Gene Expression Analysis for Exposure to Estrogenic Substances

Zebrafish (Danio rerio) were exposed with 17β‐estradiol (E2) and nonylphenol (NP) in different concentrations. Gene expression analysis was carried out by two different approaches. First, RT‐PCR experiments were performed for the examination of expression levels of the two marker genes vitellogenin and aromatase. This approach showed a significant increase in the expression of the vitellogenin gene in exposed male fish (500 ng/L 17β‐estradiol and 250 μg/L nonylphenol). This egg yolk protein is usually not synthesized in male vertebrates. A slight decrease of expression of the aromatase gene was observed in exposed female zebrafish. Aromatase is known to catalyze the conversion of androgens to estrogens. Second, DNA microarray experiments were carried out, which allow the simultaneous examination of the expression levels of a great number of marker genes. The microarray experiments resulted in an up‐regulation of vitellogenin up to 850‐fold. In addition, several other genes were identified to be up‐regulated by estrogens, for example the high mobility group box protein ssrp1 (78‐fold) or the chaperonin containing t‐complex polypeptide 1, beta subunit cctb (22‐fold).     

Environmental Copper and Manganese in the Pathophysiology of Neurologic Diseases (Alzheimer's Disease and Manganism)

Alzheimer's disease (AD) is the most common form of dementia. Populations migrating from developing to industrialized countries seem to elicit a higher incidence and prevalence rate of AD, suggesting lifestyle and environmental factors to have a role in the pathophysiology of AD. One of its major neuropathological hallmarks is the deposition of Aβ peptides as amyloid plaques in the brain of AD patients. Aβ is proteolytically cleaved out of the larger amyloid precursor protein (APP). Cu and Mn are often found in drinking water and may have a neurotoxic potential. APP is involved in Cu homeostasis in mouse and man. In vitro observations and in vivo data obtained from APP mouse models provide strong evidence that APP overexpression enables intracellular Cu to be transported out of the cell. Disturbed metal‐ion homeostasis with elevated serum Cu levels occurs in Alzheimer and Down's patients and lowered levels in post‐mortem AD brain. We observed that bioavailable Cu has specific beneficial effects in an Alzheimer's disease mouse model. This should be regarded as a proof‐of‐concept for a prophylactic approach to overcome the observed CNS Cu deficiency in the brain of Alzheimer's disease patients. Manganism is a disorder with symptoms similar to that of Parkinson's disease. The precise mechanism how manganese can damage the nervous system is unknown. There is some evidence that iron and manganese may utilize similar transport systems. Epidemi ologic data strongly suggests that manganese enters the body primarily via inhalation and through the ingestion of manganese in drinking water.     

The Glueckstadt Graben, a sedimentary record between the North and Baltic Sea in north Central Europe

The Glueckstadt Graben is one of the deepest post-Permian structures within the Central European Basin system and is located right at its “heart” at the transition from the North Sea to the Baltic Sea and from the Lower Saxony Basin to the Rynkoebing–Fyn High.The Mesozoic to recent evolution is investigated by use of selected seismic lines, seismic flattening and a 3D structural model. A major tectonic event in the latest Middle–Late Triassic (Keuper) was accompanied by strong salt tectonics within the Glueckstadt Graben. At that time, a rapid subsidence took place within the central part, which provides the “core” of the Glueckstadt Graben. The post-Triassic tectonic evolution of the area does not follow the typical scheme of thermal subsidence. In contrast, it seems that there is a slow progressive activation of salt movements triggered by the initial Triassic event. Starting with the Jurassic, the subsidence centre partitioned into two parts located adjacent to the Triassic “core.” In comparison with other areas of the Central European Basin system, the Glueckstadt Graben was not strongly affected by additional Jurassic and Cretaceous events. During the late Jurassic to Early Cretaceous, the area around the Glueckstadt Graben was affected by relative uplift with regional erosion of the elevated relief. However, subsidence was reactivated and accelerated during the Cenozoic when a strong subsidence centre developed in the North Sea. During Paleogene and Quaternary–Neogene, the two centres of sedimentation moved gradually towards the flanks of the basin.The data indeed point toward a control of post-Permian evolution by gradual withdrawal of salt triggered by the initial exhaustion along the Triassic subsidence centre. In this sense, the Glueckstadt Graben was formed at least partially as “basin scale rim syncline” during post-Permian times. The present day Hamburger, East and Westholstein Troughs are the actual final state of this long-term process which still may continue and may play a role in terms of young processes and, e.g., for coastal protection.     

Structure and evolution of the Glueckstadt Graben due to salt movements

The salt tectonics of the Glueckstadt Graben has been investigated in relation to major tectonic events within the basin. The lithologic features of salt sections from Rotliegend, Zechstein and Keuper show that almost pure salt is prominent in the Zechstein, dominating diapiric movements that have influenced the regional evolution of the Glueckstadt Graben. Three main phases of growth of the salt structures have been identified from the analysis of the seismic pattern. The strongest salt movements occurred at the beginning of the Keuper when the area was affected by extension. This activation of salt tectonics was followed by a Jurassic extensional event in the Pompeckj Block and Lower Saxony Basin and possibly in the Glueckstadt Graben. The Paleogene–Neogene tectonic event caused significant growth and amplification of the salt structures mainly at the margins of the basin. This event was extensional with a possible horizontal component of the tectonic movements. 3D modelling shows that the distribution of the initial thickness of the Permian salt controls the structural style of the basin, regionally. Where salt was thick, salt diapirs and walls formed and where salt was relatively thin, simple salt pillows and shallow anticlines developed.     

Theory And Measurements For Turbulence Spectra And Variances In The Atmospheric Neutral Surface Layer

Predictions from a new theory for high Reynolds number turbulent boundary layers during near-neutral conditions are shown to agree well with measurements of atmospheric surface-layer variances and spectra. The theory suggests surface-layer turbulence is determined by detached eddies that largely originate in the shearing motion immediately above the surface layer; as they descend into this layer, they are strongly distorted by the local shear and impinge onto the surface. Because the origin of these eddies is non-local, they are similar to those described in previous studies as `inactive' turbulence. However, they are, in fact, dynamically highly active, supplying the major mechanism for the momentum transport, including upward bursting on the time scale of the larger eddies. The vertical velocity results show that the variance and the low frequency parts of spectra increase with height in the surface layer, while in the self similar (k₁ ^-1) range the streamwise low frequency components are approximately constant with height. These large-scale longitudinal eddies extend to a length _s, which is equal to the boundary-layer height near the surface andincreases linearly to a maximum of about three times the boundary-layer height at roughly 15 m and decreases in the upper parts of the surface layer. This lower part of the surface layer, the eddy surface layer, is the region in which the eddies impinging from layers above are strongly distorted. This new result for the atmospheric boundary layer has practical application for calculating fluctuating wind loads on structures and lateral dispersion of pollution from local sources.     

Hydrothermal gases offshore Milos Island, Greece

Hydrothermal fluids emerge from the seafloor of Paleohori Bay on Milos. The gases in these fluids contain mostly CO₂ but CH₄ concentrations up to 2% are present. The stable carbon isotopic composition of the CO₂ (near 0%) indicates an inorganic carbon source (dissociation of underlying marine carbonates). The carbon and hydrogen isotopes of most CH₄ samples are enriched in the heavy species (δ¹³C = −9.4 to −17.8‰; δD = −102 to −189‰) which is believed to be characteristic for an abiogenic production of CH₄ by CO₂-reduction (Fischer-Tropsch reactions). Depletions in the deuterium content of three CH₄ samples (to −377%) are probably caused by unknown subsurface rock alteration processes. Secondary hydrogen isotope exchange processes between methane, hydrogen and water are most likely responsible for calculated unrealistic methane formation temperatures.

We show that excess helium, slightly enriched in ³He, is present in the hydrothermal fluids emerging the seafloor of Paleohori Bay. When the isotopic ratio of the excess component is calculated a ³He/⁴He_excess of 3.6 · 10⁻⁶ is obtained: This indicates that the excess component consists of about one third of mantle helium and two thirds of radiogenic helium. We infer that the mantle-derived component has been strongly diluted by radiogenic helium during the ascent of the fluids to the surface.     

Chronometric calibration of a comparative time scale for the mesozoic and paleozoic

The relationship between radiometric and biostratigraphic ages is analyzed by local empirical methods and illustrated in chronograms for the mesozoic and paleozoic stages. The chronograms allow estimates for stage boundaries and their errors. Consequently, the ages for epoch boundaries and their errors are discussed which, for several reasons, may not be identical with the age and error of the oldest stage of an epoch.The analysis results in a comparative time scale which could be a compromise between the time scales currently under discussion. A specific feature of the derived time scale is its rather strong cyclicity with periods around 74 (73±1) ma.

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Zusammenfassung Alter und Fehlergrenzen für mesozoische und paläozoische Stufengrenzen werden mittels einer lokalen empirischen Schätzmethode neu berechnet. Daraus ergeben sich Schätzungen für die Alter und Fehler der Epochengrenzen, die aus verschiedenen Gründen nicht mit denen derältesten Stufe einer Epoche zusammenfallen.Die Auswertung der Chronogramme führt zu einer vergleichenden Zeitskala, die im wesentlichen einen Kompromi\ zwischen den gegenwärtig gängigen Zeitskalen darstellt. Bemerkenswert ist, da\ die Zeitskala ein hohes Ma\ an Zyklizität aufweist, wobei sich Perioden von 74 (73±1) ma ergeben.

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Résumé Les relations entre les âges radiométriques et les âges biostratigraphiques sont analysées par des méthodes locales et empiriques. Elles sont présentées dans des chronogrammes se référant aux étages du MésozoÏque et du PaléozoÏque. Ces chronogrammes permettent d'estimer les limites d'étages et les erreurs de cette estimation. Les âges des limites de systèmes et leurs erreurs sont discutés en conséquence. Pour diverses raisons ils ne sont pas identiques à ceux de l'étage le plus ancien d'un système.L'analyse des chronogrammes aboutit à une échelle de temps comparative qui représente un compromis entre les échelles de temps couramment discutées. Une propriété remarquable de cette échelle est de faire apparaÎtre une cyclicité dont la période est de 74 (73±1) Ma.

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- . , . , . , 74 (73 ± 1) .