Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus).

DNA from five tumors, three other-injured livers and two normal liver tissue samples from the European flounder were analyzed for mutations in exons 5-8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-ras proto-oncogenes. No tumor-specific mutations were identified by direct sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need for more extensive analyses of flounder liver tumor samples for ras or p53 mutations, other cancer-related genes should be investigated.     

Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Precipitation — elevation relationships over Sri Lanka

Summary The change of rainfall totals with height has been the subject of much investigation in many parts of the world. The rates of increase or decrease vary with the climatic regime experienced. This study of Sri Lankan rainfall shows that the relationship between height and mean annual rainfall is complex. Examples of increases and decreases of rainfall with height are found. The climatic regime of the area is important in controlling the level of maximum rainfall.With 7 Figures     

Impact of Sea-level Rise and Storm Surges on a Coastal Community

A technique to evaluate the risk of storm tides (the combination of a storm surge and tide) under present and enhanced greenhouse conditions has been applied to Cairns on the north-eastern Australian coast. The technique combines a statistical model for cyclone occurrence with a state-of-the-art storm surge inundation model and involves the random generation of a large number of storm tide simulations. The set of simulations constitutes a synthetic record of extreme sea-level events that can be analysed to produce storm tide return periods. The use of a dynamic storm surge model with overland flooding capability means that the spatial extent of flooding is also implicitly modelled. The technique has the advantage that it can readily be modified to include projected changes to cyclone behaviour due to the enhanced greenhouse effect. Sea-level heights in the current climate for return periods of 50, 100, 500 and 1000 years have been determined to be 2.0 m, 2.3 m, 3.0 m and 3.4 m respectively. In an enhanced greenhouse climate (around 2050), projected increases in cyclone intensity and mean sea-level see these heights increase to 2.4 m, 2.8 m, 3.8 m and 4.2 m respectively. The average area inundated by events with a return period greater than 100 years is found to more than double under enhanced greenhouse conditions.     

Peculiarities of Dynamic Slip Nucleation in a Thin Granular Layer

Izvestiya, Physics of the Solid Earth - Abstract—Granular media determine the dynamics of many natural systems including faults in the Earth’s crust. The paper addresses the laboratory...     

The evolution of regional development concepts: some new trends

This paper traces the theoretical development of the regional concept and the use of regionalization techniques in Soviet geography and seeks to compare this history with comparable trends in Western geography. It is severely critical of the major and rapid paradigm shifts noticed in post-war Western geographical science and relates these to shifts in the approach to global development issues.     

Manganese concentration in the silts of the Mozhaisk Reservoir

The causes of the high Mn concentration in the solid phase and pore solution of bottom sediments in the Mozhaisk Reservoir are discussed. Regularities are identified in variations the concentrations of Mn forms in reservoir deposits from its upper part to the dam.     

Expansion of the principal functions of Keplerian motion using complex variables

A special system of canonical variables is considered. An algorithm for expanding the principal functions of Keplerian motion in new elements is presented. The advantage of the proposed system is a relatively small number of terms in the classical expansions of the unperturbed two-body problem. A method for expanding the time derivatives of the rectangular coordinates is proposed. Some estimates of the number of terms in the presented expansions have been obtained through numerical experiments.     

Models of travel demand with endogenous preference change and heterogeneous agents

In the literatures of regional science, urban economics, and urban development planning, a working assumption is that individuals respond to incentives and regulations, given their preferences. Models for planning and policy analyses are used to consider what might occur if the incentives or regulations were different. In these models, however, preferences are usually assumed to be given and stable, and agents are usually assumed to be homogeneous. This paper focuses on the implications of making preferences in models of policy implementation endogenously determined and time varying heterogeneous agents. We consider first the recent literature on intertemporal choice and preference change, which cuts across many disciplines, and more briefly the literature on norm-regarding behavior. We then elaborate a simple model of transportation demand—from a static to a dynamic orientation, from fixed and exogenously given preferences of strictly self-regarding agents to endogenously determined and policy-induced preferences of heterogeneous agents—and illustrate its characteristics with simple numerical examples.     

Biosorption of Rare-Earth Elements and Yttrium by Heterotrophic Bacteria in an Aqueous Environment

This paper reports the results of the experimental determination of the coefficient of sorption of rare-earth elements and yttrium (REY) under conditions of acidic pH using several strains of heterotrophic bacteria (Microbacterium sp., Curtobacterium sp., Bacillus subtilis, Pseudomonas putida, and Bacillus pumilis) that are widespread in natural and technogenic waters of the Far East (Russia). Insignificant fractionation between heavy and light rare-earth elements, negative cerium and dysprosium anomalies, and a positive europium anomaly were revealed. The selectivity of REY biosorption by gram-positive and gram-negative bacteria, as well as inertia of the biosorption process under more acidic conditions of the medium, were shown.