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121.
山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析 总被引:26,自引:7,他引:26
采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场,各20尾,取全血或肌肉组织,以酚-仿抽提方法提取基因组DNA,利用筛选获得的10对微卫星引物进行PCR扩增,反应产物经8%非变性聚丙烯酰胺凝胶电泳分离、EB显色,用ImageMasterl D Elite(Version 3.01)软件分析电泳结果,并计算了相应的遗传学参数。结果表明,在自然和养殖群体中,10个基因座位的等位基因平均数(α)分别为6.7和6.1,每个基因座位有效等位基因数(αe)分别为1.8—6.8和2.5—6.7,群体平均杂合度(H)分别为0.8120和0.7310;两个群体间的遗传相似性系数、遗传距离和基因分化系数为0.8558、0.1557和0.0558;自然群体内每个座位上的多态信息含量(PIC)为0.59—0.84、个体识别率(DP)为0.54—0.86、非父排除率(PPE)为0.41—0.72,其累积个体识别率和非父排除率均达到0.9999,表明所选座位属中高识别力的遗传标记,可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。 相似文献
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我国的渤海和黄海北部在每年冬季都出现不同程度的冰情,它直接影响结冰海区的石油平台、船舶以及港口等设施的正常作业,对海冰的观测与预报随着上述海区的开发利用越发显得重要,利用航海雷达连续准确地跟踪海冰漂移运动轨迹,是当今观测、调查、研究冰漂流移动规律的有效方法之一,我国的科研人员利用雷达成像技术对渤海的冰情进行了长期的雷达海冰观测、研究,在由雷达海冰图像对海冰的物理特征的识别、分类以及冰漂流场测量方面取得了一些进展[1-4]。 相似文献
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鲽形目3亚目8科51属142种。鲀形目2亚目9科57属143种。海蛾鱼目1科2属3种,鮟鱇目3亚目11科23属41种。 相似文献
126.
旅游生态效率是反映旅游经济与旅游环境双向效应(bi-effect)的重要判断指标。本文基于非期望产出视角,采用超效率SBM模型,评价了我国东部沿海18个主要滨海旅游城市的旅游生态效率,并分析了其时空演变特征。研究结果表明:(1)中国东部沿海18市滨海旅游生态效率整体呈中等水平,平均值处于0.727~0.894,阶段特征明显,呈现前期平稳发展,中期下降,后期回升的发展趋势;(2)各城市滨海旅游生态效率水平差异显著,分为下行风险型,持续成长型,稳定型和波动型4种发展类型。平均效率值最高为天津,最低为三亚;(3)分解效率均值中纯技术效率较高,规模效率次之,技术进步是影响综合效率的主要因素;(4)投入冗余和非期望产出冗余已经成为阻碍滨海旅游生态效率进步的关键因素。 相似文献
127.
Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum 总被引:1,自引:0,他引:1
Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc. 相似文献
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129.
A method combining boundary and finite element approaches as well as modal analysis to solve the acoustical scattering from elastic obstacle in infinite fluid is described. An elastic cylinder with finite length immersed in water is chosen as an example for numerical demonstration. The computed frequency response and direction pattern of the backscattering target strength of the cylinder are conformable to those measured. 相似文献
130.
Up to now, available method of numerical forecasting and suitable wind field model for the Bohai Sea storm surge have been few. In this paper, through the analysis of the weather situation is presented a mathematical model for the wind fields involved mainly the deformation field of a high pressure matched with a low pressure, the temperate cyclone, the cold wave and the northword typhoon. Meanwhile, numerical computations combined with the nonlinear storm surge models are made by using "ADI" method. The computed results are generally coincident with the practical observations. It has showed a success in the simulated wind field and the feasibility of using "ADI" method to forecast the Bohai Sea storm surge. 相似文献