蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

Protective effect of topically applied polypeptide from Chlamys farreri against ultraviolet radiation-induced chronic skin damage in guinea pig

Polypeptide fromChlamys farreri (PCF), a topical polypeptide isolated fromChlamys farreri, was used in this experiment aimed to investigate the photoprotective effect of PCF against chronic skin damage induced by ultraviolet A (UVA) and ultraviolet B (UVB) radiation. The chronic ultraviolet-irradiated guinea pig model was established, and visible changes in the skin including wrinkling, sagging and erythema were observed. Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in the dorsal skin were determined using biochemical methods. The results showed: (1) PCF (5 % and 20%) could greatly protect the dorsal skin of guinea pig against wrinkling, sagging and erythema induced by UV radiation in a concentration-dependent manner. (2) PCF could reduce MDA formation in the dorsal skin caused by UV irradiation, while increasing the activities of SOD and GSH-px. (3) The differences among the PCF groups and UV model group were significant (P<0.05,P<0.01). These results indicated that topical application, of PCF provided broad solar UV spectrum photoprotection; and that the antioxidant property of PCF might play a role in photoprotection. Project No. 39970638 supported by the NSFC, and the Science and Technology Bureau of Qingdao (No. 2001-28-50).     

小行星(360)Carlova自转参数的测定

小行星自转参数的资料不仅可以为小行星碰撞演化提供数据,还可以为太阳系的演化研究提供依据．利用云南天文台1米望远镜对主带碳类小行星(360)Carlova进行了新的CCD测光观测,结合前人的测光资料,利用Epoch-方法对(360)的自转参数进行了反演计算,得到该小行星自转的恒星周期为0．25780417±0．00000003天,自转轴的黄道坐标为(95°±3,°40°±1°)．新的结果与前人的结果相比较为一致,其精度稍高于以前的估算结果．     

胶东“玲珑-焦家式”金矿资源潜力与找矿

“玲珑焦家式”金矿的成岩成矿,从新太古代至中生代存在着继承性的成因关系,成矿具有长期性、多源性特点,但中生代燕山早期岩浆活动是主导成矿因素。区域化探成果显示胶东金矿直接矿源岩———郭家岭花岗岩金的丰度比原始矿源岩———早前寒武纪变质沉积岩系及侵入岩系金的丰度低1/2。郭家岭花岗岩面积在2 km2以上岩体定量估计金亏损总量与胶东金矿总量预测的资源量相当,从一个侧面展现了胶东金矿资源的潜力。望儿山金矿的找矿实践展示,焦家式金矿在1 000 m以下尚有巨大潜力,整个胶东金矿产区在3 000 m以上的资源潜力应在5000 t以上。     

上扬子地台震旦系铅锌矿床类型及找矿方向

上扬子地台含铅锌矿地层主要为震旦系。震旦系在上扬子地台主要发育上统灯影组,次有下统陡山沱组。将上扬子地台震旦系铅锌矿床成因划分为一级为叠生矿床,二级为层控矿床,三级为热水沉积改造矿床和热水沉积再造矿床四级类型,该类型按铅锌比例进一步划分为富铅型、富锌型、铅锌共生型。研究区内最重要的铅锌矿床成因类型为南郑马元、会东大梁子铅锌矿为代表的富锌型。陕西南郑、紫阳—镇坪地区,湖北武当、神农架、黄陵地区,以及地台西缘的安宁河、甘洛—小江、峨边—寻甸等地区的震旦系是寻找铅锌矿的最有利地段。     

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …     

CCD联合平差方法及其初步实例检验

在常规照相天体测量工作中 ,对暗天体的定位是采用逐级定标星过渡的方法实现的。由于星表系统差和局部差的存在 ,最终定位结果可能包含难以把握的误差积累。在CCD小视场观测情况下时常难以找到足够数量的定标星。鉴于此 ,推导了CCD观测联合平差方法的严格矢量表达式 ,以期通过共同星的过渡 ,实现大天区统一平差。原理上此方法可实现相对于河外天体的定位 ,以削弱星表系统差和局部差对定位结果的影响。讨论了CCD联合平差方法实现中的问题 ,包括底片常数模型选择、切点位置改正、共同星的较差改正和法方程解算方法等 ,并分析了具体的处理措施。用实例初步检验了CCD联合平差方法的效果 ,表明通过对多张CCD观测的联合平差 ,可以削弱单张CCD观测参考星数目过少、单张CCD观测参考星分布不均、个别参考星存在位置偏差和局部区域参考星存在系统性位置偏差等不利因素对归算结果的影响 ,进而达到扩大视场和提高归算精度的目的     

板翅式换热器综合性能优化的设计方法

应用给定换热器结构材料而使换热量最大的两侧换热表面的最佳匹配准则和使可用能损失率最小的最佳运行参数准则。利用两个准则间的关系 ,采用迭代的方式完成换热器的优化设计 ,使换热器的设计达到材料省、换热效果好及运行费用低的目的 ,且能在设计阶段实现。     

Geological environment conflicts of Kunming Basin and its urban sustainable development

Kunming Basin locates middle of Yunnan altiplano and has a particularity in geography,topographic and geological environment.With the urban dilation quickly,add the reason of the unreasonable city layout,conflicts between environment and urban resources consumption become shrill increasingly.It is human being activities that lead to vulnerability and depravation of geological environment in local.Take a few examples on geological environment to expatiate relationship between urban construction and geological environment carrying capacity,and find a way how to make a better plan for urban sustainable development to achieve new balance between man and nature in local.