Crude oil has been discovered in the Paleogene and Neogene units of the Weixinan Sag in the Beibu Gulf Basin. To determine the source and accumulation mode of this crude oil, 12 crude oil samples and 27 source rock samples were collected and an extensive organic geochemical analysis was conducted on them. Based on the geological conditions and the analytical results, the types, origins and accumulation patterns of crude oil in the study area were elucidated. Except for a shallowly-buried and biodegraded crude oil deposit in Neogene rocks, the crude oil samples in the study area were normal. All of the crude oils were derived from lacustrine source rocks. According to biomarker compositions, the crude oils could be divided into two families, A and B, distinctions that were reinforced by differences in carbon isotope composition and spatial distribution. Oil-source correlation analysis based on biomarkers revealed that Family A oils were derived from the mature oil shale at the bottom of the second member of the Liushagang Formation, while the Family B oils formed in the mature shale of the Liushagang Formation. The Family A oils, generated by oil shale, mainly migrated laterally along sand bodies and were then redistributed in adjacent reservoirs above and below the oil shale layer, as well as in shallow layers at high structural positions, occupying a wide distribution range. The Family B oils were generated by other shale units before migrating vertically along faults to form reservoirs nearby, resulting in a narrow distribution range. 相似文献
Although biofilm formation may promote growth, biofilms are not always beneficial to their hosts. The biofilm formation characteristics of Bacillus cereus WPySW2 and its changes at different temperatures were studied. Results show that B. cereus WPySW2 promoted the growth of Neoporphyra haitanensis (an economically cultivated seaweed) at 20 °C but accelerated algal rot at 28 °C. Thicker B. cereus WPySW2 biofilms covered the surface of N. haitanensis thalli at 28 °C, which hindered material exchange between the algae and surrounding environment, inhibited algal photosynthesis and respiration, and accelerated algal decay. Compared with planktonic bacteria, mature biofilm cells had lower energy consumption and metabolic levels. The biofilm metabolic characteristics of B. cereus WPySW2 changed significantly with temperature. High temperature accelerated biofilm maturation, which made it thicker and more stable, allowing the bacteria to easily adapt to environmental changes and obtain greater benefits from their host. High temperature did not affect the production or increased the abundance of toxic metabolites, indicating that the negative effects of B. cereus WPySW2 on algae were not caused by toxins. This study shows that increased temperature can transform a harmless bacterium into a detrimental one, demonstrating that temperature may change the ecological function of phycospheric bacteria by affecting their morphology and metabolism.