Experimental study on reduction of U (Ⅵ) by an anaerobic bacterium, Shewanella putrefaciens: Application to sandstone-hosted interlayer oxidation-zone type uranium deposits, China

An experimental study on reduction of U (Ⅵ) by anaerobic bacteria, Shewane//a putrefaciens, is first reported here in China. The experimental conditions were: 35℃ and pH =7.0-7.4, corresponding to a physicochemical environments in which the sandstone-hosted interlayer oxidation-zone type uranium deposit formed in Northwest China's Xinjiang. Bacteria adopted in the present experiment, Shewanella putrefaciens, occur extensively in natural environment. Our study shows that nano-crystal precipitates of uraninite quickly occurred on the surface of the cells within one week. It was found that the pitchblende was characterized by a random arrangement of uraninite nanocrystals (2-4 nm) in it, significantly different from natural pitchblende in which uraninite nanocrystals are arranged in order. Finally, a possible mechanism of uranium biomineralization by microorganisms in the deposits is discussed. Our investigation may supply a technical train of thoughts for bioremediation of nuclear-contaminated water environments and for underground dissolving extraction of the sandstone-hosted uranium ores.     

Soil respiration in tropical seasonal rain forest in Xishuangbanna, SW China

With the static opaque chamber and gas chromatography technique, from January 2003 to January 2004 soil respiration was investigated in a tropical seasonal rain forest in Xishuangbanna, SW China. In this study three treatments were applied, each with three replicates: A (bare soil), B (soil+litter), and C (soil+litter+seedling). The results showed that soil respiration varied seasonally, low from December 2003 to February 2004, and high from June to July 2004. The annual average values of CO2 efflux from soil respiration differed among the treatments at 1% level, with the rank of C (14642 mgCO2· m-2. h-1)＞B (12807 mgCO2· m-2. h-1)＞A (9532 mgCO2· m-2. h-1). Diurnal variation in soil respiration was not apparent due to little diurnal temperate change in Xishuangbanna. There was a parabola relationship between soil respiration and soil moisture at 1% level. Soil respiration rates were higher when soil moisture ranged from 35% to 45%. There was an exponential relationship between soil respiration and soil temperature (at a depth of 5cm in mineral soil) at 1% level. The calculated Q1o values in this study,ranging from 2.03 to 2.36, were very near to those of tropical soil reported. The CO2 efflux in 2003was 5.34 kgCO2· m-2. a-1 from soil plus litter plus seedling, of them 3.48 kgCO2· m-2. a-1 from soil (accounting for 62.5%), 1.19 kgCO2· m-2. a-1 from litter (22.3%) and 0.67 kgCO2·m-2. a-1 from seedling (12.5%).     

Study of statistically correcting model CMAQ-MOS for forecasting regional air quality

Based on analysis of the air pollution observational data at 8 observation sites in Beijing including outer suburbs during the period from September 2004 to March 2005, this paper reveals synchronal and in-phase characteristics in the spatial and temporal variation of air pollutants on a city-proper scale at deferent sites; describes seasonal differences of the pollutant emission influence between the heating and non-heating periods, also significantly local differences of the pollutant emission influence between the urban district and outer suburbs, i.e. the spatial and temporal distribution of air pollutant is closely related with that of the pollutant emission intensity. This study shows that due to complexity of the spatial and temporal distribution of pollution emission sources, the new generation Community Multi-scale Air Quality (CMAQ) model developed by the EPA of USA produced forecasts, as other models did, with a systematic error of significantly lower than observations, albeit the model has better capability than previous models had in predicting the spatial distribution and variation tendency of multi-sort pollutants. The reason might be that the CMAQ adopts average amount of pollutant emission inventory, so that the model is difficult to objectively and finely describe the distribution and variation of pollution emission sources intensity on different spatial and temporal scales in the areas, in which the pollution is to be forecast. In order to correct the systematic prediction error resulting from the average pollutant emission inventory in CMAQ, this study proposes a new way of combining dynamics and statistics and establishes a statistically correcting model CMAQ-MOS for forecasts of regional air quality by utilizing the relationship of CMAQ outputs with corresponding observations, and tests the forecast capability. The investigation of experiments presents that CMAQ-MOS reduces the systematic errors of CMAQ because of the uncertainty of pollution emission inventory and improves the forecast level of air quality. Also this work employed a way of combining point and area forecasting, i.e. taking the products of CMAQ for a center site to forecast air pollution for other sites in vicinity with the scheme of model products "reanalysis" and average over the "area".     

Chemical forms of heavy metal contaminants in sediments of Miyun reservoir

The chemical forms, spatial distribution and sources of As, Hg, Cd, Pb and Zn in sediments of the Miyun reservoir were studied. The results of sequential extraction demonstrate that most of As, Pb and Zn were bound to the residual fraction, Hg was associated with the sulfide fraction while Cd was associated with the carbonate fraction and the residual fraction. On the vertical profiles the concentrations of the heavy metals in total and each fractions mostly decreased with increasing depths in sediments, suggesting that the heavy metals input from the upstream watershed increases yearly. Summation of the residual fraction, the sulfide fraction and the carbonate fraction accounts for 60.03%―85.60% of the total heavy metal contents in the sediments, which represent the geochemical background values of the elements and relate closely to soil erosion. Results of the main factor analysis show that most sediments of the reservoir come from the upstream soil erosion, the point source pollution and domestic waste. Moreover, the microbial activities taking place on the sediment-water interface are also one of the major factors to cause the increasing content of the organic matter fraction and the iron-manganese oxide fraction. Environmental change of the reservoir water could make the removability of the heavy metals increase, leading to the increase of their concentrations in pore water in sediments, and imperiling water quality of the reservoir.     

沙坡头没蒿和柠条叶片δ13C 和 N 含量的季节变化特征

通过对腾格里沙漠东南缘沙坡头人工固沙区油蒿和柠条叶片稳定碳同位素分辨率（△）和N 含量的测定，研究了不同种植方式下油蒿和柠条叶片△ 和 N 含量的季节变化及其关系。结果表明：在不同种植方式下，两种植物的△ 与 N 含量在不同月份之间均存在显著差异。油蒿叶片 △ 极显著高于柠条的：在不同种植方式下，油蒿单种下 △ 显著高于混种，柠条则反之。 油蒿叶片 N 含量极显著低于柠条的：不同种植方式下柠条叶片 N 含量无显著差异，而单种油蒿叶片 N 含量则显著低于混种。在 △ 与 N 含量的关系中，柠条叶片 △ 与 N 在两种生境、单种和混种下均极显著正相关，雨明柠条叶片 N 含量可以作为其 △ 与 N 含量的季节变化及其关系在不同微生境有所差异。     

空间运动对象与时空数据类型研究

当前时空数据模型多以描述空间实体的离散变化为主。该文中对空间运动对象在抽象层次的无限连续空间、离散层次的有限离散空间上的数据类型进行分析和定义，将其分别划分为时间类型、空间类型和时态类型来研究，并提出支持空间运动对象的表示方法和操作方式。该方法既能表示空间实体的连续运动，也能表示其离散变化，为空间运动对象时空数据模型的建立奠定了基础。     

基于高通量测序的表层水体纤毛虫多样性评估方法比较与优化

基于扩增子的高通量测序技术广泛应用于微型生物多样性的研究, 不同的测序手段和数据分析流程, 影响着微型生物多样性与群落结构的分析结果。本研究以易于形态鉴定的砂壳纤毛虫作为研究对象, 比较DNA测序、RNA测序和形态学方法检获的多样性和物种组成等, 探究序列分析流程中关键步骤: 嵌合体处理、可操作分类单元分析方法选择、合并相似分类单元以及去除稀有类群等对多样性结果的影响。研究结果显示无论基于DNA还是RNA的分子手段与形态学方法检获的主要物种基本一致, 与DNA测序相比, RNA测序检获的物种数少, 但差异不显著。基于97%以上相似度聚类和单核苷酸变异所得群落结构相似, 无显著差异; 且所有分析方法都能在一定程度上反映出自然界中不同类群的相对丰度。相较于单核苷酸变异和其他相似度阈值, 99%相似度下聚类所得多样性更为接近形态学结果。去除嵌合体和稀有类群(去除阈值: DNA测序0.05%; RNA测序0.07%), 可明显改善分子多样性虚高的问题。本研究为纤毛虫等真核微生物的分子多样性研究提供了科学的数据分析流程, 对未来真核微生物多样性研究具有指导意义。     

基于小波包变换特征向量提取的隐蔽水声通信性能评价方法研究

针对水声通信系统的隐蔽性缺乏客观、有效的评价手段,提出一种基于小波包变换提取信号特征向量并比较特征向量相似度的信号隐蔽性评价算法。为了区别截获信号和环境噪声,考虑从能量分布特征的角度,提取截获信号的特征参数。所提算法利用小波包变换具有更高的时频分辨率、自适应选择频带等特点,获取信号的小波包能量占比、小波包能量熵、小波包尺度熵,采用上述 3 个特征参数组成特征向量和利用余弦相似度算法判别信号隐蔽性。仿真实验和海试结果表明,所提方法得到的信号隐蔽性能基本符合实际信号隐蔽性能随信噪比变化规律,为隐蔽水声通信系统评估设计和水声对抗性能评级提供有效参考。     

面向多区域高效搜索的UUV集群任务分配

UUV 集群在执行大范围搜索任务时分配方案的优劣对于提高任务执行效率至关重要。研究解决 UUV 集群同时进行多区域搜索中的任务分配问题,使得全部区域搜索完成时长最小。针对传统匈牙利算法无法高效解决不平衡任务分配的问题,提出一种改进匈牙利多轮分配算法。该算法通过多轮任务分配,实现空闲 UUV 高效利用和目标区域合理分配,通过在代价函数中引入边际代价和保守估计时长,大大减小了全部区域搜索完成时长。仿真实验结果表明：提出的算法相比传统匈牙利算法能够合理分配空闲 UUV,提高分配效率。此外,相较于仅采用搜索时长为代价函数,在代价函数中引入边际代价和保守估计时长能够针对耗时长的区域最大限度缩短搜索时长,保证随着 UUV 数量的增加,全部区域搜索完成时长单调递减。     

能见度集合预报及后处理技术应用

基于污染物情况、环流系统和时空分布特征分析,利用神经网络对历史数据进行建模,生成了能见度集合预报产品。在2022年冬季的TS评分检验中,预报产品优于欧洲中期数值预报中心模式(ECMWF)的能见度预报产品。利用概率匹配、最优百分位和神经网络三种后处理方法生成后处理产品,这些产品的TS评分优于集合预报产品。预报输入的ECMWF模式2 m湿度与实况的偏差是误差的主要来源。利用集成方法对三种后处理产品进行集成,其TS评分结果在低能见度区间总体接近或略优于原始产品。生成的能见度集合预报后处理最优集成预报产品成功提高了对中期延伸期能见度天气的预测准确性。