Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Binary star statistics: the mass ratio distribution for very wide systems

The distribution of mass ratios for a sample of common proper motion (CPM) binaries is determined and compared with that of 798 visual binaries (VB's) studied earlier, in hopes of answering the question: Can the member stars of these systems have been drawn at random from the normal initial mass function for single stars? The observed distributions peak strongly toward q = 1·0 for both kinds of systems, but less strongly for the CPM's than for the VB's. Due allowance having been made for assorted observational selection effects, it seems quite probable that the CPM's represent the observed part of a population drawn at random from the normal IMF, while the VB's are much more difficult to interpret that way and could, perhaps, result from a formation mechanism that somewhat favors systems with roughly equal components.     

Sunspot Observations Made by Hallaschka During the Dalton Minimum

We present and analyse the sunspot observations performed by Franz I.C. Hallaschka in 1814 and 1816. These solar observations were carried out during the so-called Dalton minimum, around the maximum phase of Solar Cycle 6. These records are very valuable because they allow us to complete observational gaps in the collection of sunspot group numbers, improving the coverage for this epoch. We have analysed and compared the observations made by Hallaschka with the records made by other contemporary observers. Unfortunately, the analysis of the sunspot areas and positions showed that they are too inaccurate for scientific use. We conclude, however, that the sunspot counts made by Hallaschka are similar to those made by other astronomers of that time. The observations by Hallaschka confirm a low level of solar activity during the Dalton minimum.     

Charged isotropic model with conformal symmetry

We investigate the behaviour of a charged isotropic model with conformal symmetry. The relationship between the gravitational potentials arising from the conformal condition is used to generate a new class of exact solutions to the Einstein-Maxwell equations. A specific form of the electric field intensity and the metric potential is required to avoid a singularity at the centre. We can find simple elementary functions for the matter variables and the potentials with realistic profiles. The causality conditions, stability conditions and energy conditions are satisfied. Masses, radii, central densities and surface redshifts are generated, and the values are consistent with the compact stars 4U 1538-52 and PSR J1614-2230.     

Comets,Enceladus and panspermia

A growing body of evidence suggests the operation of life and life processes in comets as well in larger icy bodies in the solar system including Enceladus. Attempts to interpret such data without invoking active biology are beginning to look weak and flawed. The emerging new paradigm is that life is a cosmic phenomenon as proposed by Hoyle and Wickramasinghe (Lifecloud: the Origin of Life in the Galaxy, 1978) and first supported by astronomical spectroscopy (Wickramasinghe and Allen, Nature 287:518, 1980; Allen and Wickramasinghe, Nature 294:239, 1981; Wickramasinghe and Allen, Nature 323:44, 1986). Comets are the transporters and amplifiers of microbial life throughout the Universe and are also, according to this point of view, the carriers of viruses that contribute to the continued evolution of life. Comets brought life to Earth 4.2 billion years ago and they continue to do so. Space extrapolations of comets, Enceladus and possibly Pluto supports this point of view. Impacts of asteroids and comets on the Earth as well as on other planetary bodies leads to the ejection of life-bearing dust and rocks and a mixing of microbiota on a planetary scale and on an even wider galactic scale. It appears inevitable that the entire galaxy will be a single connected biosphere.     

Solar Microflares Observed by SphinX and RHESSI

In 2009, the Russian Complex Orbital Observations Near-Earth of Activity of the Sun (CORONAS-Photon) spacecraft was launched, carrying the Polish Solar PHotometer In X-rays (SphinX). The SphinX was most sensitive in the spectral range 1.2?–?15 keV, thus an excellent opportunity appeared for comparison with the low-energy end of Ramaty High Energy Solar Spectroscopic Imager (RHESSI) spectra. Common spectral measurements with these instruments cover the range where most of the flare energy is accumulated. We have chosen four consecutive small solar events observed on 4 July 2009 at 13:43 UT, 13:48 UT, 13:52 UT, and 13:55 UT (RHESSI flare peak times) and used them to compare the data and results from the two instruments. Moreover, we included Geostationary Operational Environmental Satellite (GOES) records in our analysis. In practice, the range of comparison performed for SphinX and RHESSI is limited roughly to 3?–?6 keV. RHESSI fluxes measured with a use of one, four, and nine detectors in the 3?–?4 keV energy band agree with SphinX measurements. However, we observed that SphinX spectral irradiances are three times higher than those of RHESSI in the 4?–?6 keV energy band. This effect contributes to the difference in obtained emission measures, but the derived temperatures of plasma components are similar. RHESSI spectra were fitted using a model with two thermal components. We have found that the RHESSI hot component is in agreement with GOES, and the RHESSI hotter component fits the SphinX flaring component well. Moreover, we calculated the so-called thermodynamic measure and the total thermal energy content in the four microflares that we studied. The results obtained show that SphinX is a very sensitive complementary observatory for RHESSI and GOES.     

Creating an isotopically similar Earth–Moon system with correct angular momentum from a giant impact

The giant impact hypothesis is the dominant theory explaining the formation of our Moon. However, the inability to produce an isotopically similar Earth–Moon system with correct angular momentum has cast a shadow on its validity. Computer-generated impacts have been successful in producing virtual systems that possess many of the observed physical properties. However, addressing the isotopic similarities between the Earth and Moon coupled with correct angular momentum has proven to be challenging. Equilibration and evection resonance have been proposed as means of reconciling the models. In the summer of 2013, the Royal Society called a meeting solely to discuss the formation of the Moon. In this meeting, evection resonance and equilibration were both questioned as viable means of removing the deficiencies from giant impact models. The main concerns were that models were multi-staged and too complex. We present here initial impact conditions that produce an isotopically similar Earth–Moon system with correct angular momentum. This is done in a single-staged simulation. The initial parameters are straightforward and the results evolve solely from the impact. This was accomplished by colliding two roughly half-Earth-sized impactors, rotating in approximately the same plane in a high-energy, off-centered impact, where both impactors spin into the collision.     

Sunspot Positions and Areas from Observations by Pierre Gassendi

Solar activity behaviour on the eve of the Maunder minimum may provide important information on the period of further suppression of sunspot population. We analyse sunspot positions and areas in the 1630s extracted from rare drawings published by Pierre Gassendi in Opera Omnia. This work was published in two different editions, the first in Lyon and the second almost 70 years later in Florence. The drawings published in Lyon are found to be slightly different from those published in Florence, which produces a discrepancy in the position of spots of a few degrees, while sunspot group areas may differ by a factor of two. We reveal that the orientation of the drawings in the book is not always the same as might be seen in the telescope. We conjecture that the time of Gassendi’s observations covers the beginning of a new Schwabe cycle in the southern hemisphere. The differential rotation rate in the 1630s is also assessed and discussed.