Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

白垩纪温度较冷的估价

白垩纪温度较冷的估价Ｂ．ｗ．Ｓｅｌｌｗｏｏｄ等有关白垩纪气候的概念部分基于详尽的地质证据，如不耐寒冷的古生物（如：珊瑚的分布比现在偏极地５－１５℃）和特殊气候条件的沉积相的广泛分布。这些资料的解释反映２１℃的等温线在纬度上至少向两极移动５°，全球的平...     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

Using benthic macrofauna to assess environmental quality of four intertidal mudflats in Hong Kong and Shenzhen Coast

Abstract-Intertidal zone is a significant wetland between land and ocean. It plays an important rolein maintaining local ecological balance. Both Mai Po and Futian intertidal mudflats are located in Shen-zhen Bay and are important "refueling" point along the East Asian/Australian flyway of migratory birds.The environmental quality of Mai Po and Futian mudflats have aroused great concern due to rapid eco-nomic developments in Hong Kong and Shenzhen in recent decades. Microinfauna of Mai Po and Futianmudflats was investigated in December 2000 and the faunal data were used to assess their environmentalquality. Two other mudflats, namely Ma Wan Typhoon Shelter (a more disturbed area) and Luk Kengintertidal mudflat (a place with relatively less human disturbance), were also sampled for macroinfaunaand used as reference mudflats. Shannon-Weaver species diversity index (I_(sd)), biotic coefficient (C_b)and macrofaunal pollution index (I_(mp)) of the macrofauna community on four intertidal mudflats wereused to     

美国飓风暴潮预报

美国国家天气局已发展了一种飓风暴潮模式,即SLOSH模式,用其计算飓风暴潮,给定风暴资料,做为输入参数。这个数值模式把动力海岸线、由风暴引起的洪水漫滩、次网格特征具体化,如障碍物、障碍物之间的缺口、海水沿可变宽度河道的一维流动。SLOSH模式已被用于墨西哥湾沿岸美国的大部分地区和大西洋海岸常遭飓风袭击的区域。本文提供了模式使用的情况和对于飓风暴潮预报的某些局限性。SLOSH模式应用的一些特例取自1985年Elena飓风。 SLOSH模式除了用于实时风暴潮预报外,还广泛用于飓风防汛疏散计划。这个模式用几百个假想的飓风进行运算,是依据区域气候学来选择所计算的飓风,对每个预想的风暴,模式计算出淹水范围,把这些淹水类型结合起来,可帮助确定一个区域遭飓风袭击的程度。同时,模式计算的风,可帮助规划人员确定疏散时路线,是否由于大风而阻塞。联邦和地方政府机构把这些息信与人口研究和道路能力估计结合起来,制定出一种综合的疏散计划。这种计划的成果之一是“疏散时间”——当飓风来临时沿海地区为了安全疏散需要提前撤离的时间。     

粉红色红拟抱球虫在冲绳海槽区的发现及地层意义

近年来,我们在冲绳海槽区进行大量工作.本文就Z14-6孔(27°07′N127°27′E)首次发现粉红色红拟抱球虫,及稳定同位素测定,进行对比研究,证明了Thompson等1979年所提出的粉红色拟抱球虫在印度-太平洋海域灭绝时代在距今120000年左右的观点.     

碳同位素质谱法探讨现代海洋有机沉积物来源

本文用NUCLIDE RMS 6-60质谱仪测定了长江口及邻近海区(北纬30°06′至31°15′,东经122°30′至124°30′)表层沉积物中有机碳稳定同位素组成(δ~(13)C为-23.6×10~(-3)—-21.3×10~(-3))、有机碳含量(0.4—0.1％),溶剂可提取有机物浓度及有机C/N比值。结果表明,从有机地球化学角度解释并证实了其他地学家在该区域的研完成果,即东海陆架泥质沉积物主要由陆源悬浮物和海洋浮游生物体混合组成,长江入海物质随着远离长江口而递减,大部分陆源悬浮物向东南运移,并沉积在东经123°以西的长江口区。     

重金属对海洋围隔生态系中初级生产力的影响

1985年4月,中国和加拿大科学家首次在厦门合作进行添加重金属(Cu,Hg,Zn,Pb,Cd)的围隔生态系实验,实验是在海边大水池内的塑料袋中进行,实验结果说明低水平(ppb)混合重金属对浮游植物的影响首先表现在对光合作用速率的抑制作用,以及在浓度较低的情况下可能引起的种群演替现象,本文还阐述了颗粒有机碳(POC)和颗粒有机氮(PON)与初级生产力的相关性.