Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

Wide-field VLBI imaging

We discuss the technique of Wide-field imaging as it applies to Very Long Baseline Interferometry (VLBI). In the past VLBI data sets were usually averaged so severely that the field-of-view was typically restricted to regions extending a few hundred milliarcseconds from the phase centre of the field. Recent advances in data analysis techniques, together with increasing data storage capabilities, and enhanced computer processing power, now permit VLBI images to be made whose angular size represents a significant fraction of an individual antenna's primary beam. This technique has recently been successfully applied to several large separation gravitational lens systems, compact Supernova Remnants in the starburst galaxy M82, and two faint radio sources located within the same VLA FIRST field. It seems likely that other VLBI observing programmes might benefit from this wide-field approach to VLBI data analysis. With the raw sensitivity of global VLBI set to improve by a factor 4–5 over the coming few years, the number of sources that can be detected in a given field will rise considerably. In addition, a continued progression in VLBI's ability to image relatively faint and extended low brightness temperature features (such as hot-spots in large-scale astrophysical jets) is also to be expected. As VLBI sensitivity approaches the μJy level, a wide-field approach to data analysis becomes inevitable.     

Change analysis of rice area and production in China during the past three decades

Rice＇s spatial-temporal distributions, which are critical for agricultural, environ- mental and food security research, are affected by natural conditions as well as socio-eco- nomic developments. Based on multi-source data, an effective model named the Spatial Production Allocation Model （SPAM） which integrates arable land distribution, administrative unit statistics of crop data, agricultural irrigation data and crop suitability data, was used to get a series of spatial distributions of rice area and production with 10-km pixels at a national scale -it was applied from the early 1980s onwards and used to analyze the pattern of spatial and temporal changes. The results show that significant changes occurred in rice in China during 1980-2010. Overall, more than 50% of the rice area decreased, while nearly 70% of rice production increased in the change region during 1980-2010. Spatially, most of the increased area and production were in Northeast China, especially, in Jilin and Heilongjiang; most of the decreased area and production were located in Southeast China, especially, in regions of rapidly urbanization in Guangdong, Fujian and Zhejiang. Thus, the centroid of rice area was moved northeast approximately 230 km since 1980, and rice production about 320 km, which means rice production moved northeastward faster than rice area because of the significant rice yield increase in Northeast China. The results also show that rice area change had a decisive impact on rice production change. About 54.5% of the increase in rice pro- duction is due to the expansion of sown area, while around 83.2% of the decrease in rice production is due to contraction of rice area. This implies that rice production increase may be due to area expansion and other non-area factors, but reduced rice production could largely be attributed to rice area decrease.     

XMM–Newton observations of bright ROSAT selected active galactic nuclei with low intrinsic absorption

We present a sample of 21 ROSAT bright active galactic nuclei (AGNs), representing a range of spectral classes, and selected for follow-up snapshot observations with XMM–Newton . The typical exposure was between 5 and 10 ks. The objects were primarily selected on the bases of X-ray brightness and not on hardness ratio; thus the sample cannot be strictly defined as a 'soft'sample. One of the main outcomes from the XMM–Newton observations was that all of the AGN, including 11 type 1.8–2 objects, required low levels of intrinsic absorption  ( N _H≲ 10²¹ cm⁻²)  . The low absorption in type 2 systems is a challenge to account for in the standard orientation-based unification model, and we discuss possible physical and geometrical models which could elucidate the problem. Moreover, there does not appear to be any relation between the strength and shape of the soft excess, and the spectral classification of the AGN in this sample. We further identify a number of AGN which deserve deeper observations or further analysis: for example, the low-ionization nuclear emission regions (LINERs) NGC 5005 and NGC 7331, where optically thin thermal and extended emission is detected, and the narrow-line Seyfert 1 II Zw 177, which shows a broad emission feature at ∼ 5.8 keV.     

Flux-Rope Twist in Eruptive Flares and CMEs: Due to Zipper and Main-Phase Reconnection

The nature of three-dimensional reconnection when a twisted flux tube erupts during an eruptive flare or coronal mass ejection is considered. The reconnection has two phases: first of all, 3D “zipper reconnection” propagates along the initial coronal arcade, parallel to the polarity inversion line (PIL); then subsequent quasi-2D “main-phase reconnection” in the low corona around a flux rope during its eruption produces coronal loops and chromospheric ribbons that propagate away from the PIL in a direction normal to it. One scenario starts with a sheared arcade: the zipper reconnection creates a twisted flux rope of roughly one turn (\(2\pi \) radians of twist), and then main-phase reconnection builds up the bulk of the erupting flux rope with a relatively uniform twist of a few turns. A second scenario starts with a pre-existing flux rope under the arcade. Here the zipper phase can create a core with many turns that depend on the ratio of the magnetic fluxes in the newly formed flare ribbons and the new flux rope. Main phase reconnection then adds a layer of roughly uniform twist to the twisted central core. Both phases and scenarios are modeled in a simple way that assumes the initial magnetic flux is fragmented along the PIL. The model uses conservation of magnetic helicity and flux, together with equipartition of magnetic helicity, to deduce the twist of the erupting flux rope in terms the geometry of the initial configuration. Interplanetary observations show some flux ropes have a fairly uniform twist, which could be produced when the zipper phase and any pre-existing flux rope possess small or moderate twist (up to one or two turns). Other interplanetary flux ropes have highly twisted cores (up to five turns), which could be produced when there is a pre-existing flux rope and an active zipper phase that creates substantial extra twist.     

The GLE-associated flare of 21 August, 1979

We use a variety of ground-based and satellite measurements to identify the source of the ground level event (GLE) beginning near 06∶30 UT on 21 August, 1979 as the 2B flare with maximum at ～06∶15 UT in McMath region 16218. This flare differed from previous GLE-associated flares in that it lacked a prominent impulsive phase, having a peak ～9 GHz burst flux density of only 27 sfu and a ?20 keV peak hard X-ray flux of ?3 × 10^-6 ergs cm^-2s^-1. Also, McMath 16218 was magnetically less complex than the active regions in which previous cosmic-ray flares have occurred, containing essentially only a single sunspot with a rudimentary penumbra. The flare was associated with a high speed (?700 km s^-1) mass ejection observed by the NRL white light coronagraph aboard P78-1 and a shock accelerated (SA) event observed by the low frequency radio astronomy experiment on ISEE-3.     

Why chromatic imaging matters

During the last two decades, the first generation of beam combiners at the Very Large Telescope Interferometer has proved the importance of optical interferometry for high-angular resolution astrophysical studies in the near- and mid-infrared. With the advent of 4-beam combiners at the VLTI, the u ? v coverage per pointing increases significantly, providing an opportunity to use reconstructed images as powerful scientific tools. Therefore, interferometric imaging is already a key feature of the new generation of VLTI instruments, as well as for other interferometric facilities like CHARA and JWST. It is thus imperative to account for the current image reconstruction capabilities and their expected evolutions in the coming years. Here, we present a general overview of the current situation of optical interferometric image reconstruction with a focus on new wavelength-dependent information, highlighting its main advantages and limitations. As an Appendix we include several cookbooks describing the usage and installation of several state-of-the art image reconstruction packages. To illustrate the current capabilities of the software available to the community, we recovered chromatic images, from simulated MATISSE data, using the MCMC software SQUEEZE. With these images, we aim at showing the importance of selecting good regularization functions and their impact on the reconstruction.     

Molecular abundances and nucleation in protostellar envelopes

This work is basically concerned with grain nucleation occurring in protostellar envelopes. On the basis of the dissociation equilibrium theory, molecular and atomic abundances are obtained for massive protostar envelopes. Application of time-independent homogeneous nucleation theory results in the possibility of atomic Fe condensation.