部分岩浆热液的掺入。     

The Constitute of Thick Tight Sandstone Belt and the Main Controlling Factors of Effective Reservoir

<正>1 Introduction The western 3D region of the Sulige gas field is located in the Yi-shan slope north of northern Su47 and south of Su48 medium sand belt of the Ordos Basin,the area of it is about 431 km2.The average thickness of He8 is 69.1m,the thickness of it is 83.9m;The effective sand thickness is14.4 m,the thickness of it is 21.5m.     

大型帐篷构造对贵州天星桥喀斯特石林形成演化的控制作用

贵州黄果树天星桥景区下三叠统安顺组白云岩广泛出露,分布有连片水上石林,其发育在下三叠世台地相与深海盆地过渡的台地边缘相带上,这里海水进退频繁,古海岸带暴露面上大型帐蓬构造十分发育.碳酸盐岩古暴露带这种岩性特征,一则导致岩石充满裂隙及孔洞,极有利于大气降水入渗及地下水的赋存和运移;另则导致岩体软弱结构面密布,岩石的力学强...     

碳化对加填料氯氧镁胶凝材料力学性能的影响

以添加不同掺量填料的氯氧镁水泥(MOC)试件为基体,在空气和碳化箱中分别养护至规定龄期,研究碳化对掺加填料MOC力学性能的影响,并与空气环境进行对比,运用XRD和SEM分析碳化后水化产物和微观结构的变化规律。结果表明,28 d龄期内,随着填料掺量的增加MOC试件碳化后的抗折强度均有所增加,碳化后的抗压强度均有提升,碳化过程反应生成了MgCO_3,不同碳化龄期的主要物相均为5Mg(OH)_2·MgCl_2·8H_2O(5相),而掺加填料不影响MOC的水化产物的组成。     

硒在干酪根中的两种不同赋存状态:TEM证据

对拉尔玛Se—Au矿床和渔塘坝硒矿床中干酪根的高分辨透射电镜(TEM)研究表明，两个矿床的Se在干酪根中的赋存状态是截然不同的。前者Se在干酪根中的富集可能主要以有机结合态的形式存在，主要是取代干酪根中的硫；而后者Se在干酪根中的富集主要以超微包体Se的形式吸附在干酪根中。两种不同的赋存状态可能主要取决于干酪根中硫含量的多少以及成矿时氧化还原条件的变化。研究表明，TEM是研究元素在干酪根中赋存状态的有效手段。     

陆面露水资源开发利用技术初探

简要归纳了陆面露水资源独特的生态和气候效应,分析了开发利用陆面露水资源的理论依据,并重点从局地气候条件的选择和陆水凝结面的改进2个方面讨论了陆面露水资源开发利用的科学思路和技术措施。