日本鳗鲡早期阶段耳石日生长轮形成的周期

于1990年4月从江苏太湖搜集日本鳗鲡亲鱼暂养在天津滨海虾场8t玻璃钢水槽中，经人工催熟催产后孵出仔鳗，对孵出仔鳗连续取样；于1989年4月在长江口采集白仔鳗，观察和比较二者的耳石生长轮形成。结果表明，（1）人工繁殖仔鳗耳石第一个生长轮是在孵出后第一天形成的，轮纹形成具有24h周期性；仔鳗孵出后生长天数与生长轮数关系的回归方程以y=0．23＋0．91x表示，其中，x为孵出后的天数，y为生长轮数;在人工繁殖仔鳗耳石上没有观察到“孵化标记”轮。（2）白仔鳗耳石中心核与“孵化标记”轮之间存在日生长轮。（3）人工繁殖仔鳗与白仔鳗耳石中心核同第一个生长轮比较表明，前者小于后者。     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.     

羊栖菜多糖提取与含量的测定

通过水提醇沉方法提取羊栖菜粗多糖,纯化后测定羊栖菜中多糖含量,为今后羊栖菜化学及药理研究提供有益的参考.采用了分光光度法、苯酚-硫酸显色法在490 nm处测定其含量.结果表明,羊栖菜提取液中多糖含量达53.46%,平均多糖回收率为99.37%.证明该方法实验操作简便,准确可靠,可用于羊栖菜多糖含量的测定.     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

江苏岸外辐射沙脊群东沙稳定性研究

东沙是江苏岸外辐射沙脊群中的第二大沙洲,具有独特的地形地貌和水动力条件,对它进行稳定性研究为揭示整个辐射沙洲及其邻近岸滩的动态演变都非常有益。通过利用多年遥感卫片资料、1998年取得的现场水文泥沙观测资料和东沙滩面表层沉积物资料等,对东沙的地形地貌特征、沉积特征和东沙两侧潮汐通道的水流泥沙特征等进行了详细分析。研究结果表明,东沙的沙脊偏于西侧,西侧滩面较窄、高程较高且岸线较为顺直,东侧滩面较宽、高程较低且岸线较为破碎;西洋和陈家坞槽均处于冲刷状态,净输沙的主要方向为输向槽外或输向条子泥;东沙近三十年来面积有所缩小且有外围向中央收缩的趋势,尤其以向东、向南方向的迁移最为明显。     

南黄海夏季海水pCO2研究Ⅱ--下层海水涌升和长江冲淡水对海-气界面CO2通量的贡献

根据2001年7月对南黄海的大面积调查,研究了南黄海夏季pCO2的分布机制,着重讨论下层海水涌升和长江冲淡水对海-气界面CO2通量的贡献,并给出了南黄海海-气界面CO2通量。研究结果表明:夏季南黄海总体上是CO2的1个弱源,大约向大气中释放45.05×104t C。夏季南黄海表层海水pCO2分布表现出了极大的不均性,其汇区主要由长江冲淡水造成,影响区域占汇区吸收CO2的99.9%;而在源区,下层海水涌升虽然面积较小却占源区释放CO2的35.2%。可见陆架边缘海区源/汇格局的地域差异非常之特别。     

台湾海峡野生牙鲆人工育苗技术研究

报道了2004年3月对产自台湾海峡的野生牙鲆(Paralichthys olivaceus)进行人工育苗的研究结果。共获153万粒受精卵,孵出仔鱼140.8万尾,孵化率达92%,在育苗水温为20~24℃,海水盐度为26~31的条件下,经63 d培育,育出全长3.8~5.5 cm的幼鱼53.39万尾,成活率为37.9%。     

Studies on Genus Cryptomonas from China seas

ＩＮＴＲＯＤＵＣＴＩＯＮＣｒｙｐｔｏｍｏｎａｓｉｓｕｎｉｃｅｌｌｕｌａｒｂｉｆｌａｇｅｌｌａｔｅｗｈｉｃｈｉｓａｃｏｍｍｏｎｇｅｎｕｓｏｆＣｒｙｐｔｏｐｈｙｔａ .Ｔｈｅｃｅｌｌｓａｒｅｕｓｕａｌｌｙｓｔｒｏｎｇｌｙｃｏｍｐｒｅｓｓｅｄｌａｔｅｒａｌｌｙ .ＴｈｅｒｅａｒｅｏｎｅｏｒｔｗｏｌａｒｇｅＣｈｌｏｒｏｐｌａｓｔｓｔｈａｔｃｏｎｔａｉｎｃｈｌｏｒｏｐｈｙｌｌａ ,ｃ ,ｓｏｍｅｃｈａｒａｃｔｅｒｉｓｔｉｃｃａｒｏｔｅｎｏｉｄｓａｎｄｐｈｙｃｏｂｉｌｉｎｐｉｇｍｅｎｔｓ (Ｈｕｅｔａｌ.,1 997;Ｗｅｉ…     

鱿鱼精核蛋白提取物的保健功能研究

以鱿鱼精巢组织中提取的核蛋白为主要成分制成精核蛋白提取物 ,利用功能学评价实验方法对提取物进行抗疲劳、抗氧化以及免疫调节实验。结果表明 :( 1 )该提取物能明显延长小鼠爬杆和负重游泳时间 ,减少小鼠运动时肝糖元的消耗 ,降低运动后血乳酸的水平 ;( 2 )该提取物能降低血中过氧化脂质降解产物丙二醛 ( MDA)含量、增强血中超氧化物歧化酶( SOD)活力 ;提高小鼠 NK细胞活性、巨噬细胞吞噬百分比和吞噬指数及细胞免疫水平。因而 ,该提取物有抗疲劳、抗氧化以及免疫调节作用